Exam code: 9BN0
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Name three structures found in plant cells but not animal cells.
A cellulose cell wall.
A large permanent vacuole.
Chloroplasts.

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What is the function of the plant cell wall?
It provides structural support to the cell.
This is provided by the polysaccharide cellulose.
Define middle lamella.
The middle lamella is the outermost layer of a plant cell that acts like glue, sticking adjacent cells together for stability.
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Name three structures found in plant cells but not animal cells.
A cellulose cell wall.
A large permanent vacuole.
Chloroplasts.
What is the function of the plant cell wall?
It provides structural support to the cell.
This is provided by the polysaccharide cellulose.
Define middle lamella.
The middle lamella is the outermost layer of a plant cell that acts like glue, sticking adjacent cells together for stability.
Define plasmodesmata.
Plasmodesmata are narrow threads of cytoplasm connecting neighbouring plant cells, allowing transport and communication between them.
What is the function of a chloroplast?
It is the site of photosynthesis.
Its thylakoids contain the pigment chlorophyll.
Describe the internal structure of a chloroplast.
Thylakoids stack into structures called grana, joined by lamellae.
These sit in a fluid called the stroma, all within a double membrane.
Define amyloplast.
An amyloplast is a membrane-bound plant organelle that stores starch and converts it back to glucose when needed.
What is the function of the plant vacuole?
It stores cell sap and keeps the cell turgid, stopping the plant wilting.
It can also break down and isolate unwanted chemicals.
Define tonoplast.
The tonoplast is the selectively permeable membrane surrounding the plant vacuole, controlling what enters and leaves.
What are pits in a plant cell wall?
Very thin regions of the cell wall.
They line up in adjacent cells to allow the transport of substances between them.
The green pigment found in chloroplasts, needed for photosynthesis, is .
The green pigment found in chloroplasts, needed for photosynthesis, is chlorophyll.
True or False?
Both chloroplasts and amyloplasts are found in plant cells.
True.
Chloroplasts carry out photosynthesis and amyloplasts store starch — both are plant organelles.
What are the two functions of xylem tissue?
Transport of water and dissolved minerals up the plant.
Structural support.
Why are mature xylem vessels well suited to transporting water?
They are made of dead cells with no cytoplasm or organelles.
This forms hollow tubes so water can flow freely.
What is the role of lignin in xylem vessels?
It strengthens the cell walls.
This lets the vessels withstand the pressure of the moving water column and gives support.
Define translocation.
Translocation is the transport of organic compounds such as sucrose through the phloem, from sources to sinks.
What is the function of phloem tissue?
To transport organic compounds (assimilates), mainly sucrose.
It moves them from sources (e.g. leaves) to sinks (e.g. roots), up or down the plant.
Name the two main cell types of phloem tissue.
Sieve tube elements and companion cells.
What is the role of companion cells?
They control the metabolism of their sieve tube element.
They help load and unload sugars into the phloem.
What is the function of sclerenchyma fibres?
To provide support to the plant.
They are dead cells with lignified walls and extra cellulose, but are not involved in transport.
How are xylem and phloem arranged in a stem?
Xylem is towards the middle of the stem.
Phloem is nearer the outside.
How does xylem differ from phloem in terms of living cells?
Mature xylem is made of dead cells with lignified walls.
Phloem contains living cells with sieve plates and companion cells.
Xylem and phloem are grouped together in a stem to form a bundle.
Xylem and phloem are grouped together in a stem to form a vascular bundle.
True or False?
Phloem transports substances in only one direction.
False.
Phloem transports organic compounds both up and down the plant, from sources to sinks.
Give two roles of water in a plant.
It is a reactant in photosynthesis and a transport medium for minerals.
It also keeps cells turgid and helps regulate temperature.
Why do plants need magnesium ions?
For the production of chlorophyll.
Chlorophyll gives the green colour and is essential for photosynthesis.
Why do plants need nitrate ions?
To synthesise DNA, proteins (including enzymes) and chlorophyll.
These are essential for growth and for making fruit and seeds.
Why do plants need calcium ions?
They are an important cell wall component.
They are needed for proper plant growth.
What symptom would a magnesium-deficient plant show, and why?
Yellow leaves (chlorosis).
Without magnesium, chlorophyll cannot be made.
How are water and minerals absorbed and transported in a plant?
They are absorbed through the root hairs.
They then travel up the stem in the xylem vessels.
In the mineral deficiency practical, why must the seedlings be the same age and from the same plant?
To control variables.
This ensures any difference in growth is due to the mineral concentration, not the plants themselves.
Why are the test tubes of nutrient broth covered with aluminium foil?
To keep light away from the broth.
This prevents the growth of algae, which could use up the minerals.
How is the effect of a mineral deficiency measured in the practical?
By recording each seedling's mass before and after.
The mean change in mass is compared between the different mineral concentrations.
A plant lacking nitrate ions cannot make proteins, chlorophyll or .
A plant lacking nitrate ions cannot make proteins, chlorophyll or DNA.
True or False?
A plant deficient in a mineral ion may show stunted growth.
True.
Mineral deficiencies commonly cause symptoms such as stunted growth and yellowing leaves.
What controls would you include when investigating the effect of a completely missing mineral?
One broth containing all the minerals.
One broth containing no minerals.
Which two polysaccharides make up starch?
Amylose and amylopectin.
Describe the structure of amylose.
An unbranched, helix-shaped chain of alpha glucose.
It contains only 1,4 glycosidic bonds.
Describe the structure of amylopectin.
A branched chain of alpha glucose.
It has 1,4 glycosidic bonds along the chains and 1,6 glycosidic bonds at the branch points.
Why is starch a good storage molecule in plants?
It is compact, so large amounts can be stored.
It is insoluble, so it has no osmotic effect on the cell.
Which type of glucose makes up cellulose, and which bonds join it?
Beta glucose, joined by 1,4 glycosidic bonds.
Why must alternate beta glucose molecules be rotated 180° in cellulose?
Beta glucose is an isomer of alpha glucose.
Rotating alternate molecules allows the 1,4 glycosidic bonds to form.
Explain how cellulose gains its strength.
The inverted beta glucose chains lie parallel.
Many hydrogen bonds form between the chains, grouping them into strong microfibrils.
What is the function of cellulose in a plant?
It is the main structural component of the cell wall.
Its high tensile strength lets cell walls withstand turgor pressure.
How does high tensile strength help cellulose in the cell wall?
It can be stretched without breaking.
This lets the cell wall resist the internal turgor pressure of the cell.
Starch is made of alpha glucose, whereas cellulose is made of glucose.
Starch is made of alpha glucose, whereas cellulose is made of beta glucose.
True or False?
Cellulose is used as an energy store in plants.
False.
Cellulose is a structural molecule in the cell wall; starch is the energy store.
Why does starch take longer to digest than glucose?
It is a large polymer of thousands of glucose monomers.
These bonds must be hydrolysed before the glucose can be used.
Which two plant tissues provide fibres used by humans?
Sclerenchyma fibres and xylem vessels.
Why are plant fibre cells hollow?
Their cell contents have died.
Lignification of the wall makes it almost impossible for substances to enter or leave.
Give two uses of plant fibres by humans.
Making rope.
Making fabrics such as cotton and hemp.
What two features of the cell wall give plant fibres their strength?
The mesh-like arrangement of microfibrils.
Secondary thickening of the wall, often with lignin.
Define secondary thickening.
Secondary thickening is the development of a thick secondary cell wall, often containing lignin, that adds strength to a plant fibre.
How does the arrangement of microfibrils strengthen a plant fibre?
They are arranged in a mesh-like pattern.
This makes the cell wall strong in all directions.
Why does lignin make plant fibres stronger but also kills the cells?
Lignin makes the wall rigid and strong.
But it also makes the wall impermeable, so the cell contents die.
The strength of plant fibres comes partly from the mesh-like arrangement of cellulose .
The strength of plant fibres comes partly from the mesh-like arrangement of cellulose microfibrils.
True or False?
Sclerenchyma fibres are living cells when mature.
False.
Mature sclerenchyma fibres are dead, hollow cells with lignified walls.
Which polysaccharide are the microfibrils in a plant fibre made of?
Cellulose.
Why are the strength properties of plant fibres useful to humans?
Their great strength allows them to be used for products that must bear loads.
Examples are rope and durable fabrics.
True or False?
Xylem vessels can be used as a source of plant fibres.
True.
Both xylem vessels and sclerenchyma fibres are long, strong, hollow cells used as plant fibres.
Why must a very thin cross-section of the stem be cut?
So that light can pass through it under the microscope.
This allows the tissues to be seen clearly.
Why is the stem section stained before viewing?
To make the different tissues visible and distinguishable.
Different tissues take up the stain to different degrees.
With toluidine blue O, what colours do the stem tissues appear?
Xylem and sclerenchyma fibres appear blue-green.
Phloem appears pinkish-purple.
Why should a coverslip be lowered slowly from one side?
To avoid trapping air bubbles.
Air bubbles could be mistaken for plant structures.
What should a labelled drawing of the stem section show?
The positions of the xylem vessels, phloem sieve tubes and sclerenchyma fibres.
Why is the section rinsed in water after staining?
To remove excess stain.
This prevents the image being too dark to see the tissues clearly.
What is an alternative to preparing your own stem slide?
Using a pre-prepared permanent slide of a stem section.
The coverslip should be lowered slowly to avoid trapping bubbles under it.
The coverslip should be lowered slowly to avoid trapping air bubbles under it.
True or False?
Air bubbles under a coverslip could be mistaken for plant tissue.
True.
This is why the coverslip is lowered slowly from one side to avoid trapping them.
Which piece of equipment is used to cut the thin stem section?
A scalpel.
Where in the stem would you expect to find the xylem relative to the phloem?
Xylem is nearer the middle of the stem.
Phloem is nearer the outside.
Why should the stem section be left in the stain for a set time?
To ensure the tissues take up a consistent amount of stain.
This gives a clear, comparable image.
Define tensile strength.
Tensile strength is the maximum load a fibre can carry before it breaks.
Outline how the tensile strength of a plant fibre is measured.
Attach the fibre to a clamp stand and hang a weight from it.
Add weights one at a time until the fibre breaks, and record the mass at which it broke.
What measurement represents the tensile strength in this practical?
The mass (load) at which the fibre breaks.
Why is the practical repeated with several fibre samples?
To increase the accuracy of the results.
A mean tensile strength can then be calculated.
Why must all the fibres be the same length?
To control a variable.
This ensures any difference in strength is due to the fibre type, not its length.
Why is knowing the tensile strength of a plant fibre useful?
It shows how much load a product can bear before breaking.
This matters, for example, when making rope from plant fibres.
Why should weights be added one at a time rather than all at once?
To find the exact load at which the fibre breaks.
Adding gradually gives a more precise measurement.
The tensile strength of a fibre is the maximum it can carry before breaking.
The tensile strength of a fibre is the maximum load it can carry before breaking.
True or False?
Repeating the tensile strength test and taking a mean improves reliability.
True.
Repeating with several samples and calculating a mean reduces the effect of anomalies.
Name one variable, besides fibre length, that should be kept constant.
The type of plant fibre being tested (so only the variable being investigated changes).
What apparatus is used to hold the fibre during the test?
A clamp (retort) stand.
How would you make the comparison of two fibres a fair test?
Keep all variables the same except the fibre type.
Use the same length, add weights the same way and take repeats for each.
Define clinical trial.
A clinical trial is the testing of a new drug for effectiveness and side effects before it can be sold to the public.
What was William Withering's 'digitalis soup'?
Extracts of foxglove (containing digitalis) at different concentrations.
He used them to find the most effective dose to treat dropsy without poisoning the patient.
What condition did Withering treat with digitalis?
Dropsy — swelling caused by heart failure.
What are the first steps of modern drug testing before human trials?
Computer modelling of the drug's effects.
Testing on human tissues in the lab, then on animals.
What happens in Phase 1 of a clinical trial?
The drug is tested on a small group of healthy people.
This checks the body's reaction, side effects and the correct dosage.
What happens in Phase 2 of a clinical trial?
The drug is tested on a larger group of patients who need it.
This checks how effective the drug is.
What happens in Phase 3 of a clinical trial?
The new drug is compared with an existing drug.
A large number of patients are split into two groups, each given one of the drugs.
Define placebo.
A placebo looks exactly like the drug but contains no active ingredient, used to test whether the drug really works.
Define double-blind study.
A double-blind study is one in which neither the patient nor the doctor knows who is receiving the drug or the placebo.
Why are double-blind studies used?
To remove bias from patients and doctors.
Neither knowing the group prevents their expectations affecting the results.
A patient who improves simply because they believe they are receiving a drug is showing the effect.
A patient who improves simply because they believe they are receiving a drug is showing the placebo effect.
True or False?
In a clinical trial, patients are told whether they receive the drug or the placebo.
False.
Patients are not told which group they are in, so the drug's true effect can be measured.
Define antimicrobial.
Antimicrobial describes a substance that kills microbes or prevents their growth.
What conditions do bacteria need to grow and reproduce?
Nutrients and, for aerobes, oxygen.
A suitable temperature and pH for their enzymes to work.
How is a plant extract prepared for the antimicrobial practical?
The plant tissue is dried, ground finely and soaked in ethanol to extract the antimicrobial substances.
The mixture is then filtered.
Define clear zone.
A clear zone is the area around a disc where bacteria cannot grow, showing the antimicrobial effect of the extract.
What does a larger clear zone indicate?
That the plant extract has more effective antimicrobial properties.
What should the control disc be soaked in, and why?
Ethanol only (no plant extract).
This shows that any effect is due to the plant extract, not the ethanol or the disc.
Why is the agar plate incubated at 25 °C rather than body temperature?
25 °C allows good bacterial growth.
It avoids the warmer temperatures that would encourage the growth of human pathogens.
Give two aseptic techniques used in this practical.
Working near a Bunsen flame so rising air carries microbes away.
Using sterile equipment and keeping the plate lid open as little as possible.
Why are aseptic techniques important in this investigation?
To prevent contamination of the culture by other microbes.
Contamination would affect the growth of the bacteria being studied and spoil the results.
How is the size of a clear zone measured?
By measuring its diameter.
The area can then be calculated using πr².
The area around a disc where bacteria cannot grow is called the zone.
The area around a disc where bacteria cannot grow is called the clear zone.
True or False?
All the paper discs should be left in the extract for the same length of time.
True.
This ensures each disc absorbs a similar amount of extract, making the comparison fair.
Define sustainability.
Sustainability is using resources to meet the needs of the current generation without depleting them for future generations.
What is the difference between a renewable and a non-renewable resource?
A renewable resource can be replaced and used without running out (e.g. solar energy).
A non-renewable resource can run out (e.g. fossil fuels).
Why is using plant fibres more sustainable than oil-based plastics?
Fewer fossil fuels are used and plants can be replanted.
Plant fibres are also biodegradable, reducing pollution.
Why is being biodegradable an advantage of plant-fibre products?
They can be broken down by microbes.
This reduces environmental pollution compared with most oil-based plastics.
What is one disadvantage of ropes made from plant fibres?
They are typically not as strong as plastic ropes.
How can starch be used to make a more sustainable alternative to plastics?
Starch can be used to make bioplastics.
These use fewer fossil fuels and come from replantable crops.
What fuel can be made from starch, and why is it more sustainable?
Bioethanol, used as a vehicle fuel.
It comes from renewable crop plants rather than finite oil.
Why are plant-based products often easier to produce in developing countries?
Growing and processing plants is easier and cheaper than extracting and processing oil.
Plastics made from starch instead of oil are called .
Plastics made from starch instead of oil are called bioplastics.
True or False?
Fossil fuels are a renewable resource.
False.
Fossil fuels are non-renewable — they take millions of years to form and can run out.
Define sustainable practice.
A sustainable practice is one that minimises damage to the environment and its resources, such as replanting trees after logging.
Give an example of a sustainable and an unsustainable practice.
Sustainable: replanting trees after logging.
Unsustainable: using fossil fuels to make oil-based plastics.
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