Osmosis (AQA A Level Biology): Revision Note
Exam code: 7402
The process of osmosis
Water can move in and out of cells by osmosis
Osmosis is defined as:
Osmosis is the net movement of water molecules from a region of higher water potential (dilute solution) to a region of lower water potential (concentrated solution), through a partially permeable membrane

A dilute solution has a high water potential (the left-hand side of the diagram below) and a concentrated solution has a low water potential (the right-hand side of the diagram below)

Examiner Tips and Tricks
The water potential of pure water (without any solutes) at atmospheric pressure is 0kPa, therefore any solution that has solutes will have a water potential lower than 0kPa (it will be a negative value)
When interpreting questions on water potential, remember – the more negative the water potential, the lower the water potential (the further it is away from pure water ).
Osmosis in plant cells
Plant and animal cells are affected differently by osmosis, as plant cells have a cell wall
In a hypertonic solution (lower water potential outside), water leaves the plant cell, the protoplast shrinks and pulls away from the cell wall — this is called plasmolysis
Without enough water, cells lose turgor and the plant wilts
In a hypotonic solution (higher water potential outside), water enters the plant cell, the vacuole expands, and the cell becomes turgid — the cell wall prevents bursting
Turgidity supports the plant, helping it stay upright and catch sunlight
In an isotonic solution, water moves in and out equally, so there is no net change, and the cell is neither turgid or plasmolysed

Osmosis in animal cells
Animal cells, like plant cells, gain or lose water by osmosis, but the effects are more severe as they lack a cell wall
In a hypertonic solution (lower water potential outside), water leaves the cell, causing it to shrink and shrivel
In a hypotonic solution (higher water potential outside), water enters the cell, which may swell and burst (cytolysis)
In an isotonic solution, water moves in and out equally, so there is no net change to the cell
Maintaining a stable water potential in animal tissue fluid is essential to prevent cell damage
Osmosis in animal cells
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