Exam code: 8465
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What is a meristem?
A meristem is a region of undifferentiated cells in a plant that actively divide by mitosis to produce new plant tissue. Meristems are found at the growing tips of shoots and roots.

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True or False?
Meristems are found at the growing tips of shoots and roots.
True.
Meristems are located at the growing tips of both shoots and roots, where cells actively divide to produce new plant tissue.
What determines which type of cell a meristem cell becomes?
The type of cell formed depends on the position of the cell in the plant. Meristem cells are undifferentiated and can develop into different plant cell types depending on where they are located.
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What is a meristem?
A meristem is a region of undifferentiated cells in a plant that actively divide by mitosis to produce new plant tissue. Meristems are found at the growing tips of shoots and roots.
True or False?
Meristems are found at the growing tips of shoots and roots.
True.
Meristems are located at the growing tips of both shoots and roots, where cells actively divide to produce new plant tissue.
What determines which type of cell a meristem cell becomes?
The type of cell formed depends on the position of the cell in the plant. Meristem cells are undifferentiated and can develop into different plant cell types depending on where they are located.
Meristem cells are .......... and divide by .......... to produce new plant tissue.
Meristem cells are undifferentiated and divide by mitosis to produce new plant tissue.
True or False?
Cloning rare plants from meristem cells increases both the number of plants and the genetic diversity of that species.
False.
Although cloning increases the number of plants, the plants produced are clones so genetic diversity within that group remains low.
Give two reasons why scientists clone rare plant species at risk of extinction.
To increase plant numbers and maintain biodiversity.
To give scientists more time to study the plants to find out if they produce any useful compounds.
Plant stem cells can be obtained from .......... and used to produce .......... of plants quickly and economically.
Plant stem cells can be obtained from meristems and used to produce clones of plants quickly and economically.
Why might a farmer want to clone a disease-resistant crop plant?
Cloning a disease-resistant crop produces large numbers of identical plants with the same resistance. This reduces the need for pesticides and other chemicals, which has environmental benefits.
What is xylem?
Xylem is a plant tissue that transports water and mineral ions from the roots to the stems and leaves. It is made of hollow tubes of dead cells strengthened by lignin.
True or False?
Xylem transports water from the roots upwards towards the leaves.
True.
Xylem carries water and mineral ions in one direction only — from the roots upwards through the stem to the leaves in the transpiration stream.
How are root hair cells adapted for the efficient uptake of water?
Root hair cells have long extensions that increase the surface area of the root, which increases the rate of water absorption by osmosis from the soil.
Mineral ions are taken up by root hair cells from the soil by .........., while water is absorbed by ...........
Mineral ions are taken up by root hair cells from the soil by active transport, while water is absorbed by osmosis.
True or False?
Nitrate ions are needed by plants to make chlorophyll.
False.
Magnesium ions are needed to make chlorophyll. Nitrate ions are needed to make proteins.
Why do multicellular plants need a transport system?
Diffusion, osmosis and active transport are too slow to meet all of a large organism's needs. A transport system moves substances such as water, mineral ions and sugars between organs efficiently.
During photosynthesis, .......... diffuses into the leaf and .......... diffuses out of the leaf.
During photosynthesis, carbon dioxide diffuses into the leaf and oxygen diffuses out of the leaf.
What gases enter and leave a plant leaf at night, and by what process?
At night, only respiration occurs. Oxygen diffuses into the leaf (it is used in respiration) and carbon dioxide diffuses out of the leaf, both moving down their concentration gradients.
True or False?
Root hairs increase the surface area of roots to improve absorption of water and minerals.
True.
Root hairs are single-celled extensions that greatly increase the surface area of the root, improving absorption of water by osmosis and mineral ions by active transport.
What is transpiration?
Transpiration is the process by which water evaporates from the surfaces of plant leaves and diffuses out through the stomata as water vapour, creating a continuous flow of water up through the plant.
True or False?
Stomata open when guard cells gain water and become turgid.
True.
When water moves into the guard cells by osmosis, they become turgid, pulling apart and opening the stomatal pore to allow gas exchange and water loss.
What is the transpiration stream?
The transpiration stream is the continuous upward movement of water through the xylem, from the roots to the leaves, driven by the evaporation of water from the leaf surface.
The rate of transpiration increases with higher .........., higher .........., and increased air movement.
The rate of transpiration increases with higher temperature, higher light intensity, and increased air movement.
How does high temperature increase the rate of transpiration?
Higher temperature increases the rate at which water evaporates from the surface of mesophyll cells inside the leaf, so water vapour diffuses out through the stomata more quickly.
True or False?
Xylem transports substances in both directions through the plant.
False.
Xylem only transports water and mineral ions in one direction — upwards from roots to leaves. It is phloem that transports sugars in both directions.
How do stomata close, and why?
When guard cells lose water by osmosis to neighbouring cells, they become flaccid, causing the stomatal pore to close. This prevents further water loss when water availability is low.
In the leaves, water evaporates from .......... cells and is lost through .......... as water vapour.
In the leaves, water evaporates from mesophyll cells and is lost through stomata as water vapour.
Why does high light intensity increase the rate of transpiration?
High light intensity causes the stomata to open wider, which increases the rate of water vapour diffusing out of the leaf, so the overall rate of transpiration increases.
What is chromatography?
Chromatography is a technique used to separate and identify the components of a mixture. It uses a mobile phase (solvent) and a stationary phase (e.g. paper) to separate substances based on their different solubilities.
True or False?
In paper chromatography, the baseline should be drawn in pen rather than pencil.
False.
The baseline must be drawn in pencil because ink would dissolve in the solvent and run up the chromatography paper along with the sample, interfering with the results.
In paper chromatography, what are the mobile phase and the stationary phase?
The mobile phase is the liquid solvent that carries the sample up the paper.
The stationary phase is the chromatography paper itself, which does not move.
Rf = distance moved by .......... ÷ distance moved by ..........
Rf = distance moved by substance ÷ distance moved by solvent
What does a pure substance produce on a chromatogram, and why?
A pure substance produces only one spot on the chromatogram, because it contains only a single compound that travels a fixed distance in the given solvent.
True or False?
The Rf value of a substance is always the same regardless of the solvent used.
False.
The Rf value of a substance depends on the solvent used. Changing the solvent changes the Rf value, so the same solvent must be used when comparing Rf values.
Why does a more soluble substance travel further up the chromatography paper?
A more soluble substance spends more time in the mobile phase (solvent) and is carried further up the paper. Less soluble substances spend more time in the stationary phase and travel a shorter distance.
What is an Rf value?
An Rf value (retention factor) is the ratio of the distance moved by a substance to the distance moved by the solvent. It is always less than 1 and has no units.
An impure substance will produce .......... spots on a chromatogram, while a pure substance produces only ...........
An impure substance will produce multiple spots on a chromatogram, while a pure substance produces only one.
What is the purpose of paper chromatography?
To investigate how paper chromatography can be used to separate and identify a mixture of food colourings, and to calculate Rf values for each component.
True or False?
In paper chromatography, the pencil baseline must be placed above the level of the solvent in the beaker.
True.
The baseline must sit above the solvent level. If the baseline is submerged, the samples will wash off into the solvent rather than being carried up the paper.
In paper chromatography, the solvent travels up the paper by .........., carrying the food colouring spots with it.
In paper chromatography, the solvent travels up the paper by capillary action, carrying the food colouring spots with it.
Why must the spots and labels on a chromatogram be made using pencil rather than pen?
Ink from a pen would dissolve in the solvent and run up the chromatography paper, interfering with the results. Pencil does not dissolve, so it stays in place.
True or False?
The Rf value has units of millimetres (mm).
False.
The Rf value is a ratio of two distances, so the units cancel. Rf values are unitless and always have a value less than 1.
How can chromatography be used to identify an unknown food colouring?
Calculate the Rf value of each spot in the unknown sample. Compare these values with the Rf values of known food colourings run under the same conditions. A match in Rf value indicates the same substance.
After the chromatography run, a pencil line is drawn at the .......... to mark how far the .......... has travelled.
After the chromatography run, a pencil line is drawn at the solvent front to mark how far the solvent has travelled.
In paper chromatography, what does it tell you if unknown mixture U produces a spot with the same Rf value as known colouring A?
It suggests that the unknown mixture U contains the same substance as known colouring A, since the same compound has the same Rf value under the same conditions.
What is photosynthesis?
Photosynthesis is an endothermic reaction in which plants use light energy, carbon dioxide and water to produce glucose and oxygen. It takes place in the chloroplasts of plant cells.
True or False?
Photosynthesis is an exothermic reaction.
False.
Photosynthesis is an endothermic reaction — it transfers energy from the environment (in the form of light) to the plant. Energy is stored in the glucose produced.
State the word equation for photosynthesis.
Carbon dioxide + water → glucose + oxygen
Light energy is required for this reaction to occur.
Glucose produced in photosynthesis can be used for .........., converted to insoluble .......... for storage, or used to make .......... which strengthens cell walls.
Glucose produced in photosynthesis can be used for respiration, converted to insoluble starch for storage, or used to make cellulose which strengthens cell walls.
Why are plants called producers in food chains?
Plants are called producers because they make their own food (glucose) using light energy through photosynthesis, rather than consuming other organisms.
True or False?
Plants store excess glucose directly as glucose in their stems, leaves and roots.
False.
Plants convert excess glucose into insoluble starch for storage. Starch is insoluble, which means it does not affect the water potential of cells.
Give two uses of glucose in plants other than respiration and starch storage.
To produce cellulose for strengthening cell walls.
To produce fat or oil for storage (especially in seeds).
Combined with nitrate ions to make amino acids for protein synthesis.
(Any two)
To make amino acids for protein synthesis, plants combine glucose with .......... ions absorbed from the soil.
To make amino acids for protein synthesis, plants combine glucose with nitrate ions absorbed from the soil.
Where does most photosynthesis take place in a plant, and why?
Most photosynthesis takes place in the leaves, in mesophyll cells that are packed with chloroplasts containing chlorophyll to absorb as much light energy as possible.
True or False?
Chloroplasts contain chlorophyll, which absorbs sunlight for photosynthesis.
True.
Chloroplasts are the organelles where photosynthesis occurs. They contain chlorophyll, the pigment that absorbs light energy to drive the photosynthesis reaction.
Define limiting factor.
A limiting factor is any condition that, when in short supply, slows the rate of photosynthesis. The three main limiting factors are light intensity, carbon dioxide concentration, and temperature.
True or False?
Water is considered a limiting factor of photosynthesis because it is needed as a raw material.
False.
Although water is a raw material for photosynthesis, it is not a limiting factor because the amount needed is small compared to how much is transpired, so there is rarely a shortage.
Why does a very high temperature reduce the rate of photosynthesis?
At very high temperatures, the enzymes controlling photosynthesis become denatured — the active site changes shape and is no longer complementary to its substrate, so the reaction can no longer proceed.
As light intensity increases, the rate of photosynthesis .......... until another .......... prevents the rate from rising further.
As light intensity increases, the rate of photosynthesis increases until another limiting factor prevents the rate from rising further.
If a plant is given more carbon dioxide but the temperature remains very low, what happens to the rate of photosynthesis?
(Higher Tier Only)
The rate of photosynthesis does not increase significantly because temperature is now the limiting factor — increasing a non-limiting factor has no effect on the rate.
True or False?
According to the inverse square law, doubling the distance between a lamp and a plant makes the light intensity four times less.
(Higher Tier Only)
True.
The inverse square law states that light intensity is proportional to 1/d², so when the distance doubles, the light intensity decreases by a factor of four (2² = 4).
Define the inverse square law (photosynthesis).
The inverse square law states that light intensity is inversely proportional to the square of the distance from the source: light intensity ∝ 1/d². Doubling the distance reduces light intensity to a quarter of its original value.
In a greenhouse, farmers carefully control levels of heat, light and .......... to maximise the rate of photosynthesis, but must avoid spending money on a factor that is not the ...........
In a greenhouse, farmers carefully control levels of heat, light and carbon dioxide to maximise the rate of photosynthesis, but must avoid spending money on a factor that is not the limiting factor.
Why is it economically wasteful to keep increasing carbon dioxide concentration in a greenhouse beyond a certain point?
(Higher Tier Only)
Once carbon dioxide concentration rises above the level at which it limits photosynthesis, another factor (such as light or temperature) becomes the limiting factor, so extra CO₂ has no effect on yield and the cost is wasted.
What is the aim of the pondweed photosynthesis experiment?
The aim is to investigate the effect of light intensity on the rate of photosynthesis using an aquatic organism such as pondweed.
In the pondweed experiment, the rate of photosynthesis is measured by counting the number of .......... produced by the plant per minute as the distance from the lamp changes.
In the pondweed experiment, the rate of photosynthesis is measured by counting the number of bubbles produced by the plant per minute as the distance from the lamp changes.
True or False?
Moving the lamp further away from the pondweed increases the rate of photosynthesis.
False.
Moving the lamp further away decreases light intensity, which reduces the rate of photosynthesis — fewer bubbles of oxygen are produced per minute.
Why is a glass tank of water placed between the lamp and the pondweed in the light intensity experiment?
The glass tank of water acts as a heat filter, preventing the lamp from heating the water around the plant. This ensures that temperature is controlled as a variable and does not affect the results.
Define the independent variable in the pondweed light experiment.
The independent variable is the factor deliberately changed by the investigator. In the pondweed experiment, it is the distance of the lamp from the pondweed, which alters the light intensity reaching the plant.
True or False?
To improve the reliability of the pondweed experiment, you should repeat each distance at least twice and calculate a mean.
True.
Repeating measurements and calculating a mean reduces the effect of random errors and improves the reliability of the results.
A more accurate way to measure gas production in the pondweed experiment is to use a gas syringe to collect the .......... of .......... produced, rather than counting bubbles.
A more accurate way to measure gas production in the pondweed experiment is to use a gas syringe to collect the volume of oxygen produced, rather than counting bubbles.
In the pondweed experiment, what happens to the number of bubbles per minute as the lamp is moved closer to the plant?
The number of bubbles per minute increases because the light intensity is greater, providing more energy for photosynthesis and so more oxygen is produced.
Define translocation.
Translocation is the transport of dissolved sugars and other soluble products of photosynthesis through the phloem from sources (where they are made) to sinks (where they are used or stored).
True or False?
Phloem tissue is made of dead cells, just like xylem.
False.
Phloem is made of living, elongated cells. It is xylem that is made of dead cells. This difference is important for the function of each tissue.
What is the main sugar transported in the phloem during translocation?
The main sugar transported in the phloem is sucrose, which is dissolved in cell sap and moves from sources (such as leaves) to sinks (such as roots or growing tissues).
In translocation, substances move from a .......... (where they are made) to a .......... (where they are used or stored) through the phloem.
In translocation, substances move from a source (where they are made) to a sink (where they are used or stored) through the phloem.
Define sieve plates.
Sieve plates are perforated end walls found between phloem cells. Their pores allow cell sap containing dissolved sugars and other substances to flow freely between adjacent phloem cells during translocation.
True or False?
During winter, roots act as sinks because sucrose and amino acids are transported from storage organs to other parts of the plant.
True.
In winter, storage organs act as sources and release sucrose and amino acids into the phloem so that other parts of the plant can continue respiration.
How does the structure of phloem cells allow substances to move between them?
Phloem cells are joined end to end, and their end walls contain sieve plates with pores. These pores allow cell sap containing dissolved sugars and nutrients to pass freely from one cell to the next.
After a period of growth, leaves act as the .......... and roots act as .........., storing sucrose as starch.
After a period of growth, leaves act as the source and roots act as sinks, storing sucrose as starch.
Define communicable disease.
A communicable disease is a disease caused by a pathogen that can be transmitted (spread) between individuals within a population. Both plants and animals can be affected.
What type of pathogen causes tobacco mosaic virus (TMV)?
TMV is caused by a virus. It is a widespread plant pathogen affecting approximately 150 species of plants, including tomatoes and cucumbers.
True or False?
Tobacco mosaic virus causes a distinctive mosaic pattern of discolouration on the leaves of infected plants.
True.
Tobacco mosaic virus (TMV) produces a distinctive mosaic pattern of discolouration on the leaves, which reduces the plant's ability to photosynthesise and stunts growth.
Define rose black spot.
Rose black spot is a fungal disease in which purple or black spots develop on the leaves of roses. Affected leaves often turn yellow and drop early, reducing the plant's ability to photosynthesise.
Rose black spot is spread through the environment by .......... or .........., and can be treated using fungicides or by removing affected leaves.
Rose black spot is spread through the environment by water or wind, and can be treated using fungicides or by removing affected leaves.
Why does rose black spot reduce the growth of an affected plant?
Rose black spot causes leaves to develop black spots, turn yellow, and fall early. This reduces the leaf area available for photosynthesis, so the plant produces less glucose for growth.
True or False?
Rose black spot is caused by a virus.
False.
Rose black spot is caused by a fungus, not a virus. It is treated with fungicides or by removing and destroying the affected leaves.
Tobacco mosaic virus affects the growth of a plant because the .......... pattern on the leaves reduces the plant's ability to carry out ...........
Tobacco mosaic virus affects the growth of a plant because the mosaic pattern on the leaves reduces the plant's ability to carry out photosynthesis.
Give two ways that plant diseases caused by pathogens can be spread in the environment.
Plant diseases can be spread by:
Water (e.g. splashing rain carrying fungal spores)
Wind (e.g. carrying spores or viral particles between plants)
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