Required Practical 9: Paper Chromatography (AQA GCSE Combined Science: Synergy: Life & Environmental Sciences): Revision Note

Required practical 9: Using paper chromatography to separate substances

Objective

Investigate how paper chromatography can be used to separate and identify a mixture of food colourings

Hypothesis

R_f values can be used to identify the components of an unknown mixture by comparison with R_f values of known substances

Materials

• A 250 cm³ beaker

• A wooden spill

• A rectangle of chromatography paper

• Four known food colourings labelled A–D

• An unknown mixture of food colourings labelled U

• Five glass capillary tubes

• Paper clip

• Ruler & pencil

Chromatography apparatus

Diagram of the apparatus needed

Practical tip

The pencil line must never be below the level of the solvent as the samples will be washed away

Method

1. Use a ruler to draw a horizontal pencil line 2 cm from the end of the chromatography paper

2. Use a different capillary tube to put a tiny spot of each colouring A, B, C and D on the line

3. Use the fifth tube to put a small spot of the unknown mixture U on the line

4. Make sure each spot is no more than 2-3 mm in diameter and label each spot in pencil

5. Pour water into the beaker to a depth of no more than 1 cm and clip the top of the chromatography paper to the wooden spill. The top end is the furthest from the spots

6. Carefully rest the wooden spill on the top edge of the beaker. The bottom edge of the paper should dip into the solvent

7. Allow the solvent to travel undisturbed at least three quarters of the way up the paper

8. Remove the paper and draw another pencil line on the dry part of the paper as close to the wet edge as possible. This is called the solvent front line

9. Measure the distance in mm between the two pencil lines. This is the distance travelled by the water solvent

10. For each food colour A, B, C and D measure the distance in mm from the start line to the middle of the spot

Results

Record your results in a suitable table

Evaluation

• The R_f values of food colours A, B, C and D should be compared to that for the unknown sample as well as a visual comparison being made

Conclusion

• The use of chromatography and R_f values is a viable method of identifying unknown mixtures given reference material