Exam code: 4SD0
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Why can't farmers control limiting factors for crops grown outside?
Growing crops outside leaves them exposed to natural conditions, so none of the limiting factors can be controlled.

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How do glasshouses help increase crop yields?
They provide an enclosed environment in which farmers can, to some extent, control the climate inside.
Name four conditions that can be manipulated in a glasshouse.
Artificial heating, so photosynthesis enzymes work faster
Artificial lighting, so plants photosynthesise for longer
Increased carbon dioxide in the air
Regular watering
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Why can't farmers control limiting factors for crops grown outside?
Growing crops outside leaves them exposed to natural conditions, so none of the limiting factors can be controlled.
How do glasshouses help increase crop yields?
They provide an enclosed environment in which farmers can, to some extent, control the climate inside.
Name four conditions that can be manipulated in a glasshouse.
Artificial heating, so photosynthesis enzymes work faster
Artificial lighting, so plants photosynthesise for longer
Increased carbon dioxide in the air
Regular watering
What must a farmer balance when using a glasshouse?
The extra cost of providing heating, lighting and carbon dioxide against the increased income from the higher yield.
Why must glasshouses in tropical countries be ventilated?
To release hot air and stop temperatures rising so high that the enzymes controlling photosynthesis denature.
What are polythene tunnels?
Large plastic tunnels that cover crops grown outside. They are more commonly called polytunnels.
Give three benefits of polythene tunnels.
They protect crops from the weather — excessive wind, rain and extreme temperatures
They increase the temperature slightly inside
They prevent the entry of pests and diseases
Why is artificial heating only used in temperate countries?
Because in tropical countries temperatures are already much hotter, so heating is unnecessary — the risk there is overheating.
Increasing the ________ content of glasshouse air lets plants photosynthesise more quickly.
Increasing the carbon dioxide content of glasshouse air lets plants photosynthesise more quickly.
Artificial ________ in a glasshouse allows plants to photosynthesise for longer each day.
Artificial lighting in a glasshouse allows plants to photosynthesise for longer each day.
True or False?
Polytunnels can protect crops from pests as well as weather.
True.
They prevent the entry of pests that damage plants and diseases that can kill them.
True or False?
A glasshouse should be kept as hot as possible.
False.
Too high a temperature denatures the enzymes controlling photosynthesis, so tropical glasshouses need ventilating.
Name the three main limiting factors for photosynthesis.
Temperature
Light intensity
Carbon dioxide concentration
Define limiting factor.
The factor in shortest supply at a given time, which restricts the rate of a process such as photosynthesis.
Why does increasing temperature raise the rate of photosynthesis?
Because the reaction is controlled by enzymes, which work faster as temperature rises.
Why does the rate fall if a glasshouse gets too hot?
Beyond a certain temperature the enzymes begin to denature, so the rate of reaction decreases.
Why does raising carbon dioxide increase crop yield?
Carbon dioxide is a raw material for photosynthesis, so the more present, the faster the reaction — until another factor becomes limiting.
How does light intensity affect the rate of photosynthesis?
The more light a plant receives, the faster the rate — until another required factor is in short supply.
What limits photosynthesis if light, CO₂, water and warmth are unlimited?
Only the plant's own ability to absorb these materials and make them react.
Raising the temperature increases photosynthesis because the reaction is controlled by ________.
Raising the temperature increases photosynthesis because the reaction is controlled by enzymes.
Above the optimum temperature the enzymes controlling photosynthesis begin to ________.
Above the optimum temperature the enzymes controlling photosynthesis begin to denature.
True or False?
Adding more carbon dioxide always increases the rate of photosynthesis.
False.
Only until another factor, such as light or temperature, becomes the limiting factor.
True or False?
Carbon dioxide is a raw material for photosynthesis.
True.
That is why increasing its concentration in a glasshouse can raise crop yields.
Why is a plant's rate of photosynthesis set by the lowest factor?
Because plants rarely have unlimited supplies of light, carbon dioxide, water and warmth, so whichever is lowest restricts the rate.
How do fertilisers increase crop yield?
They increase the key nutrients in the soil, so crop plants grow larger and healthier, and faster and bigger — raising yields.
Why must mineral ions be replaced in farmland soil?
Because crop plants take mineral ions up from the soil, so they must be replaced if crops are grown in the same field year after year.
Give two examples of organic fertiliser.
Farmyard manure
Compost
How are chemical fertilisers applied?
As dry granules spread on the soil, or sprayed on in liquid form.
What are magnesium ions needed for, and what shows a deficiency?
Needed for making chlorophyll.
A deficiency causes yellowing of the leaves.
What are nitrate ions needed for, and what shows a deficiency?
Needed for making amino acids, the building blocks of proteins.
A deficiency causes weak growth and yellowing of the leaves.
Which two mineral ions do fertilisers supply to crops?
Magnesium ions — for making chlorophyll
Nitrate ions — for making amino acids
What is the drawback of applying too much fertiliser?
The excess is leached into rivers and lakes, where it causes eutrophication — so more fertiliser does not always mean a better outcome.
Magnesium ions are needed by a plant to make ________.
Magnesium ions are needed by a plant to make chlorophyll.
Farmyard manure and compost are examples of ________ fertilisers.
Farmyard manure and compost are examples of organic fertilisers.
True or False?
Yellow leaves can indicate either a magnesium or a nitrate deficiency.
True.
Both cause yellowing of the leaves, though nitrate deficiency also causes weak growth.
True or False?
Plants absorb nitrogen gas from the air to make amino acids.
False.
Plants take up nitrate ions from the soil — not nitrogen gas.
Define pesticide.
A chemical that kills unwanted species, reducing damage to crops and increasing yields.
Give three ways pests reduce crop yields.
Insects and other animals damage crops by eating them
Weeds compete with crops for space, water and nutrients
Fungi infect crops and spread diseases
Name the three types of pesticide and what each kills.
Insecticides — kill insect pests
Herbicides — kill weed species
Fungicides — kill fungal pests
Give three advantages of using pesticides.
Easily accessible and relatively cheap
Have an immediate effect
Can kill the entire population of pests
Give four disadvantages of using pesticides.
Pests can develop resistance to them
They are non-specific and can kill beneficial organisms, such as pollinating bees
They can be persistent and bioaccumulate in top predators
They must be repeatedly applied
Define bioaccumulation.
When a persistent chemical does not break down in the body and builds up in great concentrations at the top of food chains, harming top predators.
Define biological control.
Using a natural predator to eat a pest species, reducing the pest's impact on crop yields.
Give two examples of biological control.
Ladybirds eating aphids, which happens naturally
Parasitic wasps introduced to control whitefly in glasshouse tomato crops
Why can't biological control remove a pest completely?
Because it is based on a predator-prey cycle, so it keeps the pest at lower levels rather than eliminating it.
Give four advantages of biological control.
A natural method, so no pollution
No resistance develops
Can target specific species
Long lasting, and does not need repeated application
Give four disadvantages of biological control.
May eat other organisms instead of the pest
Takes longer to be effective
Cannot kill the entire population
May not adapt, may move away, or may become a pest itself
Chemicals that kill weed species are called ________.
Chemicals that kill weed species are called herbicides.
Persistent pesticides can build up in top predators, a process called ________.
Persistent pesticides can build up in top predators, a process called bioaccumulation.
True or False?
Pests can become resistant to biological control.
False.
No resistance develops with biological control — that is one of its advantages over pesticides.
True or False?
Insecticides can harm bees as well as pest insects.
True.
Pesticides are non-specific, so they can kill beneficial organisms such as the bees that pollinate crops.
What kind of organism is yeast?
A single-celled fungus that can carry out both aerobic and anaerobic respiration.
How does yeast obtain sugars from bread dough?
It produces enzymes that break down the starch in flour, releasing sugars it can use in respiration.
When does yeast in dough switch to anaerobic respiration?
It respires aerobically at first, then switches to anaerobic respiration when the oxygen runs out.
What does yeast produce during anaerobic respiration?
Alcohol (ethanol) and carbon dioxide.
Why does bread dough rise?
The carbon dioxide produced by the yeast is trapped in small air pockets in the dough, increasing its volume.
Why does baked bread contain no alcohol?
Any ethanol produced by the yeast is evaporated by the heat during baking.
What happens to the yeast during baking?
It is killed by the high temperatures, so there is no further respiration.
Give the word equation for anaerobic respiration in yeast.
glucose → ethanol + carbon dioxide
Bread dough rises because ________ produced by the yeast is trapped in the dough.
Bread dough rises because carbon dioxide produced by the yeast is trapped in the dough.
Yeast is a single-celled ________ that can respire with or without oxygen.
Yeast is a single-celled fungus that can respire with or without oxygen.
True or False?
Yeast can only respire anaerobically.
False.
Yeast can carry out both aerobic and anaerobic respiration, switching to anaerobic when oxygen runs out.
True or False?
The yeast survives the baking process.
False.
The high temperatures kill the yeast, which stops any further respiration.
What does yeast produce when it respires anaerobically?
Ethanol and carbon dioxide. The process is called fermentation.
Outline the method for this investigation.
Mix yeast with sugar solution in a boiling tube, to provide glucose.
Add a layer of oil on top.
Connect the tube by a delivery tube to another boiling tube of limewater.
Place in a water bath at a set temperature and count the bubbles produced in a fixed time, such as 2 minutes.
Repeat at different temperatures.
Why is a layer of oil added on top of the yeast solution?
To prevent oxygen entering the solution, so the yeast cannot respire aerobically.
How is the rate of anaerobic respiration measured?
By the rate at which carbon dioxide is produced — counting the bubbles given off in a fixed time.
What is the limewater for in this investigation?
It shows that the gas being produced is carbon dioxide, by turning cloudy.
Why do more bubbles appear as the temperature rises?
The temperature moves closer to the optimum for the yeast's enzymes, increasing enzyme activity and so the rate of respiration.
What happens if the temperature goes above the optimum?
The enzymes denature, so carbon dioxide production slows down and eventually stops.
Which variables must be controlled in this investigation?
The concentration, volume and pH of the sugar solution
The mass of yeast added
The species of yeast used
Why is fermentation economically important?
It is used in the manufacture of bread, where carbon dioxide makes the dough rise, and of alcoholic drinks, since ethanol is a type of alcohol.
Anaerobic respiration in yeast cells is called ________.
Anaerobic respiration in yeast cells is called fermentation.
A layer of ________ on the yeast solution stops oxygen getting in.
A layer of oil on the yeast solution stops oxygen getting in.
True or False?
Respiration in yeast is an enzyme-controlled reaction.
True.
That is why the rate rises with temperature towards the optimum and then falls as the enzymes denature.
True or False?
Raising the temperature always speeds up yeast fermentation.
False.
Only up to the optimum temperature. Above it, the enzymes denature and the rate falls.
Which bacterium is used to make yoghurt?
Lactobacillus.
Give two reasons bacteria are useful in food production.
They can produce complex molecules, such as the enzymes that turn milk into yoghurt
They reproduce rapidly, so the amount of chemical they produce increases quickly
Outline the process of making yoghurt.
Sterilise all equipment.
Pasteurise the milk at 85–95 °C.
Cool it to 40–45 °C and add Lactobacillus.
Incubate for several hours while the bacteria digest milk proteins and ferment the lactose.
Cool to 5 °C, then stir and add any flavourings before packaging.
Why is the milk pasteurised before the bacteria are added?
To kill other, unwanted bacteria, which could otherwise compete for the lactose and spoil the taste of the yoghurt.
What does Lactobacillus do to the lactose in milk?
It converts the lactose into lactic acid.
How does lactic acid turn milk into yoghurt?
The increased acidity sours and thickens the milk, forming yoghurt.
How does the lower pH act as a preservative?
It prevents the growth of other, possibly harmful microorganisms, so the yoghurt keeps longer than fresh milk.
Why is the yoghurt cooled to 5 °C at the end?
To halt the action of the *Lactobacillus* bacteria.
At what temperature is the Lactobacillus added, and why?
40–45 °C — warm enough for the bacteria to work quickly, but not hot enough to kill them.
Lactobacillus converts the lactose in milk into ________.
Lactobacillus converts the lactose in milk into lactic acid.
Before the bacteria are added, the milk is ________ at 85–95 °C to kill unwanted bacteria.
Before the bacteria are added, the milk is pasteurised at 85–95 °C to kill unwanted bacteria.
True or False?
Yoghurt keeps for longer than fresh milk.
True.
The lowered pH prevents the growth of other microorganisms, acting as a preservative.
True or False?
Contaminating bacteria would speed up yoghurt production.
False.
They would slow it down by competing with the *Lactobacillus* for the lactose, and could spoil the taste.
Define fermenter.
A container used to grow (culture) microorganisms such as bacteria and fungi in large amounts.
Give four uses of an industrial fermenter.
Making yoghurt
Making mycoprotein
Producing genetically modified bacteria
Growing moulds that produce antibiotics, such as penicillin
What is the main advantage of using a fermenter?
Conditions can be carefully controlled, producing large quantities of exactly the right type of microorganism.
Why are aseptic precautions needed in a fermenter?
The fermenter is cleaned by steam to kill microorganisms and prevent chemical contamination, so that only the desired microorganism grows.
Why are nutrients supplied in a fermenter?
They are used in respiration to release energy for growth, and ensure the microorganisms can reproduce.
How and why is temperature controlled in a fermenter?
It is monitored with probes and maintained using a water jacket.
This keeps enzymes at their optimum — too hot and they denature, too cold and they work too slowly.
How is pH controlled in a fermenter?
It is monitored with a probe and adjusted if necessary using acids or alkalis, to keep it at the optimum for that microorganism.
Why is oxygenation needed in a fermenter?
So that aerobic respiration can take place.
What is the purpose of agitation in a fermenter?
Stirring paddles ensure microorganisms, nutrients, oxygen, temperature and pH are evenly distributed throughout the fermenter.
Temperature in a fermenter is maintained using a ________ around the vessel.
Temperature in a fermenter is maintained using a water jacket around the vessel.
A fermenter is cleaned with ________ to kill unwanted microorganisms.
A fermenter is cleaned with steam to kill unwanted microorganisms.
True or False?
Stirring paddles help distribute heat evenly through a fermenter.
True.
Agitation evenly distributes microorganisms, nutrients, oxygen, temperature and pH.
True or False?
Fermenters are only used to make food products.
False.
They are also used for non-food processes, such as producing genetically modified bacteria and antibiotic-producing moulds.
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