Nitrogen Cycle (Edexcel IGCSE Biology): Revision Note

Exam code: 4BI1

Lára Marie McIvor

Written by: Lára Marie McIvor

Reviewed by: Lucy Kirkham

Updated on

The nitrogen cycle

  • Nitrogen is present as N2 gas in the atmosphere and within biological molecules, such as proteins, in the tissues of living organisms

  • Nitrogen is cycled through ecosystems via the nitrogen cycle

Nitrogen fixation

  • Nitrogen fixation makes nitrogen available to living organisms

  • Nitrogen fixing bacteria convert N2 gas into ammonium compounds, which are then converted into nitrates in the soil

  • Nitrogen fixing bacteria include:

    • Free-living bacteria in the soil

    • Bacteria in the root nodules of leguminous plants (e.g. peas, beans, clover) which form a mutualistic relationship with the plant

  • Nitrogen can also be fixed by lightning or during the production of chemical fertilisers

  • After nitrogen fixation has occurred plants absorb nitrates from the soil to build plant proteins

Transfer of nitrogen between living organisms

  • Animals feed on plants, digest the proteins in the plant tissues, providing nitrogen to build animal proteins

  • Nitrogen may then be passed from one consumer to another up the food chain in the same way

Release of nitrogen from tissues

  • Nitrogen from dead organisms and metabolic waste products is returned to the soil as ammonia by decomposers (bacteria and fungi)

    • Ammonia reacts with water in the soil to form ammonium ions

  • Plants can’t absorb ammonium ions directly, so nitrifying bacteria convert ammonia into nitrites, and then into nitrates, which can be taken up again by plants

  • The conversion of ammonium compounds to nitrates is known as nitrification, and can be summarised as follows:

ammonia → nitrites → nitrates

Denitrification

  • Denitrifying bacteria convert nitrates back into N₂ gas, returning nitrogen to the atmosphere. This process is known as denitrification

  • Denitrifying bacteria are active in anaerobic conditions, e.g. in waterlogged or compacted soil

  • Farmers reduce denitrification by ploughing the soil to increase aeration

Diagram illustrating the nitrogen cycle, showing processes like nitrogen fixation, nitrification, denitrification, and the role of plants, animals, and bacteria.

The nitrogen cycle involves nitrogen fixation, decomposition, nitrification and denitrification

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Lára Marie McIvor

Author: Lára Marie McIvor

Expertise: Content Creator

Lára graduated from Oxford University in Biological Sciences and has now been a science tutor working in the UK for several years. Lára has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning.

Lucy Kirkham

Reviewer: Lucy Kirkham

Expertise: Content Creator

Lucy has been a passionate Maths teacher for over 12 years, teaching maths across the UK and abroad helping to engage, interest and develop confidence in the subject at all levels.Working as a Head of Department and then Director of Maths, Lucy has advised schools and academy trusts in both Scotland and the East Midlands, where her role was to support and coach teachers to improve Maths teaching for all.