Osmosis (AQA A Level Biology): Revision Note

Exam code: 7402

Lára Marie McIvor

Written by: Lára Marie McIvor

Reviewed by: Ruth Brindle

Updated on

The process of osmosis

  • Water can move in and out of cells by osmosis

  • Osmosis is defined as:

Osmosis is the net movement of water molecules from a region of higher water potential (dilute solution) to a region of lower water potential (concentrated solution), through a partially permeable membrane

Diagram of a partially permeable membrane showing small water molecules passing through, while large solute molecules cannot pass.
Osmosis and the partially permeable membrane
  • A dilute solution has a high water potential (the left-hand side of the diagram below) and a concentrated solution has a low water potential (the right-hand side of the diagram below)

Diagram illustrating osmosis through a partially permeable membrane, showing water molecules moving into a cell containing sucrose molecules.
Osmosis in cells

Examiner Tips and Tricks

The water potential of pure water (without any solutes) at atmospheric pressure is 0kPa, therefore any solution that has solutes will have a water potential lower than 0kPa (it will be a negative value)

When interpreting questions on water potential, remember – the more negative the water potential, the lower the water potential (the further it is away from pure water ).

Osmosis in plant cells

  • Plant and animal cells are affected differently by osmosis, as plant cells have a cell wall

  • In a hypertonic solution (lower water potential outside), water leaves the plant cell, the protoplast shrinks and pulls away from the cell wall — this is called plasmolysis

    • Without enough water, cells lose turgor and the plant wilts

  • In a hypotonic solution (higher water potential outside), water enters the plant cell, the vacuole expands, and the cell becomes turgid — the cell wall prevents bursting

    • Turgidity supports the plant, helping it stay upright and catch sunlight

  • In an isotonic solution, water moves in and out equally, so there is no net change, and the cell is neither turgid or plasmolysed

Diagram showing plant cell plasmolysis stages: turgid cell, water leaving, protoplast shrinking, fully plasmolysed cell. Annotations detail process.
Osmosis in plant cells

Osmosis in animal cells

  • Animal cells, like plant cells, gain or lose water by osmosis, but the effects are more severe as they lack a cell wall

    • In a hypertonic solution (lower water potential outside), water leaves the cell, causing it to shrink and shrivel

    • In a hypotonic solution (higher water potential outside), water enters the cell, which may swell and burst (cytolysis)

    • In an isotonic solution, water moves in and out equally, so there is no net change to the cell

  • Maintaining a stable water potential in animal tissue fluid is essential to prevent cell damage

    Diagram comparing red blood cell behaviour in hypertonic, isotonic, and hypotonic solutions, showing osmosis effects like shrinking or swelling.
    Osmosis in animal cells

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Lára Marie McIvor

Author: Lára Marie McIvor

Expertise: Biology, Psychology & Sociology Subject Lead

Lára graduated from Oxford University in Biological Sciences and has now been a science tutor working in the UK for several years. Lára has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning.

Ruth Brindle

Reviewer: Ruth Brindle

Expertise: Biology Content Creator

Ruth graduated from Sheffield University with a degree in Biology and went on to teach Science in London whilst also completing an MA in innovation in Education. With 10 years of teaching experience across the 3 key science disciplines, Ruth decided to set up a tutoring business to support students in her local area. Ruth has worked with several exam boards and loves to use her experience to produce educational materials which make the mark schemes accessible to all students.