Exam code: H420
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Surface area to volume (SA:V) ratio
The ratio comparing an organism's total surface area to its total volume. It decreases as an organism increases in size.

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Why can single-celled organisms rely on simple diffusion to exchange substances?
They have a high SA:V ratio:
The large surface area allows maximum absorption of nutrients and gases and secretion of waste products
The small volume means the diffusion distance to all organelles is short
What happens to an organism's SA:V ratio as it increases in size?
Its SA:V ratio decreases.
This means there is less surface area for exchange relative to volume, and a longer diffusion distance to the cells and tissues.
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Surface area to volume (SA:V) ratio
The ratio comparing an organism's total surface area to its total volume. It decreases as an organism increases in size.
Why can single-celled organisms rely on simple diffusion to exchange substances?
They have a high SA:V ratio:
The large surface area allows maximum absorption of nutrients and gases and secretion of waste products
The small volume means the diffusion distance to all organelles is short
What happens to an organism's SA:V ratio as it increases in size?
Its SA:V ratio decreases.
This means there is less surface area for exchange relative to volume, and a longer diffusion distance to the cells and tissues.
Why do large multicellular organisms need specialised exchange surfaces and transport systems?
Their low SA:V ratio and larger size mean:
There is insufficient surface area to absorb enough nutrients and gases and to remove waste by diffusion alone
The diffusion distance to internal cells and tissues is too long, so diffusion would be too slow
As organisms increase in size, their surface area to volume ratio .
As organisms increase in size, their surface area to volume ratio decreases.
Give examples of specialised systems that large multicellular animals and plants have evolved for exchange and transport.
Gas exchange system
Circulatory system
Lymphatic system
Urinary system
Xylem and phloem (in plants)
Why do organisms need a supply of oxygen delivered to their cells?
Cells require ATP to carry out the biochemical processes needed for survival.
The majority of ATP is produced through aerobic respiration, which requires oxygen.
Why must carbon dioxide be removed from cells and tissues?
Carbon dioxide is a toxic waste product of aerobic respiration.
If it accumulates in cells/tissues it alters the pH.
Using diffusion time, explain why diffusion works for a single-celled organism like Chlamydomonas but not for a large organism.
In Chlamydomonas (diameter 20 μm), oxygen only needs to diffuse a maximum of 10 μm to reach the centre, which is very quick.
Over a much larger diffusion distance the diffusion time increases substantially, becoming far too long to supply the tissues of a large organism.
Metabolic rate
The amount of energy expended by an organism within a given period of time.
How can the metabolic rate of an organism be measured or estimated?
Oxygen consumption (using a respirometer)
Carbon dioxide production (using a carbon dioxide probe)
Heat generation (using a calorimeter)
Explain why small animals need a relatively high metabolic rate to maintain their body temperature.
Heat is lost to the environment at the body's surface.
Small animals have a higher SA:V ratio, so they lose more heat to their surroundings.
To replace this heat and maintain body temperature, they need a relatively high metabolic rate.
How does the metabolic rate per unit body mass compare between small and large animals?
Smaller animals have a higher metabolic rate per unit of body mass than larger animals.
However, larger organisms support more cells overall, so they still consume more oxygen in total within a given period of time.
Why do plants generally have much lower metabolic rates than animals?
They do not move around their habitat and do not have to maintain a high body temperature.
True or False: As an organism increases in size, its surface area to volume ratio increases.
False — as an organism increases in size, its SA:V ratio decreases.
True or False: Smaller animals have a higher metabolic rate per unit of body mass than larger animals.
True
What are the four features of an effective exchange surface?
Large surface area
Short diffusion distance (thin)
Good blood supply
Ventilation mechanism
Why is simple diffusion an efficient exchange mechanism for a single-celled organism such as Chlamydomonas?
It is very small, so it has a large surface area to volume ratio and a short diffusion distance to the centre of the cell.
This means oxygen can diffuse across the cell surface membrane quickly enough to meet the cell's needs, without a specialised exchange surface.
How are root hair cells adapted for the absorption of water and mineral ions?
They have a root hair (an extension of the cytoplasm) that increases the surface area of the cell in contact with the soil.
The larger surface area increases the rate of water uptake by osmosis and allows more mineral ions to be absorbed.
How is the wall of an alveolus adapted to provide a short diffusion distance?
The walls of the alveoli are only one cell thick and these cells are flattened.
This gives gases a very short diffusion distance, so gas exchange is quick and efficient.
How does having a large number of alveoli aid gas exchange in the lungs?
It greatly increases the surface area available for oxygen and carbon dioxide to diffuse across, increasing the rate of gas exchange.
How does an extensive capillary network around the alveoli help maintain the concentration gradient for gas exchange?
The constant flow of blood carries oxygenated blood away from the alveoli and brings deoxygenated blood to them.
This maintains the concentration gradient needed for oxygen and carbon dioxide to continue diffusing.
How does a good (adequate) blood supply help maintain a concentration gradient at an exchange surface?
The blood is continuously flowing, carrying away substances that have just entered the blood from the exchange site.
This keeps the concentration difference high, so diffusion continues for a prolonged period of time.
Counter-current system (fish gills)
An arrangement in the gills where blood flows in the opposite direction to the flow of water. This maintains the concentration gradient for oxygen diffusion along the whole length of the capillary/gill.
Explain how the counter-current system in fish gills makes gas exchange efficient.
Blood flows in the opposite direction to the flow of water over the gills.
This maintains a concentration gradient along the whole length of the capillary, so oxygen diffuses from the water into the blood along the entire gill.
Ventilation
The mass flow of gases (the movements of breathing) that refreshes the air at an exchange surface, helping to maintain the concentration gradient across it.
Explain how a ventilation mechanism maintains the concentration gradient in the alveoli.
Breathing constantly changes the air in the alveoli
It removes air with a low oxygen and high carbon dioxide concentration and replaces it with air high in oxygen and low in carbon dioxide
This ensures there is always a higher oxygen concentration in the alveoli than in the blood, maintaining the gradient
The walls of the alveoli are only one thick, giving gases a short diffusion distance.
The walls of the alveoli are only one cell thick, giving gases a short diffusion distance.
In fish gills, blood flows in the opposite direction to the water in a system.
In fish gills, blood flows in the opposite direction to the water in a counter-current system.
True or False: The walls of the alveoli are many cells thick.
False — the walls of the alveoli are only one cell thick, giving a short diffusion distance.
True or False: A counter-current system maintains the concentration gradient along the whole length of the fish gill.
True
Thorax
The chest cavity: a collection of organs and tissues where gas exchange takes place in mammals.
What is the function of cartilage in the gas exchange system?
It is a strong, flexible tissue that forms rings supporting the trachea (and bronchi), keeping the airway open while still allowing it to move and flex during breathing.
How does ciliated epithelium help protect the lungs?
Each cell has small projections of cilia that sweep mucus, dust and bacteria upwards, away from the lungs and the epithelium itself.
What is the role of goblet cells in the gas exchange system?
They are mucus-producing cells that secrete viscous mucus.
The mucus traps dust, bacteria and other microorganisms, preventing them from reaching the lungs.
The trapped material is swept upwards by cilia, swallowed, and destroyed by acid in the stomach.
Why is the squamous epithelium lining the alveoli well adapted for gas exchange?
It forms the alveolar wall and is very thin and permeable, allowing the easy, rapid diffusion of gases.
Where is smooth muscle found in the gas exchange system?
It is found in the walls of the bronchi and bronchioles.
Why are elastic fibres important in lung tissue?
Elastic fibres enable the lungs to stretch and then recoil.
Elastic fibres allow the lungs to stretch and then , making expiration a passive process.
Elastic fibres allow the lungs to stretch and then recoil, making expiration a passive process.
Describe the role of the capillary network surrounding each alveolus.
Carbon dioxide diffuses out of the capillaries into the alveoli to be exhaled.
Oxygen diffuses the other way, from the alveoli into the capillaries, to be carried around the body.
The capillaries are narrow enough for only one red blood cell to pass at a time, ensuring sufficient time and opportunity for gas exchange.
Why are the cartilage rings of the trachea C-shaped?
The C-shape prevents friction from rubbing against the oesophagus located close behind, while still keeping the air channel open at all times.
How does the structure of the bronchi compare with that of the trachea?
Bronchi have a similar structure to the trachea but with thinner walls and a smaller diameter.
Their cartilage does not form a C-shape; it can form full rings or irregular blocks.
How do bronchioles differ from the trachea and bronchi in structure?
They are narrow, self-supporting tubes with thin walls
They are not usually supported by cartilage
They are lined with ciliated epithelium but usually do not contain goblet cells
Larger bronchioles contain elastic fibres and smooth muscle to adjust airflow; the smallest have elastic fibres but no smooth muscle
Describe the structure of an alveolus that adapts it for gas exchange.
The wall consists of a single layer of (squamous) epithelium
Elastic fibres are present in the extracellular matrix
It is surrounded by an extensive capillary network
A watery fluid lines the alveoli, facilitating the diffusion of gases
Goblet cells and mucous glands produce mucus in the trachea, which is swept upwards by the cilia of the epithelium.
Goblet cells and mucous glands produce mucus in the trachea, which is swept upwards by the cilia of the ciliated epithelium.
What is the function of smooth muscle in the gas exchange system?
It regulates the flow of air into the lungs by dilating when more air is needed and constricting when less air is needed.
How do elastic fibres make expiration a passive process?
Their recoil pushes air out of the lungs, so expiration requires no active muscular effort.
True or False: Bronchioles are usually supported by rings of cartilage.
False — bronchioles are narrow, self-supporting tubes that are not usually supported by cartilage.
True or False: The capillaries around each alveolus are wide enough for only one red blood cell to pass at a time.
True
Ventilation
The mass flow of gases into and out of the lungs (breathing), which continually refreshes the air in the alveoli.
Why is ventilation important for efficient gas exchange in the lungs?
Ventilation, together with the continuous flow of blood in the capillaries, ensures there is always a higher concentration of oxygen in the alveoli than in the blood.
This maintains a steep concentration gradient for oxygen to diffuse from the alveoli into the blood.
How do the movements of breathing help maintain gas exchange in the alveoli?
The movements of breathing change the air in the alveoli, which:
supplies fresh oxygen to the alveoli
removes carbon dioxide from the alveoli
List, in order, the passage of air from outside the body to the site of gas exchange.
Nose / mouth
Trachea (windpipe)
Bronchi
Bronchioles
Alveoli
What changes in chest volume and lung pressure cause air to move into the lungs during breathing in?
The volume in the chest increases
The air pressure in the lungs decreases until it is slightly lower than atmospheric pressure
Air then moves down the pressure gradient and rushes into the lungs
Describe the mechanism of breathing in (inhalation) when at rest.
The diaphragm contracts and flattens, increasing the volume of the chest.
This decreases the pressure in the lungs, so air is drawn in.
During exercise, what additional muscle action assists breathing in?
In addition to the diaphragm flattening, the external intercostal muscles contract, causing the ribcage to move upwards and outwards.
This further increases chest volume, drawing in more air.
What changes in chest volume and lung pressure cause air to leave the lungs during breathing out?
The volume in the chest decreases
The pressure in the lungs increases
Air is forced out of the lungs
Describe the mechanism of breathing out (exhalation) when at rest.
The external intercostal muscles relax
The recoil of elastic fibres surrounding the alveoli forces air out
The diaphragm relaxes and becomes dome-shaped
During exercise, how is forced exhalation brought about?
The internal intercostal muscles contract to pull the ribs downwards and inwards
The abdominal muscles contract to push the organs upwards against the diaphragm, increasing the internal pressure
This produces a forced exhalation.
What is meant by the intercostal muscles working in an antagonistic manner?
As one set of intercostal muscles contracts, the other set relaxes.
The external and internal intercostal muscles work against each other to move the ribcage up (breathing in) and down (breathing out).
During inhalation at rest, the diaphragm contracts and , increasing the volume of the chest.
During inhalation at rest, the diaphragm contracts and flattens, increasing the volume of the chest.
How does increased ventilation help meet the body's needs during exercise?
Exercise causes oxygen demand to increase.
An increased rate of ventilation supplies oxygen and removes carbon dioxide more quickly, helping to meet this higher demand.
Inspiration / expiration
Alternative terms for breathing in and breathing out. Inspiration (= inhalation) is breathing in; expiration (= exhalation) is breathing out.
True or False: During inhalation, the diaphragm contracts and flattens.
True — a flattened diaphragm increases chest volume and lowers lung pressure, drawing air in.
True or False: During normal exhalation at rest, air is forced out mainly by the intercostal and diaphragm muscles contracting.
False — at rest these muscles relax; air is forced out mainly by the elastic recoil of the lungs.
Vital capacity
The maximum volume of air that can be breathed in or out in one breath.
Tidal volume
The volume of air breathed in or out during normal breathing (at rest).
Breathing rate
The number of breaths taken in one minute (one breath = taking air in and breathing it back out again).
Oxygen uptake
The volume of oxygen used up by someone in a given time.
Residual volume
The small amount of air always retained in the lungs after as much air as possible has been breathed out.
What are the four main aspects of breathing that can be measured scientifically?
Vital capacity
Tidal volume
Breathing rate
Oxygen uptake
What is a spirometer used for?
A spirometer is a piece of apparatus used to measure different aspects of breathing.
The subject breathes in and out through it, allowing their vital capacity, tidal volume, breathing rate and oxygen uptake to be calculated.
Why is soda lime included in a spirometer?
Soda lime absorbs carbon dioxide from the exhaled air.
This stops the concentration of carbon dioxide in the re-breathed air from getting too high, which could otherwise cause respiratory distress.
How does a spirometer record the subject's breathing?
As the subject breathes, a trace is drawn on a rotating drum of paper, or a graph is formed digitally and viewed on a computer.
From this, vital capacity, tidal volume and breathing rate can be calculated.
Explain how a spirometer is used to measure oxygen uptake.
As the subject breathes, carbon dioxide is removed from the exhaled air by soda lime.
Oxygen is continually extracted from the air by the subject's breathing, so the total volume of air in the spirometer gradually decreases.
This change in volume is used as a measure of oxygen uptake.
How do you calculate breathing rate from a spirometer trace?
Count the number of breaths in 60 seconds (one breath = the trace going up and then down)
Express the value in the correct units: breaths min⁻¹
For example, 12 up-and-down movements in the first 60 seconds = 12 breaths min⁻¹.
How do you calculate tidal volume from a spirometer trace?
Measure the difference between the top and bottom of the trace for a single breath, in terms of the volume of air in the lungs.
For example, at rest: 3 dm³ − 2.6 dm³ = 0.4 dm³.
Carbon dioxide is absorbed from the exhaled air in a spirometer by .
Carbon dioxide is absorbed from the exhaled air in a spirometer by soda lime.
is the volume of air always retained in the lungs after as much air as possible has been breathed out.
Residual volume is the volume of air always retained in the lungs after as much air as possible has been breathed out.
True or False: The soda lime in a spirometer absorbs oxygen from the exhaled air.
False — soda lime absorbs carbon dioxide, not oxygen.
True or False: As a subject breathes into a spirometer, the total volume of air inside gradually decreases.
True
Spiracles
Openings in the exoskeleton of an insect that allow air to flow into the internal system of tubes (the tracheal system).
Tracheae (singular: trachea)
Tubes within the insect respiratory system that carry air inwards and lead to narrower tubes called tracheoles.
Why is the insect exoskeleton unable to act as a gas exchange surface?
It is rigid and has a waxy coating that makes it impermeable to gases, so gases cannot diffuse across it.
How is the structure of the tracheal system adapted for efficient gas exchange in insects?
Rigid rings of chitin keep the tracheae open
Many tracheoles carry oxygen directly to the muscle fibres, where gas exchange takes place
The large number of tracheoles in contact with the cells provides a large surface area for gas exchange
What is the role of the tracheal fluid at the ends of the tracheoles?
Gases dissolve in the tracheal fluid before diffusing to the cells, allowing gas exchange to take place.
How do insects obtain enough oxygen when at rest?
At rest, energy requirements are low, so diffusion alone is fast enough to supply oxygen to the cells.
How does an active insect increase its supply of oxygen using body movements?
Contracting and relaxing the muscles of the thorax and abdomen alters the volume, and therefore the pressure, inside the tracheae, drawing air in and out.
During flight, how is the diffusion distance in the tracheoles reduced?
The tracheal fluid at the narrow ends of the tracheoles is drawn into the respiring muscle.
Removing the fluid reduces the diffusion distance between the air and the muscle cells, speeding up diffusion.
Why do fish need specialised gas exchange surfaces rather than relying on gas exchange across the general body surface like some insects?
Oxygen dissolves less readily in water; a given volume of air contains about 30 times more oxygen than the same volume of water.
Fish gills are adapted to directly extract this smaller amount of oxygen from water.
Describe the structure of the gills of a bony fish, from gill arch to lamellae.
A series of gills sits on each side of the head
Each gill arch is attached to two stacks of filaments
On the surface of each filament are rows of lamellae
Each lamella is covered by a single layer of flattened cells over a vast network of capillaries
Counter-current system (in fish gills)
An arrangement in which blood in the lamellae flows in the opposite direction to the flow of water, maintaining the concentration gradient along the whole length of the capillary so oxygen diffuses into the blood along the entire lamella.
In the counter-current system, the water with the lowest oxygen concentration is found adjacent to the most blood.
In the counter-current system, the water with the lowest oxygen concentration is found adjacent to the most deoxygenated blood.
How does a fish draw water into its buccal cavity?
The fish opens its mouth and lowers the floor of the buccal cavity.
This increases the volume and so decreases the pressure within the cavity.
Pressure is higher outside the mouth, so water flows into the buccal cavity.
How does water move across the gills and leave the fish during ventilation?
The fish raises the floor of the buccal cavity, closing its mouth and increasing the pressure inside.
Water flows from the buccal cavity (high pressure) into the gill cavity (low pressure).
Pressure builds in the gill cavity, forcing the operculum open so water exits the fish.
Operculum
A flap of tissue covering the gills of a bony fish; it is forced open to let water leave and is pulled shut when the floor of the buccal cavity is lowered at the start of the next ventilation cycle.
How do insects reduce water loss when at rest?
They may close their spiracles to reduce water loss by evaporation.
True or False: A given volume of air contains about 30 times more oxygen than the same volume of water.
True
True or False: In a fish gill counter-current system, blood and water flow in the same direction.
False — blood flows in the opposite direction to the water, which maintains the concentration gradient along the whole lamella.
Why are dissections a valuable part of scientific research?
They allow the internal structures of organs to be examined, so that theories can be made about how those organs function.
State two ethical concerns surrounding the use of animals for dissection.
Concerns about how the animals are raised and killed
It goes against the religious beliefs of some individuals
What should be considered about the source and disposal of a biological specimen used for dissection?
The specimen should be obtained from a reputable source and disposed of correctly.
When dissecting multiple specimens for comparison, what should be true of the specimens?
They should be taken from individual organisms of the same species and of roughly the same age.
Why should a lab coat, gloves and eye protection be worn during a dissection?
To avoid contamination with biological material, which could otherwise cause an allergic reaction.
Compare the use of scissors and a scalpel in a dissection.
Scissors: used for cutting large sections of tissue, where cuts do not need to be precise
Scalpel: enables finer, more precise cutting and needs to be sharp to ensure this
State two safety precautions to follow when using a scalpel during a dissection.
Cut away from your body
Keep your fingers far from the blade to reduce the chance of cutting yourself
During a dissection, what are pins used for?
To move sections of the specimen aside, leaving the desired structure exposed.
State three limitations of using dissection to examine gas exchange surfaces.
It can be hard to see the smaller, finer structures within organs
The specimens do not reflect how tissue looks in a living organism
With only a single specimen, anomalies may be ignored or glossed over
Which key structures can be seen from a dissection of bony fish gills?
Gill arch
Filaments
The smaller lamellae can be hard to distinguish in a dissected fish.
Which key structures can be seen from a dissection of mammalian lungs?
Trachea
Bronchi
Bronchioles
The smaller alveoli can be hard to distinguish in a dissected lung.
Why is it difficult to examine the gas exchange system of an insect by dissection?
The insect tracheal system is very small, so specialised equipment and skills are sometimes required to dissect it, and microscopes are needed to observe the structures.
In a photomicrograph of stained mammalian lung tissue, the alveoli appear as different sizes and shapes because they are no longer as they would be in a living lung.
In a photomicrograph of stained mammalian lung tissue, the alveoli appear as different sizes and shapes because they are no longer inflated as they would be in a living lung.
Why can gas exchange surfaces look very different in a photomicrograph compared with a textbook diagram?
Real tissue often appears very different from idealised textbook diagrams, so it is important to be able to identify the gas exchange surface and its key structures.
What can be used to take clear images of an insect's spiracle?
Electron microscopes can take clear images of the spiracle structures found on the surface of insects.
True or False: In a dissected bony fish gill, the lamellae are easily distinguished.
False — the smaller lamellae can be hard to distinguish in a dissected fish.
True or False: Dissected tissue accurately reflects how tissue appears in a living organism.
False — specimens do not reflect how tissue looks in a living organism.
Spiracle
An external opening on the surface of an insect through which air enters the tracheal system.
Gill arch
The structure that resembles a backbone for the gills, supporting the filaments.
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