Exam code: 7402
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Define the term ecosystem.
A community and its interactions with the non-living (abiotic) factors in the environment.

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Define the term population.
A group of organisms of the same species living in a particular space at a particular time that can potentially interbreed.
Define the term community.
Multiple populations living and interacting in the same area.
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Define the term ecosystem.
A community and its interactions with the non-living (abiotic) factors in the environment.
Define the term population.
A group of organisms of the same species living in a particular space at a particular time that can potentially interbreed.
Define the term community.
Multiple populations living and interacting in the same area.
Define the term habitat.
The local environment in which a species normally lives.
Define the term niche.
The role an organism plays in its ecosystem, including its use of resources, its responses to abiotic factors and its interactions with biotic factors.
How many species can occupy a particular niche?
Only one species can occupy a particular niche.
Each species has a unique niche.
Define biotic factors.
The living components of an ecosystem that affect the survival and reproduction of organisms.
Examples include predation, competition, disease and food availability.
Define abiotic factors.
The non-living components of an ecosystem that affect living organisms.
Examples include temperature, light intensity, pH, water availability and mineral ions.
What happens if the niches of two species overlap?
The two species compete with each other.
This can result in either:
One species outcompeting the other, so the less successful species adapts to a new niche or becomes locally extinct.
Both populations continuing to exist, but with smaller population sizes than they would have without competition.
What is meant by interdependence within a community?
Within a community, each species depends on other species (e.g. for food, shelter and pollination).
If one species is removed it can affect the whole community.
Three North American warbler species coexist in the same conifer habitat by feeding at different in the trees, an example of niche .
Three North American warbler species coexist in the same conifer habitat by feeding at different heights in the trees, an example of niche differentiation.
True or False?
A species' niche is determined by its adaptations, which may be structural, physiological or behavioural.
True.
Adaptations are structural, physiological or behavioural traits that allow survival under specific conditions, and these determine a species' niche.
Define carrying capacity.
The maximum stable population size of a species that an ecosystem can support.
On a population growth curve, which feature represents the carrying capacity?
The point at which the growth curve flattens out (plateaus).
At this point, abiotic and biotic factors prevent the population from growing any further.
Why do most species remain at or below their carrying capacity?
Because abiotic and biotic factors (limiting factors) limit the survival and reproduction of individuals.
This prevents all individuals from surviving and reproducing, so the population cannot keep growing.
Give three examples of abiotic factors that can limit population size.
Any three of:
Light availability
Water supply
Temperature
Amount of space available
Soil pH
Explain how a temperature far from the optimum can limit the population size of a mammal.
Individuals use more energy to maintain their optimum body temperature via homeostasis.
This leaves less energy for growth and reproduction.
Fewer individuals reach reproductive age, so population growth is limited.
Define interspecific competition.
Competition between different species for the same resources (e.g. food, space or nesting sites).
Define intraspecific competition.
Competition between individuals of the same species for the same resources.
How does intraspecific competition cause a population to stabilise at its carrying capacity?
When resources are plentiful, the population increases.
More individuals then compete for food and shelter, so resources become limiting.
This limits further growth, so the population stabilises at the carrying capacity.
Why do grey squirrels outcompete red squirrels in the UK?
Grey squirrels eat a wider range of food.
They are also larger and have better fat storage in winter.
This gives them greater survival and reproduction, so the red squirrels are outcompeted.
Describe the stages of a predator-prey cycle in a stable community.
Prey numbers rise, providing more food for predators.
Predator numbers rise, so more prey is eaten.
Prey numbers fall, so there is less food for predators.
Predator numbers fall, reducing the pressure on the prey.
Prey numbers rise again and the cycle repeats.
True or False?
Predation is an example of an abiotic factor.
False.
Predation is a biotic factor, as it involves an interaction between living organisms.
Competition between two different species for the same resource is called competition, whereas competition between members of the same species is called competition.
Competition between two different species for the same resource is called interspecific competition, whereas competition between members of the same species is called intraspecific competition.
Why is sampling used to estimate population size in large ecosystems?
In large, complex ecosystems it is impossible to find, identify and count every organism.
Instead, samples of the area are taken and used to estimate the total numbers present.
What is the advantage of random sampling over systematic sampling?
In random sampling, the positions of the sampling points are chosen due to chance.
This means there is no bias from the person carrying out the sampling that could affect the results.
State the three main sampling methods used to estimate population size, and when each is used.
Quadrats - for non-motile or slow-moving species.
Transects - for non-motile or slow-moving species.
Mark-release-recapture - for motile species.
When is random sampling using frame quadrats the most appropriate method?
When the sampling area is reasonably uniform or has no clear pattern in the way the species are distributed.
How is the species frequency calculated from quadrat data?
Divide the number of quadrats the species was present in by the total number of quadrats, then multiply by 100.
For example, bluebells found in 18 out of 50 quadrats = (18 / 50) x 100 = 36%.
How is the percentage cover of a species estimated using a quadrat?
The quadrat is divided into 100 smaller squares.
The number of squares the species is found in is equivalent to its percentage cover in that quadrat.
When is a belt transect a more appropriate sampling method than random quadrats?
When there are changes in the physical (abiotic) conditions across the area, such as changes in altitude, soil pH or light intensity.
A belt transect shows how the distribution or abundance of a species changes along the gradient.
Describe how a belt transect is carried out.
A line is set up using a measuring tape across the area.
Quadrats are placed at regular intervals along the tape.
The abundance of each species in each quadrat is recorded, producing quantitative data.
State the formula used in the mark-release-recapture method (the Lincoln index).
Population size = (number in first sample x number in second sample) / number marked in the second sample.
236 leafhoppers are marked and released. A second sample of 244 is caught, of which 71 are marked. Estimate the population size.
Substitute the values into the formula:
N = (n1 x n2) / m2
N = (236 x 244) / 71
N = 811 leafhoppers
Describe the steps of the mark-release-recapture technique.
A first large sample is caught, counted and marked in a way that will not affect survival.
The marked individuals are released and allowed to mix randomly with the population.
After sufficient time, a second large sample is caught.
The number of marked and unmarked individuals is counted and used to estimate the population size.
State the assumptions made when using the mark-release-recapture method.
Marked individuals have sufficient time to disperse and mix back into the population.
The marking does not affect survival rates (e.g. does not make individuals more likely to be predated).
The marking remains visible and does not rub off.
The population size stays constant, with no significant births, deaths or migration.
Define succession.
The gradual change in an ecosystem over time, from a simple to a more complex structure.
What is primary succession?
Succession that occurs on newly formed or exposed land with no initial life (e.g. bare rock from cooled lava or a dried-up lakebed).
What are pioneer species?
The first species to colonise newly exposed land (often mosses and lichens).
Their death and decay forms basic soil (humus).
Describe how pioneer species make bare rock more suitable for later species.
Pioneer species (e.g. mosses and lichens) colonise the bare rock and grow.
When they die and decay, they form basic soil (humus).
This allows small plants and grasses to grow, whose roots hold the soil in place and further increase soil depth and nutrient content.
What is a climax community?
The final, stable, complex ecosystem formed at the end of succession, containing a variety of plant and animal species and dominated by the final species (e.g. large trees).
How does each stage of succession affect the environment for the next community?
Each stage changes the abiotic and biotic conditions, for example by building deeper, more nutrient-rich soil.
This makes the environment less hostile and more suitable for the next community.
At the same time, it may become less suitable for the previous species.
True or False?
During primary succession, biodiversity generally increases as succession progresses towards the climax community.
True.
As succession progresses, the environment becomes less hostile and can support a greater variety of species, so biodiversity increases.
How do mowing and grazing affect succession?
They interrupt succession, maintaining the ecosystem in an earlier stage.
Mowing prevents shrubs and trees from establishing, so only grasses persist.
Grazing livestock eat new shoots, halting succession and maintaining grass-dominated areas.
Why does conservation often involve deliberately halting succession?
Because intermediate stages (e.g. grassland, heathland) can support many species not found in climax communities.
In a climax community, dominant species may outcompete others, or changed abiotic conditions may no longer suit some species.
Intermediate habitats can also be important for rare or threatened species, including pollinators like bees.
Using the Scottish moorlands as an example, explain why succession may be deliberately managed.
Naturally, the moorland would progress to spruce forest via succession.
However, the moorland supports unique biodiversity not found in spruce forests.
Management maintains both climax forest and earlier moorland to maximise species diversity.
State two methods used to deliberately prevent succession for conservation.
Grazing - introducing animals to eat tree and shrub shoots, halting succession.
Managed burning - using controlled fires to remove woody plants, allowing species like heather to regrow and resetting succession.
The first species to colonise bare rock are called species, and the final stable stage of succession is known as the community.
The first species to colonise bare rock are called pioneer species, and the final stable stage of succession is known as the climax community.
What is meant by turbidity?
A measure of the cloudiness of a suspension (i.e. how much light can pass through it).
How can turbidity be used to estimate the population size of microorganisms in a broth culture?
As the microorganisms reproduce and their population grows, the suspension becomes more turbid (cloudy).
Measuring how much light passes through the suspension at fixed time intervals therefore estimates the number of cells.
Name three pieces of apparatus that can be used to measure the turbidity of a broth culture.
Any of:
A turbidity meter
A light sensor
A colorimeter (connected to a datalogger)
Describe how the growth rate of microorganisms is investigated using turbidity.
The microorganisms are grown in a broth culture.
After the initial inoculation of the nutrient broth, the turbidity is measured at fixed time intervals.
The results are used to plot a population growth curve showing how the population changed over time.
Why are logarithmic scales useful when investigating bacterial growth?
Bacteria can grow at rapid rates, producing very large numbers within hours.
This gives a wide range of very small and very large numbers, making a suitable linear scale hard to choose.
A logarithmic scale allows these large changes to be shown on a compressed axis.
By what factor does each interval increase on a logarithmic scale?
Each interval increases by powers of 10.
For example: 102 = 100 and 103 = 1000.
How can you recognise a logarithmic scale on a graph?
The intervals on the axis are not evenly spaced in terms of the actual values.
The spacing between powers of 10 is even, but the numbers in between are not linear.
True or False?
The pH scale is an example of a logarithmic scale.
True.
The pH scale is logarithmic; the concentration of hydrogen ions varies massively between each pH level.
True or False?
In the exam you may be asked to convert values into logarithms and draw a log-scale graph yourself.
False.
You will not be expected to convert values into logarithms or create a log-scale graph.
You may instead be asked to interpret results that use logarithmic scales or explain the benefit of using one.
As microorganisms reproduce in a broth culture, the suspension becomes more , meaning that light can pass through it.
As microorganisms reproduce in a broth culture, the suspension becomes more turbid, meaning that less light can pass through it.
A logarithmic scale increases by powers of , and can be recognised because its intervals are not evenly .
A logarithmic scale increases by powers of ten, and can be recognised because its intervals are not evenly spaced.
Why is there a conflict between human needs and conservation?
Humans use Earth's resources (e.g. land, water, wood and fossil fuels) for needs such as buildings, agriculture, fuel and electricity.
As population and economic development increase, demand rises, causing environmental damage to ecosystems, climate and biodiversity.
What is conservation?
The sustainable management of species and habitats, meeting present needs without compromising the future.
What does it mean to manage resources sustainably?
Managing resources so that present needs are met without compromising the ability of future generations to meet their own needs.
How do national and marine parks help manage the conflict between human needs and conservation?
They protect habitats with legal restrictions on access, development and hunting.
This balances biodiversity with controlled land use.
How do zoos contribute to conservation?
Through captive breeding, which helps restore species populations and supports research.
This reduces the pressure on wild populations.
How do seed banks support conservation?
They store plant diversity safely.
This allows future crop restoration and species recovery, offsetting habitat loss.
How does public engagement support conservation?
It generates income through tourism and provides local jobs and funding for services.
This increases community support for conservation.
Why is data from a single study usually not enough to justify conservation action?
One study does not provide sufficiently certain conclusions.
Results from multiple similar studies are normally required; if these results agree, a more certain conclusion can be drawn.
Why does including a control site increase the validity of a conservation investigation?
A control site (where no intervention occurs) allows other variables (abiotic factors like water quality and biotic factors like predation) to be discounted.
This increases the validity of the results.
Why is random sampling used when estimating population numbers in a conservation study?
So that the data is not biased.
This gives a more accurate estimate for the whole area.
Give two reasons why a short-term conservation study over a small area may give unreliable conclusions.
A decline over a short period could simply be a natural population fluctuation that may reverse over a longer time period.
A smaller study area means population size estimates may be less accurate.
Conservation involves the management of species and habitats, meeting present needs without compromising the .
Conservation involves the sustainable management of species and habitats, meeting present needs without compromising the future.
Name two types of factor whose effect on species distribution can be investigated in this required practical.
Abiotic factors (e.g. light intensity, nutrient concentration, temperature).
Biotic factors (e.g. intraspecific or interspecific competition, predators or pathogens).
When should random sampling with frame quadrats be used, and when should systematic sampling with belt transects be used?
Random sampling (frame quadrats) - when there are no clear or obvious changes in the physical conditions across the area.
Systematic sampling (belt transects) - when there is an obvious change in an abiotic factor (e.g. altitude or light intensity) across the area.
What apparatus would you use to measure soil temperature, soil pH and light intensity?
Soil temperature - a digital thermometer.
Soil pH - a digital pH meter.
Light intensity - a photometer.
Describe how to carry out random sampling using frame quadrats to investigate species distribution.
Set up two tape measures as x and y axes to form a 5m by 5m grid.
Use a random number generator to generate 10 sets of random coordinates.
At each coordinate, place the quadrat and record the percentage cover of the plant species and a measurement of the abiotic factor.
Describe how to carry out systematic sampling using a belt transect.
Place a tape measure as a transect running from one extreme of the abiotic factor to the other (e.g. from under the tree canopy to open ground).
At regular intervals (e.g. every 1m), place a quadrat.
Record the percentage cover of the plant species and a measurement of the abiotic factor at each point.
Why is a random number generator used to place quadrats in random sampling?
To select the coordinates for the quadrats randomly.
This avoids sampling bias, giving a more representative estimate of the area.
How is percentage cover recorded using a quadrat divided into 100 squares?
The number of the 100 small squares in which the species is found is equivalent to its percentage cover in that quadrat.
How are the results of this practical presented and analysed?
Plot a graph of the percentage cover of the species (dependent variable) against the abiotic factor (independent variable).
A correlation indicates the effect of the abiotic factor on distribution.
A statistical test (e.g. Spearman's Rank Correlation) can test whether the correlation is statistically significant.
True or False?
A statistically significant correlation between an abiotic factor and species distribution proves the factor causes the distribution.
False.
Correlation does not always mean causation.
The correlation could be due to chance, or to other abiotic or biotic factors influencing the results.
Give an example of why further investigation may be needed before drawing conclusions from this practical.
A change in species abundance (e.g. moving from woodland to open field) may not be due to the investigated factor (e.g. light intensity) alone.
Other factors, such as a change in soil pH or the presence of a specific pollinator, could also be responsible.
A belt transect is used for sampling when there is an obvious gradient in an abiotic factor, whereas frame quadrats placed using a random number generator are used for sampling.
A belt transect is used for systematic sampling when there is an obvious gradient in an abiotic factor, whereas frame quadrats placed using a random number generator are used for random sampling.
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