Collecting Data in Biology (DP IB Biology): Revision Note

Ruth Brindle

Last updated

Concluding in Biology

  • The conclusion is a brief, focused summary of the findings of your investigation

  • Your goal is to provide a clear and concise answer to your research question, based only on the evidence from your data analysis

  • A strong conclusion relates your specific findings back to broader biological principles and compares them to established patterns or published data

Principles of concluding

Interpret processed data and analysis to draw and justify conclusions

  • Your conclusion must be justified by your data

    • This is the most important rule

  • Start your conclusion with a direct answer to your research question

  • Use the trend you identified in your interpretation to make a definitive statement

  • Include key processed data as evidence to support your statement, such as:

    • the final calculated value (e.g. the isotonic point of a tissue)

    • the optimal value from your graph (e.g. the optimal temperature for an enzyme)

    • key values that demonstrate the trend (e.g. the mean percentage change in mass at the lowest and highest concentrations)

  • Never introduce new ideas or explanations in the conclusion

    • It should only be a summary of what you have already analysed

Relate the outcomes to the stated research question or hypothesis

  • You must explicitly state whether your results support or refute your initial hypothesis

    • For example: "The data shows that the enzyme's activity peaked at pH 8 and decreased at higher and lower pH values, which supports the hypothesis."

  • If your results do not support your hypothesis, that is perfectly fine and does not mean your experiment has "failed"

    • It is a valid scientific finding, and you should state it clearly

    • You can then suggest reasons for the discrepancy in your evaluation

Compare the outcomes to the accepted scientific context

  • A high-level conclusion compares your experimental results to accepted literature values from the IB data booklet, textbooks, or other reliable sources

  • This comparison allows you to comment on the accuracy of your outcome

  • When making a comparison, you should:

    • State the expected pattern or published value

    • Cite your source (e.g. "Campbell Biology, 12th Edition")

    • If comparing to a specific value, you can quantify the difference by calculating the percentage error

Discuss the impact of uncertainties on the conclusions

  • The spread of your data, represented by standard deviation and error bars, determines the confidence you have in your conclusion

    • If error bars are small and do not overlap between different conditions, you can be more confident that the difference you have observed is significant

    • For example:

      • The small standard deviations for all measurements, as shown by the error bars on the graph, indicate that the data is precise and the identified trends are reliable

      • The non-overlapping error bars between the pH 7 and pH 8 conditions suggest that the increase in enzyme activity is statistically significant

    • If error bars are large or overlap between different conditions, you can be less confident that the differences you observe are significant

    • The variation in the data means the apparent trend might be due to random error rather than a true effect

    • For example:

      • The large standard deviations shown by the error bars on the graph suggest that the data are less precise, and there may have been inconsistency between trials

      • The overlapping error bars between the pH 7 and pH 8 conditions indicate that the difference in enzyme activity may not be statistically significant, and additional repeats would help confirm whether the observed pattern is reliable

    • Note that these examples are for illustrative purposes and are not representative of the data presented above

Worked Example

Research question:

  • "What is the isotonic point of potato tuber tissue in a sucrose solution?"

Sample conclusion:

  • The results show a clear negative correlation between the sucrose concentration and the percentage change in mass of the potato cylinders.

    • This supports the hypothesis that as the external solute concentration increases, the potato tissue will lose more water via osmosis.

  • From the x-intercept of the graph of mean percentage change in mass versus sucrose concentration, the isotonic point for the potato tuber tissue was determined to be 0.35 M.

    • This value represents the concentration at which the water potential of the potato tissue is equal to that of the surrounding solution.

  • The small standard deviations, as illustrated by the small error bars on the graph, indicate that the data is precise and the determined isotonic point is reliable.

    • This finding is consistent with established biological principles of osmosis in plant tissues.

Worked Example

Research question:

  • "What is the effect of pH (from pH 4 to pH 10) on the rate of activity of the enzyme trypsin in breaking down casein protein?"

Sample conclusion:

  • The investigation found that the rate of trypsin activity decreased at higher and lower values on either side of the peak rate of activity (pH 8)

    • The graph of reaction rate versus pH shows that the optimum pH for trypsin activity is approximately pH 8.0

  • This supports the hypothesis that trypsin would show maximum activity near its optimal pH and reduced activity in conditions that are too acidic or too alkaline

  • This result aligns with the scientific understanding that enzymes have an active site structure adapted to a specific pH range

    • Changes in hydrogen ion concentration at extreme pH values can disrupt ionic and hydrogen bonds, altering the enzyme’s tertiary structure and the shape of its active site

    • The decrease in activity above and below pH 8.0 is attributed to denaturation of the enzyme under increasingly alkaline or acidic conditions

  • The small variation in the mean rates across replicates suggests that the data were precise and reliable, giving confidence in the conclusion

Examiner Tips and Tricks

Be concise and direct

  • A conclusion should be a short paragraph, not a long essay

  • Get straight to the point and answer your research question

Only conclude what your data shows

  • Do not make claims that are not supported by your results

  • If your data is inconclusive (e.g. due to very large error bars), you must state that

Always include your key final result

  • Stating the final calculated value (e.g., the optimum pH or the isotonic point) is crucial evidence

Refer to the reliability of your data

  • Briefly mentioning that your conclusion is reliable due to small standard deviations or concordant results will strengthen your report

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Ruth Brindle

Author: Ruth Brindle

Expertise: Biology Content Creator

Ruth graduated from Sheffield University with a degree in Biology and went on to teach Science in London whilst also completing an MA in innovation in Education. With 10 years of teaching experience across the 3 key science disciplines, Ruth decided to set up a tutoring business to support students in her local area. Ruth has worked with several exam boards and loves to use her experience to produce educational materials which make the mark schemes accessible to all students.