Collecting Data in Biology (DP IB Biology): Revision Note
Concluding in Biology
The conclusion is a brief, focused summary of the findings of your investigation
Your goal is to provide a clear and concise answer to your research question, based only on the evidence from your data analysis
A strong conclusion relates your specific findings back to broader biological principles and compares them to established patterns or published data
Principles of concluding
Interpret processed data and analysis to draw and justify conclusions
Your conclusion must be justified by your data
This is the most important rule
Start your conclusion with a direct answer to your research question
Use the trend you identified in your interpretation to make a definitive statement
Include key processed data as evidence to support your statement, such as:
the final calculated value (e.g. the isotonic point of a tissue)
the optimal value from your graph (e.g. the optimal temperature for an enzyme)
key values that demonstrate the trend (e.g. the mean percentage change in mass at the lowest and highest concentrations)
Never introduce new ideas or explanations in the conclusion
It should only be a summary of what you have already analysed
Relate the outcomes to the stated research question or hypothesis
You must explicitly state whether your results support or refute your initial hypothesis
For example: "The data shows that the enzyme's activity peaked at pH 8 and decreased at higher and lower pH values, which supports the hypothesis."
If your results do not support your hypothesis, that is perfectly fine and does not mean your experiment has "failed"
It is a valid scientific finding, and you should state it clearly
You can then suggest reasons for the discrepancy in your evaluation
Compare the outcomes to the accepted scientific context
A high-level conclusion compares your experimental results to accepted literature values from the IB data booklet, textbooks, or other reliable sources
This comparison allows you to comment on the accuracy of your outcome
When making a comparison, you should:
State the expected pattern or published value
Cite your source (e.g. "Campbell Biology, 12th Edition")
If comparing to a specific value, you can quantify the difference by calculating the percentage error
Discuss the impact of uncertainties on the conclusions
The spread of your data, represented by standard deviation and error bars, determines the confidence you have in your conclusion
If error bars are small and do not overlap between different conditions, you can be more confident that the difference you have observed is significant
For example:
The small standard deviations for all measurements, as shown by the error bars on the graph, indicate that the data is precise and the identified trends are reliable
The non-overlapping error bars between the pH 7 and pH 8 conditions suggest that the increase in enzyme activity is statistically significant
If error bars are large or overlap between different conditions, you can be less confident that the differences you observe are significant
The variation in the data means the apparent trend might be due to random error rather than a true effect
For example:
The large standard deviations shown by the error bars on the graph suggest that the data are less precise, and there may have been inconsistency between trials
The overlapping error bars between the pH 7 and pH 8 conditions indicate that the difference in enzyme activity may not be statistically significant, and additional repeats would help confirm whether the observed pattern is reliable
Note that these examples are for illustrative purposes and are not representative of the data presented above
Worked Example
Research question:
"What is the isotonic point of potato tuber tissue in a sucrose solution?"
Sample conclusion:
The results show a clear negative correlation between the sucrose concentration and the percentage change in mass of the potato cylinders.
This supports the hypothesis that as the external solute concentration increases, the potato tissue will lose more water via osmosis.
From the x-intercept of the graph of mean percentage change in mass versus sucrose concentration, the isotonic point for the potato tuber tissue was determined to be 0.35 M.
This value represents the concentration at which the water potential of the potato tissue is equal to that of the surrounding solution.
The small standard deviations, as illustrated by the small error bars on the graph, indicate that the data is precise and the determined isotonic point is reliable.
This finding is consistent with established biological principles of osmosis in plant tissues.
Worked Example
Research question:
"What is the effect of pH (from pH 4 to pH 10) on the rate of activity of the enzyme trypsin in breaking down casein protein?"
Sample conclusion:
The investigation found that the rate of trypsin activity decreased at higher and lower values on either side of the peak rate of activity (pH 8)
The graph of reaction rate versus pH shows that the optimum pH for trypsin activity is approximately pH 8.0
This supports the hypothesis that trypsin would show maximum activity near its optimal pH and reduced activity in conditions that are too acidic or too alkaline
This result aligns with the scientific understanding that enzymes have an active site structure adapted to a specific pH range
Changes in hydrogen ion concentration at extreme pH values can disrupt ionic and hydrogen bonds, altering the enzyme’s tertiary structure and the shape of its active site
The decrease in activity above and below pH 8.0 is attributed to denaturation of the enzyme under increasingly alkaline or acidic conditions
The small variation in the mean rates across replicates suggests that the data were precise and reliable, giving confidence in the conclusion
Examiner Tips and Tricks
Be concise and direct
A conclusion should be a short paragraph, not a long essay
Get straight to the point and answer your research question
Only conclude what your data shows
Do not make claims that are not supported by your results
If your data is inconclusive (e.g. due to very large error bars), you must state that
Always include your key final result
Stating the final calculated value (e.g., the optimum pH or the isotonic point) is crucial evidence
Refer to the reliability of your data
Briefly mentioning that your conclusion is reliable due to small standard deviations or concordant results will strengthen your report
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