Exam code: YBI11
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Who was Robert Hooke and what is his significance in cell biology?
Robert Hooke was the first person to view cells using a microscope and used the term 'cell' to describe these structures, marking the beginning of cell biology.

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The idea that are made of cells is known as .
The idea that all living organisms are made of cells is known as cell theory.
What are the three main ideas of cell theory?
The three main ideas of cell theory are: all living organisms are made of one or more cells; cells are the basic functional unit of living organisms; and new cells are produced from pre-existing cells.
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Who was Robert Hooke and what is his significance in cell biology?
Robert Hooke was the first person to view cells using a microscope and used the term 'cell' to describe these structures, marking the beginning of cell biology.
The idea that are made of cells is known as .
The idea that all living organisms are made of cells is known as cell theory.
What are the three main ideas of cell theory?
The three main ideas of cell theory are: all living organisms are made of one or more cells; cells are the basic functional unit of living organisms; and new cells are produced from pre-existing cells.
Unifying concept
A unifying concept in biology is an idea or theory that is universally accepted and helps to explain a wide range of biological phenomena, such as cell theory.
All cells share certain features, including a , cytoplasm, , and ribosomes.
All cells share certain features, including a cell surface membrane, cytoplasm, DNA, and ribosomes.
True or False?
Prokaryotic cells have internal membranes and larger ribosomes than eukaryotic cells.
False.
Prokaryotic cells have no internal membranes and smaller ribosomes compared to eukaryotic cells.
Which two scientists proposed the idea that all living organisms are made of cells?
Matthias Schleiden and Theodor Schwann proposed in 1837 that all living organisms are made of cells.
Ultrastructure
Ultrastructure refers to the detailed internal structures of a cell, which can be seen when the cell is examined under a microscope.
Cell theory
Cell theory states that cells are the basic functional unit of all living organisms.
Red blood cells are specialised to .
Red blood cells are specialised to transport oxygen.
What is a tissue in multicellular organisms?
A tissue is a group of cells of the same type that work together to perform a particular function.
Epithelial cells in the small intestine are specialised to efficiently.
Epithelial cells in the small intestine are specialised to absorb food efficiently.
True or False?
An organ is made up of only one type of tissue.
False.
An organ is made up of several different tissues working together to perform a particular function.
Organ system
An organ system is a group of organs working together to perform a particular function.
Xylem cells in plants are specialised to around a plant.
Xylem cells in plants are specialised to transport water around a plant.
What is the function of muscle tissue?
The function of muscle tissue is to contract to move parts of the body.
The is made up of the heart and blood vessels, and its function is to circulate blood around the body.
The circulatory system is made up of the heart and blood vessels, and its function is to circulate blood around the body.
Which tissues make up a leaf in plants?
A leaf in plants is made up of palisade mesophyll, spongy mesophyll, and vascular tissue.
Specialised cell
A specialised cell is a cell that has developed certain features to perform a specific function.
How do tissues, organs, and organ systems relate in multicellular organisms?
In multicellular organisms, specialised cells form tissues, tissues combine to form organs, and organs work together in organ systems.
Cell surface membrane
The cell surface membrane is the partially permeable outer boundary of the cell that controls the exchange of materials between the cell's internal and external environments.
What is the function of the nucleus in eukaryotic cells?
The nucleus contains genetic material (chromatin), is surrounded by the nuclear envelope, and controls the activities of the cell, including gene expression and ribosome production.
The nuclear envelope contains many that allow mRNA and ribosomes to exit the nucleus.
The nuclear envelope contains many nuclear pores that allow mRNA and ribosomes to exit the nucleus.
Mitochondria
Mitochondria are the double-membraned organelles responsible for aerobic respiration and ATP production in eukaryotic cells.
What is the function of ribosomes in cells?
Ribosomes are the sites of translation, where proteins are synthesised from mRNA using rRNA and proteins.
The endoplasmic reticulum is covered in ribosomes and involved in processing proteins.
The rough endoplasmic reticulum is covered in ribosomes and involved in processing proteins.
Golgi apparatus
The Golgi apparatus is a stack of flattened membrane sacs that modifies, packages, and transports proteins and lipids in vesicles.
What is the role of lysosomes in a cell?
Lysosomes contain hydrolytic enzymes that break down waste materials, worn-out organelles, and play a role in immune responses and apoptosis.
Centrioles are made from hollow fibres known as .
Centrioles are made from hollow fibres known as microtubules.
Eukaryotic cell
A eukaryotic cell is a type of cell with a membrane-bound nucleus and other organelles, found in animals, plants, fungi, and protists.
True or False?
Plant cells contain centrioles.
False.
Centrioles are found in animal cells but not in plant or fungal cells.
The cytoplasm of eukaryotic cells is divided into compartments called .
The cytoplasm of eukaryotic cells is divided into compartments called organelles.
What are three structural features unique to plant cells that are not found in animal cells?
The cytoplasm of eukaryotic cells is divided into compartments called organelles.
Eukaryotic cells are generally (larger/smaller) than prokaryotic cells.
Eukaryotic cells are generally larger than prokaryotic cells.
Ultrastructure
Ultrastructure refers to the detailed internal structure of a cell, as seen under an electron microscope.
What is the difference between 80S and 70S ribosomes?
80S ribosomes are found in eukaryotic cells, while 70S ribosomes are found in prokaryotes, mitochondria, and chloroplasts.
Organelle
An organelle is a specialised part of a cell that carries out a particular function.
Which cellular organelles are involved in the production and secretion of proteins?
The nucleus, ribosomes, rough endoplasmic reticulum (RER), Golgi apparatus and cell surface membrane are involved in the production and secretion of proteins.
Some organelles are -bound, meaning that they are surrounded by membrane.
Some organelles are membrane-bound, meaning that they are surrounded by membrane.
Where does transcription of the DNA code occur in a cell?
Transcription of the DNA code occurs in the nucleus.
Proteins formed within the cell are at the cell surface membrane.
Proteins formed within the cell are secreted at the cell surface membrane.
What is the main function of ribosomes on the RER?
Ribosomes on the rough endoplasmic reticulum (RER) produce proteins that can be secreted out of the cell or become attached to the cell surface membrane.
Processed proteins from the RER are transported to the apparatus in vesicles.
Processed proteins from the RER are transported to the Golgi apparatus in vesicles.
True or False?
The Golgi apparatus modifies proteins and prepares them for secretion from the cell.
True.
The Golgi apparatus modifies proteins and prepares them for secretion, such as in the case of extracellular enzymes.
Prokaryotic cell
A prokaryotic cell is a type of cell that lacks a membrane-bound nucleus and membrane-bound organelles, and has its genetic material as a single circular chromosome free in the cytoplasm.
Plasmid
A plasmid is a small, circular piece of DNA found in prokaryotic cells that is separate from the main bacterial chromosome and can replicate independently.
What is the function of the capsule in a prokaryotic cell?
The capsule protects the cell from desiccation and phagocytosis, and helps bacteria adhere to surfaces.
In prokaryotic cells, the cell wall is made of , also known as peptidoglycan.
In prokaryotic cells, the cell wall is made of murein, also known as peptidoglycan.
True or False?
All prokaryotic cells contain plasmids.
False.
Not all prokaryotic cells have plasmids; plasmids are only present in some prokaryotes.
Flagellum
A flagellum is a long, whip-like structure that enables movement of some prokaryotic cells.
Eukaryotic cells have 80S ribosomes, while prokaryotic cells have ribosomes.
Eukaryotic cells have 80S ribosomes, while prokaryotic cells have 70S ribosomes.
How is the genetic material of prokaryotic cells organised compared to eukaryotic cells?
In prokaryotic cells, genetic material is in a single circular chromosome free in the cytoplasm, not enclosed in a nucleus and not associated with proteins. In eukaryotic cells, DNA is linear, associated with proteins, and contained within a nucleus.
Pili
Pili are short, hair-like structures on the surface of some prokaryotic cells used for attachment to surfaces and for transferring genetic material between cells.
Prokaryotic cells have a that lacks membrane-bound organelles.
Prokaryotic cells have a cytoplasm that lacks membrane-bound organelles.
True or False?
The cell wall of prokaryotes is made of cellulose.
False.
The cell wall of prokaryotes is made of murein, not cellulose.
Which structural feature is found in all prokaryotic cells: capsule, plasmid, or ribosome?
All prokaryotic cells have ribosomes, but not all have capsules or plasmids.
Transmission Electron Microscope (TEM)
A Transmission Electron Microscope (TEM) uses a beam of electrons transmitted through a thin specimen to produce a high-resolution 2D image, allowing internal structures within cells or organelles to be seen.
Scanning Electron Microscope (SEM)
A Scanning Electron Microscope (SEM) produces 3D images by scanning a beam of electrons across the surface of a specimen, with the electrons bouncing off the surface and being detected.
What is the main difference between images produced by TEMs and SEMs?
TEMs produce high-resolution 2D images showing internal structures, while SEMs produce 3D images showing the surface of specimens.
TEMs use a beam of that is transmitted through a thin specimen to create a image.
TEMs use a beam of electrons that is transmitted through a thin specimen to create a 2D image.
SEMs can produce images that show the of specimens.
SEMs can produce 3D images that show the surface of specimens.
True or False?
TEM images have higher resolution than SEM images.
True.
TEM (Transmission Electron Microscope) images have a higher resolution than SEM (Scanning Electron Microscope) images, allowing finer internal details to be observed.
What type of electron microscope would you use to see the internal structure of an organelle in detail?
You would use a Transmission Electron Microscope (TEM) to view the internal structure of an organelle in detail.
SEMs do not require the specimen to be , whereas TEMs do.
SEMs do not require the specimen to be thin, whereas TEMs do.
Magnification
Magnification is how many times bigger the image of a specimen is compared to the actual size of the specimen.
Resolution (resolving power)
Resolution, or resolving power, is the ability to distinguish between two separate points in an image.
What is the formula for calculating total magnification using a light microscope?
Total magnification is calculated by multiplying the magnification of the eyepiece lens by the magnification of the objective lens.
The maximum resolution of a light microscope is . Any points separated by less than this distance cannot be .
The maximum resolution of a light microscope is 200 nm. Any points separated by less than this distance cannot be resolved.
True or False?
Electron microscopes can be used to observe living specimens.
False.
Electron microscopy requires the specimen to be dead.
Why are stains often used when preparing specimens for viewing with a light microscope?
Stains are used because the cytoplasm and cell structures are often transparent or difficult to distinguish without staining.
Differential staining
Differential staining is when multiple dyes are used so that different tissues within a specimen show up clearly.
Haematoxylin stains cell purple, brown, or blue, while methylene blue stains animal cell blue.
Haematoxylin stains cell nuclei purple, brown, or blue, while methylene blue stains animal cell nuclei blue.
What is one reason why the phospholipid bilayer of the cell membrane cannot be seen with a light microscope?
The phospholipid bilayer is about 10 nm thick, which is much smaller than the maximum resolution of a light microscope (200 nm).
Light microscopes use to view specimens that are typically than 200 nm, while electron microscopes use a beam of for higher resolution imaging.
Light microscopes use light to view specimens that are typically larger than 200 nm, while electron microscopes use a beam of electrons for higher resolution imaging.
Magnification
Magnification is how many times bigger the image of a specimen is compared to its real-life size.
What is the formula for calculating total magnification in a light microscope?
Total magnification = eyepiece lens magnification × objective lens magnification.
The equation for magnification is:
magnification = ÷
magnification = image size ÷ actual size
Why is it important to ensure that image size and actual size have the same units when calculating magnification?
It is important because using different units would result in an incorrect value for magnification; both values must be in the same units for the calculation to be accurate.
What is an eyepiece graticule?
An eyepiece graticule is a small disc with an engraved ruler placed in the microscope eyepiece to act as a ruler in the field of view.
How is the eyepiece graticule calibrated in a microscope?
The eyepiece graticule is calibrated using a stage micrometer, which has a known scale. The number of graticule divisions per micrometer unit is determined, so the size of each division can be calculated.
A stage micrometer is used to determine how many units each eyepiece graticule division represents.
A stage micrometer is used to determine how many micrometre units each eyepiece graticule division represents.
What is the main function of an optical (light) microscope in biology?
The main function of an optical microscope is to allow scientists to see and study tissues, cells, and organelles that are too small to be seen with the naked eye.
When preparing a microscope slide with a liquid specimen, a is gently pressed on top to remove air bubbles.
When preparing a microscope slide with a liquid specimen, a coverslip is gently pressed on top to remove air bubbles.
Why must tissue samples on slides be very thin when using a light microscope?
Tissue samples must be thin so that light can pass through and the structures can be seen clearly under the microscope.
What is a biological drawing?
A biological drawing is a line drawing made to record the observations seen under the microscope, following specific rules for clarity and accuracy.
True or False?
When drawing a tissue plan diagram, individual cells should be drawn in detail.
False.
In a plan diagram, only the arrangement of cells within a tissue or organ is shown; individual cell details are not drawn.
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