Transpiration (AQA GCSE Combined Science: Synergy: Life & Environmental Sciences): Revision Note
Exam code: 8465
Transpiration
An overview of transpiration
Transpiration is caused by the evaporation and diffusion of water from the surfaces of the plant
Transpiration primarily occurs within the leaves
As evaporation and diffusion of water creates a net loss of water in the leaf, water is drawn up through the xylem to replace it
Consequently, more water is absorbed by the roots
This creates a constant transpiration stream

Water uptake in the root
Water and mineral ions are taken into the plant via the roots and root hair cells
Root hairs are single-celled extensions of epidermis cells in the root which increase the surface area of the cells significantly; this increases the rate of the absorption of water by osmosis and mineral ions by active transport
They grow between soil particles and are adapted for the efficient uptake of water and mineral ions from the soil
Water and minerals enter the roots as follows:
minerals are taken up from the soil by either active transport or diffusion, depending on soil mineral concentrations
mineral ions lower the water potential of the root hair cells, and water enters the cells from the soil by osmosis

Water movement in the xylem
Water moves into the xylem by osmosis and is drawn upwards, towards the leaves, due to transpiration
Movement in the xylem only takes place in one direction - from roots to leaves
The role of the xylem is to transport water and mineral ions from the roots to other parts of the plant
The upward movement of water in the xylem is known as the transpiration stream
Structure of the xylem
The xylem is formed from a hollow tube of dead cells, reinforced by lignin, which provides a route for the column of water to move through the plant by transpiration

Water loss in the leaves
In the leaves, water moves from the xylem into spongy mesophyll
Water evaporates from the surface of the mesophyll cells and is lost through the stomata as water vapour

Stomata
Stomata are spaces found between two guard cells predominantly on the lower epidermis of the leaf
The guard cells open and close the stomatal pore, controlling gas exchange and water loss
Stomata open when water moves (by osmosis) into the guard cells causing them to become turgid
This allows gases to diffuse in and out of the leaf through the stomatal pore
Stomata tend to open when there is plenty of water and sunlight
Stomata close when the guard cells lose water (by osmosis) to the neighbouring epidermal cells and they become flaccid
This prevents any diffusion into or out of the leaf
Stomata tend to close due to low water availability or low sunlight


Factors Affecting Transpiration
Air movement, humidity, temperature and light intensity all have an effect on the rate at which transpiration occurs
The table below explains how these four factors affect the rate of transpiration when they are all high; the opposite effect would be observed if they were low

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