Cell Types & Structures: Skills (DP IB Biology) : Revision Note
Cell Types & Structures: Skills
It is important to be able to recognise various organelles from light and electron microscope images
When interpreting micrographs to identify and deduce the function of the cell it is important to:
Identify whether it is a prokaryotic or eukaryotic cell - look to see if a nucleus is present or not
Identify which type of eukaryotic cell it is (plant or animal) by looking for a cell wall or vacuole
Identify the organelles present in the cells and consider their function
You should be confident in identifying the following structures and organelles:
Nucleoid region in a prokaryotic cell
Prokaryotic cell wall
Nucleus
Mitochondria
Chloroplast
Sap vacuole
Golgi apparatus
Rough and smooth endoplasmic reticulum
Chromosomes
Ribosomes
Plant cell wall
Plasma membrane
Microvilli
Some identifiable features of key organelles are:
Chloroplast
Has distinctive stacks of thylakoids
Double membrane
Has a roughly oval shape
Larger than mitochondria
Indicates the cell is a plant cell
Nucleus
Has a nuclear membrane and a dark nucleolus within
It has a roughly spherical shape
Vacuole
Occupies a large space within a cell
Often shows up as a very light shade (white) within an electron micrograph
Indicates the cell is a plant cell
Cell wall
Located around the perimeter of the cell
Mitochondria
Roughly oval-shaped
Double membrane
Sometimes observed with visible cristae (foldings of the inner membrane)
An interpretation of an electron micrograph of a cell

Electron micrograph of cell 1
To identify this cell consider the following
The cell has a nucleus - therefore it is a eukaryotic cell
This cell does not have a cell wall or central vacuole - therefore it is an animal cell
The cell has a large u-shape nucleus - so it can manipulate itself through small pores
There are a large number of lysosomes in the cell - so it can digest substances found within the cell
There are a large number of mitochondria - this means it has sufficient energy for its many metabolic reactions
The deduction, therefore, is that this cell needs a lot of energy to break down substances that enter the cell and that it can move where it wants. This cell is a macrophage
An interpretation of an electron micrograph of a cell

Electron micrograph of cell 2
To identify this cell consider the following
The cell has a nucleus - therefore it is a eukaryotic cell
This cell does not have a cell wall or central vacuole - therefore it is an animal cell
There are a large number of mitochondria - so it requires significant energy for many metabolic reactions
The cell has microvilli packed closely together (brush border) - so it needs to increase the surface area and prevent any substance from crossing into the cell
The deduction, therefore, is that this cell needs a lot of energy to control what enters or exits this cell and that the cell requires many of the substance to be absorbed. This cell is a ciliated epithelium of the small intestine
Worked Example
The image below shows a cell as viewed through an electron microscope.
Identify three organelles and the type of cell.

Answer:

Identifiable organelles are:
Nucleus and nucleolus
Mitochondria
Chloroplast
Vacuole
Cell wall
This is a plant cell
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