Exam code: 8465
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Define resolving power.
Resolving power is the ability of a microscope to distinguish between two points that are close together. A higher resolving power means finer detail can be seen.

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What is the maximum useful magnification of a light microscope?
The maximum useful magnification of a light microscope is about ×1000. Beyond this, images become blurry rather than revealing more detail.
True or False?
Electron microscopes can magnify objects up to ×1 000 000 or more.
True.
Electron microscopes can magnify up to ×1 000 000 or more, far exceeding the ~×1000 limit of light microscopes.
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Define resolving power.
Resolving power is the ability of a microscope to distinguish between two points that are close together. A higher resolving power means finer detail can be seen.
What is the maximum useful magnification of a light microscope?
The maximum useful magnification of a light microscope is about ×1000. Beyond this, images become blurry rather than revealing more detail.
True or False?
Electron microscopes can magnify objects up to ×1 000 000 or more.
True.
Electron microscopes can magnify up to ×1 000 000 or more, far exceeding the ~×1000 limit of light microscopes.
To convert from millimetres (mm) to micrometres (µm), you multiply by .......... .
To convert from millimetres (mm) to micrometres (µm), you multiply by 1000.
Why can electron microscopes reveal subcellular structures that light microscopes cannot?
Electron microscopes can reveal subcellular structures that light microscopes cannot because they have a much higher resolving power. This allows structures such as mitochondria and the cell membrane to be studied in fine detail.
True or False?
The magnification equation is: magnification = image size × actual size.
False.
The correct equation is magnification = image size ÷ actual size. Multiplying the two values does not give a meaningful result.
Define magnification.
Magnification is how many times larger an image is compared to the actual object. It is calculated as image size divided by actual size, and has no units.
A cell image measures 30 mm and the actual cell is 0.01 mm wide. What is the magnification?
The magnification is ×3000. Using the equation magnification = image size ÷ actual size: 30 ÷ 0.01 = 3000. Magnification has no units.
Complete the unit conversion table.
Conversion | Multiply or divide by |
|---|---|
mm → µm | |
µm → nm | |
cm → mm |
Complete the unit conversion table.
Conversion | Multiply or divide by |
|---|---|
mm → µm | × 1000 |
µm → nm | × 1000 |
cm → mm | × 10 |
Define eukaryotic cell.
A eukaryotic cell is a cell whose genetic material is enclosed within a nucleus. Both animal and plant cells are eukaryotic cells.
What subcellular structures are found in animal cells but NOT in plant cells?
No structures are exclusive to animal cells. Animal cells contain a nucleus, cytoplasm, cell membrane, mitochondria, and ribosomes — all of which plant cells also have.
True or False?
Prokaryotic cells have their DNA enclosed in a nucleus.
False.
In prokaryotic cells, the DNA is not enclosed in a nucleus. Instead, it exists as a single loop of DNA in the cytoplasm.
Complete the table to show which structures are present in animal and plant cells.
Structure | Animal cell | Plant cell |
|---|---|---|
Nucleus | ✓ | |
Chloroplasts | ✓ (some) | |
Cell wall | ||
Mitochondria | ✓ | |
Permanent vacuole |
Complete the table to show which structures are present in animal and plant cells.
Structure | Animal cell | Plant cell |
|---|---|---|
Nucleus | ✓ | ✓ |
Chloroplasts | ✗ | ✓ (some) |
Cell wall | ✗ | ✓ |
Mitochondria | ✓ | ✓ |
Permanent vacuole | ✗ | ✓ |
What is the function of mitochondria?
The function of mitochondria is to carry out aerobic respiration, releasing energy for the cell to use in its activities.
Define cell wall.
A cell wall is a rigid outer layer found in plant and algal cells, made of cellulose. It strengthens the cell and helps it maintain its shape.
True or False?
The permanent vacuole in plant cells is filled with cell sap.
True.
The permanent vacuole is a large fluid-filled space found in plant cells. It is filled with cell sap and helps to keep the cell rigid.
Prokaryotic cells have their DNA in the .......... as a single loop, and may also contain smaller circular pieces of DNA called .......... .
Prokaryotic cells have their DNA in the cytoplasm as a single loop, and may also contain smaller circular pieces of DNA called plasmids.
Why do only some plant cells contain chloroplasts?
Only some plant cells contain chloroplasts because only cells exposed to light need to carry out photosynthesis. Cells in roots, for example, are not exposed to light and do not require chloroplasts.
Define temporary mount.
A temporary mount is a slide prepared for short-term viewing under a microscope. A thin specimen is placed on a glass slide with a drop of water and covered with a coverslip.
Why is a stain used when preparing a microscope slide?
A stain is used when preparing a microscope slide to make cells or cell structures more visible. Many cell components are colourless, so stains such as iodine or methylene blue add contrast.
True or False?
You should start with the highest power objective lens when first focusing a light microscope.
False.
You should always start with the lowest power objective lens. This makes it easier to locate the specimen before switching to higher magnification for more detail.
When making a temporary mount, the specimen is placed on a glass .........., a drop of water is added, and it is covered with a .......... .
When making a temporary mount, the specimen is placed on a glass slide, a drop of water is added, and it is covered with a coverslip.
How do you calculate the total magnification of a microscope?
The total magnification of a microscope is calculated by multiplying the magnification of the eyepiece lens by the magnification of the objective lens. For example, ×10 eyepiece × ×40 objective = ×400 total.
True or False?
Onion skin cells need to be stained with iodine to be seen clearly under a light microscope.
True.
Onion skin cells are largely colourless, so staining with iodine solution colours the cells and makes the nucleus and other structures visible under the microscope.
Complete the table showing steps for preparing a microscope slide.
Step | Action |
|---|---|
1 | Add a drop of water to the centre of the slide |
2 | |
3 | Lower a coverslip at an angle to avoid air bubbles |
4 | |
5 | View under the microscope, starting with the lowest power lens |
Complete the table showing steps for preparing a microscope slide.
Step | Action |
|---|---|
1 | Add a drop of water to the centre of the slide |
2 | Place the specimen on the slide |
3 | Lower a coverslip at an angle to avoid air bubbles |
4 | Add a drop of stain if needed |
5 | View under the microscope, starting with the lowest power lens |
Why is it important to lower the objective lens carefully when first focusing?
It is important to lower the objective lens carefully when first focusing to avoid cracking the slide or damaging the lens. The lens should be lowered while looking from the side, then raised slowly while looking through the eyepiece.
Define diffusion.
Diffusion is the net movement of particles from an area of higher concentration to an area of lower concentration, due to the random movement of particles in a solution or gas.
True or False?
Diffusion is a passive process.
True.
Diffusion does not require energy from the cell. The particles move using their own kinetic energy, so diffusion is entirely passive.
The three factors that affect the rate of diffusion are the .........., the .......... and the .......... .
The three factors that affect the rate of diffusion are the concentration gradient, the temperature and the surface area.
Why does increasing temperature increase the rate of diffusion?
At higher temperatures, particles have more kinetic energy. This means they move faster, so the net movement from high to low concentration happens more quickly.
Define osmosis.
Osmosis is the diffusion of water molecules from a dilute solution to a more concentrated solution through a partially permeable membrane.
True or False?
Active transport moves substances down a concentration gradient.
False.
Active transport moves substances against a concentration gradient, from a more dilute solution to a more concentrated solution. This requires energy from respiration.
What is a partially permeable membrane?
A partially permeable membrane is a membrane that allows small molecules such as water to pass through, but does not allow larger molecules through.
In plants, root hair cells use active transport to absorb .......... ions from the dilute solution in the soil.
In plants, root hair cells use active transport to absorb mineral ions from the dilute solution in the soil.
Give two examples of active transport in living organisms.
Root hair cells absorb mineral ions from dilute solutions in the soil into the cell
Sugar molecules are absorbed from the gut into the blood when the blood already has a higher sugar concentration
Transport processes across cell membranes:
Process | Direction of movement | Energy required? |
|---|---|---|
Diffusion | ||
Osmosis | ||
Active transport |
Transport processes across cell membranes:
Process | Direction of movement | Energy required? |
|---|---|---|
Diffusion | High to low concentration | No |
Osmosis | Dilute to concentrated solution | No |
Active transport | Low to high concentration | Yes |
Define osmosis.
Osmosis is the diffusion of water molecules through a partially permeable membrane, from a region of higher water concentration (dilute solution) to a region of lower water concentration (concentrated solution).
What is the independent variable in the osmosis required practical?
The independent variable is the concentration of the salt or sugar solution (measured in mol/dm³). Different concentrations are tested to see how they affect the potato tissue.
True or False?
Potato cylinders gain mass when placed in a concentrated salt solution.
False.
In a concentrated solution, the water concentration outside the potato is lower than inside, so water moves out of the potato by osmosis, causing it to lose mass.
Percentage change in mass = ((final mass − .......... mass) ÷ .......... mass) × 100
Percentage change in mass = ((final mass − initial mass) ÷ initial mass) × 100
What does it mean if the line on an osmosis graph crosses the x-axis?
When the line crosses the x-axis, the percentage change in mass is zero. This means the concentration of the solution matches the concentration inside the potato, so no net movement of water occurs by osmosis.
True or False?
Temperature is a control variable in the osmosis required practical.
True.
Temperature must be kept constant to ensure it is a fair test. Temperature affects the rate of osmosis, so any change would affect the results.
Define partially permeable membrane.
A partially permeable membrane is a membrane that allows small molecules such as water to pass through, but blocks larger molecules. Cell membranes act as partially permeable membranes.
Complete the variables table for the osmosis required practical.
Variable type | Example |
|---|---|
Independent | |
Dependent | |
Control | Temperature, time, type of tissue |
Complete the variables table for the osmosis required practical.
Variable type | Example |
|---|---|
Independent | Concentration of solution |
Dependent | Percentage change in mass of potato |
Control | Temperature, time, type of tissue |
Why is percentage change in mass used rather than just the change in mass?
Percentage change in mass is used rather than just the change in mass because it accounts for differences in the starting mass of each potato cylinder. This allows for fair comparison between samples.
Define chromosomes.
Chromosomes are thread-like structures found in the nucleus of cells, made from coiled DNA. In human body cells, chromosomes are found in 23 pairs (46 in total).
How many chromosomes are in a typical human body cell?
A typical human body cell contains 46 chromosomes, arranged in 23 pairs. One chromosome in each pair is inherited from each parent.
True or False?
The two daughter cells produced by mitosis are genetically identical to each other.
True.
Mitosis produces two daughter cells that are genetically identical to each other and to the original parent cell. The DNA is copied exactly before the cell divides.
During the cell cycle, the .......... material is copied and the cell then divides to form .......... new cells that are genetically identical.
During the cell cycle, the genetic material is copied and the cell then divides to form two new cells that are genetically identical.
What must a cell do before it can divide by mitosis?
Before a cell can divide by mitosis, it must:
Grow larger
Make copies of all its organelles (e.g. mitochondria, ribosomes)
Replicate all chromosomes in the nucleus
True or False?
Human body cells contain 23 chromosomes.
False.
Human body cells contain 46 chromosomes (23 pairs). Only gametes (sex cells) contain 23 chromosomes — one from each pair.
Define mitosis.
Mitosis is a type of cell division that produces two genetically identical daughter cells. It is used for growth and repair of tissues in multicellular organisms.
Complete the table describing what happens during mitosis.
Event | Description |
|---|---|
Chromosome sets | The two complete sets are pulled to |
New nuclei | |
Final step | The cell splits into two |
Result |
Complete the table describing what happens during mitosis.
Event | Description |
|---|---|
Chromosome sets | The two complete sets are pulled to opposite sides of the cell |
New nuclei | Two new nuclei form |
Final step | The cell splits into two |
Result | Two genetically identical daughter cells |
Why is mitosis important in multicellular organisms?
Mitosis is important in multicellular organisms because it enables growth during development and the repair of damaged tissues. It produces new cells that are genetically identical to the original.
Define gametes.
Gametes are sex cells produced in the reproductive organs. In humans, they are the egg (female) and sperm (male) cells. Each gamete contains only one set of chromosomes (23 in humans).
How many cells does meiosis produce, and how many chromosomes does each contain?
Meiosis produces four daughter cells. Each contains a single set of chromosomes (23 in humans), which is half the number found in a normal body cell.
True or False?
Cells produced by meiosis are all genetically identical to each other.
False.
Cells produced by meiosis are all genetically different from each other. This genetic variation is important for natural selection and the survival of species.
When a cell divides by meiosis, it first copies its .......... information, then divides .......... to produce four gametes.
When a cell divides by meiosis, it first copies its genetic information, then divides twice to produce four gametes.
Why must chromosome number be halved during the formation of gametes?
The chromosome number must be halved so that when two gametes fuse at fertilisation, the resulting zygote has the correct number of chromosomes. Without halving, the chromosome number would double with each generation.
True or False?
The zygote formed at fertilisation divides by mitosis to grow.
True.
After fertilisation, the zygote divides repeatedly by mitosis to form an embryo, and eventually all the tissues and organs of the offspring.
Define zygote.
A zygote is a fertilised egg cell, formed when a male and female gamete fuse. It contains the full number of chromosomes (46 in humans), half from each parent.
Complete the comparison table for mitosis and meiosis.
Feature | Mitosis | Meiosis |
|---|---|---|
Purpose | Growth and repair | |
Number of divisions | Two | |
Daughter cells produced | Two | |
Genetic variation | None |
Complete the comparison table for mitosis and meiosis.
Feature | Mitosis | Meiosis |
|---|---|---|
Purpose | Growth and repair | Production of gametes |
Number of divisions | One | Two |
Daughter cells produced | Two | Four |
Genetic variation | None | Yes — all cells differ |
Where in the body does meiosis take place?
Meiosis takes place in the reproductive organs. In females this is the ovaries, and in males this is the testes.
Define cell differentiation.
Cell differentiation is the process by which cells become specialised to carry out a particular function. During development, cells develop different structures that allow them to perform specific roles.
What is a stem cell?
A stem cell is an undifferentiated cell that can divide and develop into many different types of specialised cells. Stem cells are found in embryos and in some adult tissues.
True or False?
Once a cell has differentiated, it can change into any other type of cell.
False.
Once a cell has become specialised, it cannot change into a different type of cell. Only stem cells retain the ability to divide and differentiate into other cell types.
In an early embryo, cells are called .......... cells and can develop into .......... of the cell types found in the human body.
In an early embryo, cells are called stem cells and can develop into any of the cell types found in the human body.
Why is cell differentiation essential in multicellular organisms?
Cell differentiation is essential in multicellular organisms because it produces a variety of specialised cell types with different functions. Without differentiation, all cells would be identical and unable to carry out the specific roles needed for an organism to survive.
True or False?
Embryonic stem cells can develop into any type of cell in the human body.
True.
Embryonic stem cells are described as pluripotent — they can develop into any cell type. This is why they have potential uses in treating diseases caused by damaged or missing cells.
Define adult stem cells.
Adult stem cells are undifferentiated cells found in specific tissues of the body. They divide to replace damaged cells or produce new cells for growth, but can only form a limited range of cell types.
Complete the table comparing embryonic and adult stem cells.
Feature | Embryonic stem cells | Adult stem cells |
|---|---|---|
Can form any cell type? | No — limited range | |
Main role | Form all tissues during development | |
Location | Early embryo |
Complete the table comparing embryonic and adult stem cells.
Feature | Embryonic stem cells | Adult stem cells |
|---|---|---|
Can form any cell type? | Yes | No — limited range |
Main role | Form all tissues during development | Replace damaged or old cells |
Location | Early embryo | Specific adult tissues |
How do specialised cells differ from stem cells?
Specialised cells differ from stem cells in that they have a fixed structure adapted to carry out a specific function and cannot change into other cell types. Stem cells remain undifferentiated and can still divide to form a range of cell types.
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