Processing Data in Biology (DP IB Biology): Revision Note

Ruth Brindle

Last updated

Processing data in Biology

  • This is the "calculation" phase of your investigation

  • Data processing involves applying mathematical calculations to your raw data to determine the final values you need to answer your research question

    • For example, calculating a percentage change in mass, a rate of reaction, or a standard deviation

  • The goal is to carry out relevant and accurate calculations, including measures of data spread, and to present the results clearly

Principles of data processing

Carry out relevant and accurate data processing

  • Your processed data should be presented in a new, clearly labelled table

    • It is good practice to show the raw data and processed data in separate tables

  • Show your working clearly

    • For every type of calculation you perform, you must show one full, worked example

  • Calculating a mean

    • When you have repeat trials (replicates), you must calculate a mean (average) value to use in your analysis

    • You should exclude any justified anomalous results from your calculation

  • Calculating percentage change

    • In biology, we often measure the change in a variable relative to its initial value. This is useful for comparing samples that may not have had identical starting values

      • Percentage Change = ((Final Value – Initial Value) / Initial Value) × 100

  • Calculating a rate

    • For enzyme experiments, you usually measure the time taken for an event

    • You must convert this into a rate to analyse the enzyme's activity

      • Rate = 1 / time (units will be s⁻¹) or Rate = change in a variable / time (e.g., cm³ s⁻¹)

  • Calculating measures of dispersion

    • Due to the high variability in biological data, it is important to quantify the spread of your data around the mean

    • Standard deviation (SD) is the most common measure

      • A small SD indicates that the data points are clustered closely around the mean (high precision), while a large SD indicates a wider spread

  • Significant figures

    • Your final calculated answer should be given to a number of significant figures that reflects the precision of the raw data used

      • The general rule is that your final answer should have the same number of significant figures as the least precise piece of data used in the calculation

Worked Example

Research question:

  • "What is the effect of sucrose concentration on the percentage change in mass of potato cylinders?"

Raw data table:

Replicate

Initial Mass / g (±0.01)

Final Mass / g (±0.01)

1

1.91

2.02

2

1.89

1.99

3

1.87

1.98

Processing the data (sample calculation for Replicate 1):

  1. Calculate the change in mass:

    • Change in mass = Final mass – initial mass

    • Change in mass = 2.02 g – 1.91 g = +0.11 g

  2. Calculate the percentage change in mass:

    • % Change = (Change in mass / initial mass) × 100

    • % Change = (0.11 g / 1.91 g) × 100 = +5.76%

Processed data table:

  • This table would show the calculated values for all replicates and conditions

Sucrose Conc. / M

Mean % Change in Mass

Standard Deviation of % Change

0.0

+14.8

0.4

0.2

+5.5

0.3

0.4

-3.2

0.5

0.6

-10.1

0.4

0.8

-15.6

0.6

Worked Example

Research question:

  • "What is the effect of pH (from pH 4 to pH 10) on the rate of activity of the enzyme trypsin in breaking down casein protein?"

Raw data table:

pH (±0.1)

Time (Trial 1) /s (±0.2)

Time (Trial 2) /s (±0.2)

Time (Trial 3) /s (±0.2)

Time (Trial 4) /s (±0.2)

4.0

185.4

182.9

184.1

5.0

158.3

160.2

159.0

6.0

102.5

101.8

103.1

7.0

63.4

62.8

63.0

8.0

46.9

52.6 (anomalous)

47.0

47.1

9.0

65.1

64.8

65.9

10.0

150.5

148.8

149.3

Processing the data (sample calculation for pH 7.0):

  1. Calculate the average time :

    • Average time = fraction numerator 63.4 plus 62.8 plus 63.0 over denominator 3 end fraction = 63.1 s

  2. Calculate the rate of reaction

    • Rate = 1 / time

    • Rate = fraction numerator 1 over denominator 63.1 end fraction = 0.01584786 s-1

    • Given the raw data has 3 s.f., the rate should be 0.0158 s-1

Final processed data table:

pH (±0.1)

Mean Time /s

Rate (1/time) /s⁻¹

4.0

184.1

0.0054

5.0

159.2

0.0063

6.0

102.5

0.0098

7.0

63.1

0.0158

8.0

47.0 (excluding anomaly)

0.0213

9.0

65.3

0.0153

10.0

149.5

0.0067

Examiner Tips and Tricks

  • Show one full worked example

    • Even if you use a spreadsheet, you must show the assessor how you got from your raw data to your processed data for one set of values.

    • This is essential for gaining full marks

  • Watch your significant figures

    • A common error is to write down the full calculator display

    • Always round your final answer to the correct number of significant figures based on your raw data

  • Include a measure of spread

    • For a high-scoring biology IA, processing should go beyond just calculating the mean

    • Calculating the standard deviation for your replicates is essential to show the variability in your data

  • Separate raw and processed data

    • Presenting your initial measurements in one table and your calculated results in a second, final table makes your report much clearer and easier to follow

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Ruth Brindle

Author: Ruth Brindle

Expertise: Biology Content Creator

Ruth graduated from Sheffield University with a degree in Biology and went on to teach Science in London whilst also completing an MA in innovation in Education. With 10 years of teaching experience across the 3 key science disciplines, Ruth decided to set up a tutoring business to support students in her local area. Ruth has worked with several exam boards and loves to use her experience to produce educational materials which make the mark schemes accessible to all students.