Transpiration (AQA GCSE Combined Science: Synergy: Life & Environmental Sciences): Revision Note

Exam code: 8465

Ruth Brindle

Written by: Ruth Brindle

Reviewed by: Lára Marie McIvor

Updated on

Transpiration

An overview of transpiration

  • Transpiration is caused by the evaporation and diffusion of water from the surfaces of the plant

    • Transpiration primarily occurs within the leaves

  •  As evaporation and diffusion of water creates a net loss of water in the leaf, water is drawn up through the xylem to replace it

    • Consequently, more water is absorbed by the roots

    • This creates a constant transpiration stream

Diagram illustrating xylem structure and water movement in plants, from roots through xylem to leaves, highlighting key features like lignin and cell walls.
The route of water movement through a plant

Water uptake in the root

  • Water and mineral ions are taken into the plant via the roots and root hair cells

  • Root hairs are single-celled extensions of epidermis cells in the root which increase the surface area of the cells significantly; this increases the rate of the absorption of water by osmosis and mineral ions by active transport

  • They grow between soil particles and are adapted for the efficient uptake of water and mineral ions from the soil

  • Water and minerals enter the roots as follows:

    • minerals are taken up from the soil by either active transport or diffusion, depending on soil mineral concentrations

    • mineral ions lower the water potential of the root hair cells, and water enters the cells from the soil by osmosis

Diagram illustrating osmosis in plant roots, showing water moving from soil to root hair cells, then through root cortex cells to xylem vessels.
The structure of a root specifically allows it to maximise absorption of water by osmosis and mineral ions by active transport

Water movement in the xylem

  • Water moves into the xylem by osmosis and is drawn upwards, towards the leaves, due to transpiration

  •  Movement in the xylem only takes place in one direction - from roots to leaves 

  • The role of the xylem is to transport water and mineral ions from the roots to other parts of the plant

  • The upward movement of water in the xylem is known as the transpiration stream

Structure of the xylem

  • The xylem is formed from a hollow tube of dead cells, reinforced by lignin, which provides a route for the column of water to move through the plant by transpiration

Diagram of a xylem vessel showing cell walls thickened with lignin, no cell contents, water column, and broken-down walls between cells.
The structure of the xylem allows it to function as a vessel for the transport of water through the plant

Water loss in the leaves

  • In the leaves, water moves from the xylem into spongy mesophyll

  • Water evaporates from the surface of the mesophyll cells and is lost through the stomata as water vapour

Diagram showing water movement in a plant: evaporation from mesophyll cells to air space and diffusion out of open stoma through guard cells.
Water moves from the xylem to spongy mesophyll cells and is lost through the stomata as water vapour

Stomata

  • Stomata are spaces found between two guard cells predominantly on the lower epidermis of the leaf

  • The guard cells open and close the stomatal pore, controlling gas exchange and water loss

  • Stomata open when water moves (by osmosis) into the guard cells causing them to become turgid

    • This allows gases to diffuse in and out of the leaf through the stomatal pore

    • Stomata tend to open when there is plenty of water and sunlight

  • Stomata close when the guard cells lose water (by osmosis) to the neighbouring epidermal cells and they become flaccid

    • This prevents any diffusion into or out of the leaf

    • Stomata tend to close due to low water availability or low sunlight

Guard cells and stomata 1, downloadable IGCSE & GCSE Biology revision notes
Diagram showing plant stomatal pore with turgid guard cells when open and flaccid when closed. Labels: chloroplast, vacuole, cell wall, nucleus.
The guard cells control the opening and closing of the stomata

Factors Affecting Transpiration

  • Air movement, humidity, temperature and light intensity all have an effect on the rate at which transpiration occurs

  • The table below explains how these four factors affect the rate of transpiration when they are all high; the opposite effect would be observed if they were low

Transpiration factors table, downloadable IGCSE & GCSE Biology revision notes

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Ruth Brindle

Author: Ruth Brindle

Expertise: Biology Content Creator

Ruth graduated from Sheffield University with a degree in Biology and went on to teach Science in London whilst also completing an MA in innovation in Education. With 10 years of teaching experience across the 3 key science disciplines, Ruth decided to set up a tutoring business to support students in her local area. Ruth has worked with several exam boards and loves to use her experience to produce educational materials which make the mark schemes accessible to all students.

Lára Marie McIvor

Reviewer: Lára Marie McIvor

Expertise: Biology, Psychology & Sociology Subject Lead

Lára graduated from Oxford University in Biological Sciences and has now been a science tutor working in the UK for several years. Lára has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning.