Exam code: H420
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Ecosystem
A relatively self-contained community of interacting organisms and the environment they live in, and interact with.

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What two broad categories of components make up an ecosystem?
Living (biotic) components – e.g. plants, animals, bacteria, fungi
Non-living (abiotic) components – e.g. soil, water, rocks, physical and chemical factors
What happens to energy and nutrients within an ecosystem?
There is a flow of energy through the ecosystem, and the nutrients within it are recycled.
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Ecosystem
A relatively self-contained community of interacting organisms and the environment they live in, and interact with.
What two broad categories of components make up an ecosystem?
Living (biotic) components – e.g. plants, animals, bacteria, fungi
Non-living (abiotic) components – e.g. soil, water, rocks, physical and chemical factors
What happens to energy and nutrients within an ecosystem?
There is a flow of energy through the ecosystem, and the nutrients within it are recycled.
How do individual species relate to communities and ecosystems?
Species do not exist in isolation; they interact with other species to form communities.
These communities interact with each other and with the environment they live in, forming ecosystems.
Explain why no ecosystem is completely self-contained.
Organisms from one ecosystem are often linked to organisms from another.
For example, birds can fly long distances to feed from multiple ecosystems.
Give examples to show that ecosystems vary greatly in size and scale.
A small pond in a back garden
The open ocean
An individual human being (thousands of species of bacteria live on and in every person)
How do ecosystems differ in complexity?
Ecosystems vary in complexity, ranging from relatively simple to highly complex.
Biotic factor
Anything that influences the populations within a community as a result of another organism's activity.
Abiotic factor
Any physical or chemical (non-living) factor that influences the populations within a community.
Give five examples of biotic factors that can influence a community.
Predation
Competition (inter-specific) for space, food, water, light etc.
Cooperation between organisms
Parasitism
Disease
Camouflage
Mimicry
Mankind (e.g. habitat loss, hunting, farming)
Using mimicry as a biotic factor, explain how a hoverfly benefits from resembling a wasp.
A hoverfly is harmless, yet has evolved body colouring like that of a wasp.
This deters potential predators, which behave as though it could deliver a harmful sting, so the hoverfly is less likely to be eaten.
Give six examples of abiotic factors that can influence a community.
Availability of water
Light
Temperature
Humidity
pH
Salinity
Soil composition
Atmospheric composition
Turbidity of water
A biotic factor arises from another organism's activity, whereas an factor is a physical or chemical, non-living influence on a community.
A biotic factor arises from another organism's activity, whereas an abiotic factor is a physical or chemical, non-living influence on a community.
Why should you be precise when describing 'water' as an abiotic factor affecting aquatic organisms?
'Water' covers several distinct aspects, each of which can affect organisms differently, so you should specify which you mean, e.g.:
Salinity
Flow rate
Mineral content
Turbidity
Depth
Dissolved oxygen concentration
Give an example of a simple ecosystem and a complex ecosystem.
Simple: a desert
Complex: a tropical rainforest
True or False: No ecosystem is completely self-contained.
True — organisms from one ecosystem are often linked to those in another, e.g. birds feeding across multiple ecosystems.
True or False: Within an ecosystem, nutrients flow through and are lost, while energy is recycled.
False — energy flows through an ecosystem, while nutrients are recycled.
Biomass
The mass of living material in an organism or tissue, and the chemical energy stored within that organism or tissue.
In what three ways can biomass be measured?
The dry mass of an organism or tissue in a given area
The mass of carbon the organism or tissue contains
The chemical energy content released when the sample is burned in pure oxygen
Dry mass
The mass of an organism or tissue after all the water has been removed (i.e. after it has been fully dehydrated).
The mass of carbon that a sample contains is generally taken to be of the dry mass of the sample.
The mass of carbon that a sample contains is generally taken to be 50% of the dry mass of the sample.
Why is the dry mass of a sample used to measure biomass rather than its fresh (wet) mass?
The water content of an organism or tissue can vary and does not represent living material or stored chemical energy. Removing all the water gives a reliable, comparable measure of the actual living material present.
How can the dry mass of a single organism be used to estimate the biomass of a larger population or area?
Multiply the dry mass of one organism (or of a given sample area) by the total number of organisms (or the total area).
For example, if one daffodil has a dry mass of 0.1 kg, then 200 daffodils have a biomass of 0.1 x 200 = 20 kg.
Why is biomass sometimes given with units of time (e.g. kg m⁻² yr⁻¹)?
Because biomass can change over time (for example, deciduous trees lose biomass over autumn and winter as they shed leaves). Including a time unit shows the average biomass within a given area over that time period.
Calorimetry
A technique used to estimate the chemical energy stored in dry biomass by burning the sample and measuring the heat released.
How does a calorimeter estimate the chemical energy stored in a dried sample of biomass?
The dried sample is burned inside the calorimeter
The heat released is used to warm a known volume of water
The change in temperature of the water is measured
This temperature change is used to estimate the chemical energy stored in the sample (measured in joules or kilojoules)
Describe the method for finding the dry mass of a plant sample.
Weigh the crucible without the sample
Place the sample in the crucible and put it in an oven set to a low temperature (to avoid burning the sample)
Remove and weigh the crucible and sample at regular intervals
Continue until the mass stops decreasing and becomes constant (the sample is now fully dehydrated)
Subtract the original mass of the empty crucible from this final constant mass to find the dry mass
Why must the oven be set to a low temperature when drying a plant sample to find its dry mass?
If the temperature is too high, the sample may burn, which would cause it to lose biomass and give an inaccurate dry mass measurement.
How do you know when a plant sample has been fully dehydrated during dry mass measurement?
When the mass of the crucible and sample stops decreasing and becomes constant, all the water has been removed and the sample is fully dehydrated.
Give the main limitations of finding the dry mass and energy value of plant biomass.
Drying a sample fully can take a long time (several days), because it must be heated at a relatively low temperature to avoid burning
Precise equipment (e.g. a highly precise balance and thermometer) is needed and may not be available
A simple, basic calorimeter gives a less accurate energy estimate because heat is lost and not transferred efficiently to the water
Why does a bomb calorimeter give a more accurate estimate of chemical energy than a simple calorimeter?
A bomb calorimeter ensures that almost all the heat energy from the burning sample is transferred to the water (minimal heat loss), giving a highly accurate estimate of the chemical energy content.
True or False: The mass of carbon in a sample is generally taken to be 50% of its dry mass.
True
True or False: A simple, basic calorimeter gives a more accurate energy estimate than a bomb calorimeter.
False — a bomb calorimeter is more accurate because almost all the heat is transferred to the water, with minimal heat loss.
Give three reasons why a large proportion of the Sun's energy is not made available to photosynthetic plants.
Light falls away from plants (misses them)
Light passes through leaves or is reflected away
Only certain wavelengths of light stimulate photosynthesis
During photosynthesis, what energy conversion do primary producers such as plants and algae carry out?
They convert light energy into chemical energy, which is stored in biological molecules.
The storing of chemical energy as plant makes a certain amount of energy available to the next trophic level.
The storing of chemical energy as plant biomass makes a certain amount of energy available to the next trophic level.
Explain why only a small percentage of plant biomass becomes biomass in the primary consumer.
Not all of the plant's biomass is eaten by the primary consumer
Not all of the consumer's biomass intake is digested (e.g. cellulose is lost in faeces)
The consumer converts much of the chemical energy to movement and heat, leaving only a small amount for new biomass
Approximately what is the typical efficiency of biomass transfer from one trophic level to the next?
The efficiency is low, typically around 10%.
State the formula for calculating the percentage efficiency of biomass transfer between trophic levels.
Efficiency of transfer = (biomass transferred ÷ biomass intake) × 100
Where:
Biomass transferred = biomass passed to the higher trophic level
Biomass intake = biomass of the lower trophic level that has been consumed
A blackberry bush of mass 35 kg is fed upon by aphids with a collective mass of 4.1 kg. Calculate the percentage efficiency of energy transfer for this step.
Step 1: Check units match
Both values are in kg, so no conversion is needed.
Step 2: Substitute into the formula
(4.1 ÷ 35) × 100 = 11.7%
Net primary productivity (NPP)
The net productivity of producers: the chemical energy stored as biomass that is available to the next trophic level, calculated as NPP = GPP − R (gross primary productivity minus respiratory losses).
State the equation used to calculate the net productivity of consumers, defining each term.
N = I − (F + R)
Where:
I = chemical energy store in ingested food
F = chemical energy lost to the environment in faeces and urine
R = respiratory losses to the environment
Toads ingest 10,000 kJ m⁻² yr⁻¹ of energy, losing 2,000 kJ m⁻² yr⁻¹ in faeces and urine and 7,000 kJ m⁻² yr⁻¹ in respiration. Calculate the percentage efficiency of energy transfer from insects to toads.
Step 1: Calculate net productivity of the toads
N = I − (F + R) = 10,000 − (2,000 + 7,000) = 1,000 kJ m⁻² yr⁻¹
Step 2: Calculate % efficiency
% Efficiency = (1,000 ÷ 10,000) × 100 = 10%
Describe methods an arable (crop) farmer can use to increase the efficiency of biomass transfer to producers.
Providing artificial light in greenhouses on overcast days
Optimising planting distances between crops
Irrigation in dry weather and use of fertilisers
Selective breeding for fast growth
Use of fungicides/pesticides and herbicides to reduce competition/pests
Fencing to exclude grazers
Describe methods a livestock farmer can use to increase the efficiency of biomass transfer to primary consumers.
Use of good quality feeds / food supplements
Use of antibiotics and vaccines to reduce disease
Controlling predation with fencing or indoor husbandry
Reducing competition for grazing (e.g. rabbits, deer)
Indoor husbandry to reduce energy loss from movement or from getting cold
Why can exam questions refer to biomass and energy interchangeably?
Because the biomass of an organism is effectively a measure of how much chemical energy is stored within it.
True or False: The efficiency of biomass transfer between trophic levels is typically around 10%.
True
True or False: All of the biomass a primary consumer ingests is converted into new biomass in its own body.
False — much chemical energy is lost as movement and heat, and undigested biomass is lost in faeces.
Gross primary productivity (GPP)
The total chemical energy that producers fix as biomass through photosynthesis, before respiratory losses are subtracted (NPP = GPP − R).
Saprobiont
A true decomposer (mainly from the fungi and bacteria kingdoms) that obtains nutrients from dead or waste organic matter by secreting enzymes onto it and digesting it externally.
Saprobiotic nutrition
The method of obtaining nutrients from dead or waste organic matter via extracellular digestion, followed by absorption of the soluble products.
Describe how saprobionts digest their food.
They secrete enzymes onto their food (dead organisms and waste material)
The material is digested externally (extracellular digestion)
The soluble products are then absorbed by the saprobiont
Why are saprobionts an essential component of ecosystems?
They recycle the nutrients locked up in dead and waste matter, making them available to producers again.
Without them, nutrients would remain locked in dead/waste matter and producers such as plants would not have access to sufficient nutrients.
Give two examples of mineral ions released during saprobiotic nutrition that are important for producers.
Ammonium ions
Phosphate ions
(Not all products are absorbed by saprobionts — many remain in the soil and are available to other organisms such as plants.)
Mycorrhizae
The symbiotic (mutually beneficial) relationships between fungi and the roots of plants.
How do mycorrhizae benefit plants?
Fungal filaments (hyphae) greatly increase the surface area of the roots
This increases absorption of water and mineral ions (e.g. nitrates and phosphates)
The fungi in a mycorrhiza are composed of long, thin filaments known as , which interact with the roots of the plant.
The fungi in a mycorrhiza are composed of long, thin filaments known as hyphae, which interact with the roots of the plant.
What is the role of nitrogen-fixing bacteria in the nitrogen cycle?
They convert gaseous nitrogen (from the atmosphere) into ammonium ions (NH₄⁺).
Examples: Azotobacter and Rhizobium species.
What is the role of nitrifying bacteria in the nitrogen cycle?
They nitrify ammonium ions into nitrite (NO₂⁻) and then nitrate (NO₃⁻), which are highly soluble and can be absorbed by plant roots.
Examples: Nitrosomonas and Nitrobacter species.
What is the role of denitrifying bacteria in the nitrogen cycle?
They use nitrates for respiration and return gaseous nitrogen to the atmosphere.
Example: Thiobacillus denitrificans.
Ammonium ions are converted into nitrite and nitrate by bacteria in a process called nitrification.
Ammonium ions are converted into nitrite and nitrate by nitrifying bacteria in a process called nitrification.
Why is atmospheric nitrogen not directly usable by most organisms?
Nitrogen is the most abundant atmospheric gas but is relatively inert (unreactive), so most organisms cannot use it directly.
True or False: Nitrogen is the most abundant gas in the atmosphere but is relatively inert.
True
True or False: Nitrifying bacteria convert gaseous nitrogen from the atmosphere into ammonium ions.
False — nitrogen-fixing bacteria convert gaseous nitrogen into ammonium ions; nitrifying bacteria convert ammonium ions into nitrite and nitrate.
Why is the number of carbon atoms in the biosphere essentially constant?
Carbon is constantly recycled around the biosphere.
Carbon atoms are not created or destroyed; they merely swap from one compound to another via the various processes of the carbon cycle.
State the main forms in which carbon is stored in the biosphere.
In the atmosphere (as CO₂)
In sedimentary rocks
In fossil fuels (coal, oil and gas; coal is almost pure carbon)
In soil and other organic matter
In vegetation (e.g. as cellulose)
Dissolved in the oceans (as CO₂)
Autotroph
An organism that uses the energy of sunlight to 'fix' carbon dioxide, converting its carbon into sugars and other organic molecules.
How does photosynthesis affect atmospheric carbon dioxide?
Autotrophs use the energy of sunlight to 'fix' CO₂, turning its carbon into sugars and other organic molecules.
This removes carbon from the atmosphere.
In which part of photosynthesis is CO₂ fixed?
CO₂ is fixed in the Calvin cycle.
Compare the source of CO₂ used by terrestrial plants and by aquatic organisms in photosynthesis.
Terrestrial plants use gaseous CO₂ directly from the air
Aquatic organisms use CO₂ dissolved in water
As much CO₂ is fixed from ocean microorganisms as from terrestrial plants.
How does sedimentation lock carbon away?
Dead plants are not fully decomposed by saprobionts; their bodies form layers of sediment that accumulate over millions of years, locking carbon into the ground.
How does respiration contribute to the carbon cycle?
All organisms respire, including autotrophs, and heterotrophs rely on respiration for all their energy needs.
Respiration releases CO₂ into the atmosphere, the opposite direction to photosynthesis.
In aerobic respiration, at which stages is CO₂ released?
CO₂ is released in the Link Reaction and the Krebs Cycle.
Anaerobic respiration also releases CO₂ into the atmosphere, via by yeast, moulds and bacteria.
Anaerobic respiration also releases CO₂ into the atmosphere, via fermentation by yeast, moulds and bacteria.
How is carbon passed along a food chain during feeding?
Carbon is passed from autotroph to heterotroph, and from primary consumer to secondary consumer, during feeding.
Biomass transfer always includes the transfer of carbon, as it is the main element in biomass.
How do decay and decomposition return carbon to the surroundings?
Dead plants and animals are fed upon by detritivores and decayed by saprophytes, which:
Releases carbon into the surroundings
Supplies carbon to the detritivores and saprophytes
Waste matter such as faeces and urine is also used by decaying saprobionts, and these processes can release CO₂ back into the air.
Explain how burning fossil fuels has disturbed the carbon cycle.
Since the mid-19th century, humans have extracted and burned increasing amounts of fossil fuels.
CO₂ is returned to the atmosphere faster than it can be absorbed by plants and aquatic producers. Warmer temperatures also mean less CO₂ dissolves in the oceans, so more is released into the air, driving climate change and changing many species' habitats.
How is CO₂ fixed within the Calvin cycle?
The enzyme Rubisco catalyses the fixation, by carboxylating RuBP.
What can the carbon locked away by sedimentation go on to form?
It can form fossil fuels such as peat and coal.
Dead aquatic organisms form sediments on the sea bed that can form oil and gas, while shells and other calcium-containing parts form sedimentary rocks such as limestone.
True or False: Respiration and photosynthesis move carbon in the same direction.
False — respiration releases CO₂ into the atmosphere whereas photosynthesis removes it, so they act in opposite directions.
True or False: Atmospheric CO₂ is now roughly double what it was 800,000 years ago.
True
Succession
The process by which an ecosystem changes over time, typically from a very simple community to a relatively complex one, as both the biotic (living) and abiotic (non-living) conditions change.
Why are ecosystems described as 'dynamic'?
Because they are constantly changing over time, with both biotic and abiotic conditions altering as succession proceeds.
Primary succession
The process that occurs when newly formed or newly exposed land, with no species initially present, is gradually colonised by an increasing number of species.
Give two ways in which new, uninhabited land for primary succession can be created.
Magma from erupting volcanoes cools to form new rock surfaces or rocky islands\n\n- A drop in sea level or the drying up of a lake exposes areas of bare rock
Pioneer species
The first species to colonise newly formed or exposed land (often mosses and lichens), which are adapted to survive in hostile abiotic conditions.
Why are marram grasses well adapted to act as pioneer species on coastal sand dunes?
They have deep roots to reach water other plants cannot access\n\n- They can tolerate the salty environment (high concentrations of sodium and calcium ions from sea spray)
Describe the stages of primary succession on a newly exposed rock surface.
Wind-carried seeds and spores land on the rock; pioneer species (moss and lichens) grow\n\n- Pioneers die and decompose, forming humus and a basic soil\n\n- Small plants and grasses colonise the shallow, nutrient-poor soil\n\n- These die and decompose, making the soil deeper and more nutrient-rich; roots help hold the soil in place\n\n- Larger plants, shrubs and small trees grow in the deeper soil\n\n- Finally, the soil supports large trees, which become the dominant species of the climax community
Climax community
The final, stable community formed at the end of succession, containing all the plant and animal species that have colonised the land, dominated by the largest species the environment can support (e.g. large trees).
As pioneer species die and decompose, the dead organic matter, known as , forms a basic soil.
As pioneer species die and decompose, the dead organic matter, known as humus, forms a basic soil.
How do pioneer species such as lichens change the environment to make it more suitable for other species?
They help to break apart the top surface of the rock, and when they die they leave behind humus. Together the fragmented rock and humus form a basic soil, making conditions less hostile so species such as mosses can colonise.
Why does one species often replace the previous species during succession?
Each colonising species changes the local environment so that it becomes less suitable for the previous species. For example, once a thin soil layer covers the rock, lichens can no longer grow and disappear; later, trees may out-compete shrubs by blocking light.
Deflected succession
When human activities (or other factors) prevent or interrupt succession, stopping a climax community from developing.
Give two examples of how human or animal activity can halt succession in a grassy area.
Regular mowing prevents larger, woody plants from establishing, so only grasses survive\n\n- Grazing by livestock (sheep, horses, cattle) removes new plant shoots, so grasses remain the dominant plants
Describe two methods used to deliberately prevent succession for conservation purposes.
Introducing grazing animals temporarily, so they eat the shoots of shrubs and trees and stop them establishing\n\n- Managed (controlled) burning, which burns away shrubs and trees; species such as heather regrow quickly, resetting succession
Why can deliberately preventing an ecosystem from reaching its climax community be beneficial for conservation?
Intermediate stages of succession often hold a distinct diversity of plant species of conservation importance, which provide food and habitat for a high diversity of animal species (e.g. rare species or important pollinators). These would be lost if the climax community developed. Maintaining both climax and earlier-stage areas gives a higher overall species diversity.
True or False: Primary succession begins on land where no species were previously present.
True
True or False: Lichens can continue to grow once a thin layer of soil completely covers the rock surface.
False — lichens cannot grow on soil, so they disappear from the ecosystem once soil covers the rock.
Why is sampling used to investigate the biodiversity of large, complex ecosystems such as rainforests?
It is impossible to find, identify and count every organism in large, complex ecosystems, and cataloguing every species would be extremely time-consuming.
Instead, samples of the area are taken and used to make an estimate of total species numbers.
Distribution (of a species)
How a species is spread throughout an ecosystem.
Abundance (of a species)
The number of individuals of that species in an area.
Describe the difference between random and systematic sampling.
In random sampling, the positions of the sampling points are completely random (due to chance), which avoids sampling bias.
In systematic sampling, the positions of the sampling points are deliberately chosen by the person carrying out the sampling.
When is random sampling the most appropriate choice?
When the area is reasonably uniform or has no clear pattern to the distribution of species.
When is systematic sampling more appropriate?
When there are changes in physical (abiotic) conditions across the area, e.g. changes in altitude, soil pH or light intensity.
What is a key advantage of using random sampling?
It removes sampling bias from the person carrying out the sampling, so the results are more representative of the whole area.
State the three main sampling methods used to estimate population size, and the type of organism each is suited to.
Quadrats — for non-motile or slow-moving species
Transects — for non-motile or slow-moving species
Mark-release-recapture — for motile species
How can a quadrat be placed randomly to avoid sampling bias?
Convert the sampling area into a grid and label each square with a number
Use a random number generator to select the sample points (co-ordinates)
Lay the quadrat at each chosen point and record the abundance of the species present
What is species frequency?
Species frequency is the probability that a species will be found within any quadrat in the sample area.
How is species frequency calculated from quadrat data?
Divide the number of quadrats the species was present in by the total number of quadrats, then multiply by 100.
E.g. bluebells found in 18 of 50 quadrats: (18 ÷ 50) × 100 = 36%.
What is percentage cover?
Percentage cover is an estimate of the proportion of a quadrat occupied by a species. The quadrat is divided into 100 smaller squares, and the number of squares the species is found in gives its percentage cover.
E.g. grass in 89 of 100 squares = 89% cover.
When is percentage cover used instead of counting individuals?
When it is difficult to count individual plants or organisms.
Describe how a belt transect is used to investigate species distribution.
Lay a measuring tape in a line across the area (e.g. where abiotic factors change)
Place quadrats at regular intervals along the tape
Record the abundance of each species within each quadrat
This produces quantitative data showing how distribution changes with physical conditions.
Describe the mark-release-recapture method for estimating the population size of a motile species.
Take a first large sample; count and mark individuals in a way that won't affect survival
Release the marked individuals and allow them to mix randomly with the population
After sufficient time, take a second large sample and count the marked and unmarked individuals
Use the proportion of marked to unmarked individuals to estimate the total population size
State the mark-release-recapture formula for estimating population size (N) and define each term.
N = (n₁ × n₂) ÷ m₂
N = population estimate
n₁ = number of marked individuals released
n₂ = number of individuals in the second sample (marked and unmarked)
m₂ = number of marked individuals in the second sample
What are the main assumptions made when using the mark-release-recapture method?
Marked individuals have sufficient time to disperse and mix back into the population
The marking does not affect survival rates (e.g. does not increase predation)
The marking remains visible and does not rub off
The population size stays constant — no significant births, deaths or migration during the study
The scale (Abundant, Common, Frequent, Occasional, Rare) is used by biologists to estimate abundance in habitats that are difficult to sample quantitatively, such as lichens on tree branches.
The ACFOR scale (Abundant, Common, Frequent, Occasional, Rare) is used by biologists to estimate abundance in habitats that are difficult to sample quantitatively, such as lichens on tree branches.
True or False: Mark-release-recapture is the most suitable method for estimating the population size of non-motile species such as plants.
False — mark-release-recapture is used for motile species; quadrats and transects are used for non-motile species.
True or False: Random sampling helps to remove sampling bias from the person carrying out the investigation.
True
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