Limiting Factors Affecting Enzymes: Enzyme Concentration (AQA AS Biology): Revision Note

The effect of enzyme concentration

• Enzyme concentration affects the rate of reaction

• The higher the enzyme concentration in a reaction mixture, the greater the number of active sites available and the greater the likelihood of enzyme-substrate complex formation

• As long as there is sufficient substrate available, the initial rate of reaction increases linearly with enzyme concentration

• If the amount of substrate is limited, further increases in enzyme concentration will not increase the reaction rate as the amount of substrate becomes a limiting factor