Factors Affecting The Rate Of Photosynthesis (AQA GCSE Combined Science: Synergy: Life & Environmental Sciences): Revision Note

Exam code: 8465

Ruth Brindle

Written by: Ruth Brindle

Reviewed by: Lára Marie McIvor

Updated on

Limiting factors

  • In order to photosynthesise a plant needs light, water and carbon dioxide

  • The availability of light and carbon dioxide can affect how much and how quickly (the rate) photosynthesis occurs

    • Although water is necessary for photosynthesis, it is not considered a limiting factor as the amount needed is relatively small compared to the amount of water transpired from a plant so there is hardly ever a situation where there is not enough water for photosynthesis

  • Other environmental factors such as temperature and the amount of chlorophyll in the chloroplasts can also affect the rate of photosynthesis

Temperature

  • The temperature of the environment affects how much kinetic energy all particles have – so temperature affects the speed at which carbon dioxide and water move through a plant

  • The lower the temperature, the less kinetic energy particles have, resulting in fewer successful collisions occurring over a period of time

  • Increasing temperature increases the kinetic energy of particles, increasing the likelihood of collisions between reactants and enzymes which results in the formation of products

  • At higher temperatures, however, enzymes that control the processes of photosynthesis can be denatured (where the active site changes shape and is no longer complementary to its substrate) – this reduces the overall rate

The effect of temperature on the rate of photosynthesis, IGCSE & GCSE Biology revision notes

Graph showing the effect of temperature on the rate of photosynthesis

Light

  • The intensity of the light available to the plant will depend on the amount of energy that it has to carry out photosynthesis

  • The more light a plant receives, the faster the rate of photosynthesis

  • This trend will continue until some other factor required for photosynthesis prevents the rate from increasing further because it is now in short supply

The effect of light intensity on the rate of photosynthesis, IGCSE & GCSE Biology revision notes

Graph showing the effect of light intensity on the rate of photosynthesis

At low light intensities, increasing the intensity will initially increase the rate of photosynthesis. At a certain point, increasing the light intensity stops increasing the rate. The rate becomes constant regardless of how much light intensity increases as something else is limiting the rate.

Carbon dioxide concentration

  • Carbon dioxide is one of the raw materials required for photosynthesis

  • This means the more carbon dioxide that is present, the faster the reaction can occur

  • This trend will continue until some other factor required for photosynthesis prevents the rate from increasing further because it is now in short supply

The effect of carbon dioxide concentration on the rate of photosynthesis, IGCSE & GCSE Biology revision notes

A graph showing the effect of the concentration of carbon dioxide on the rate of photosynthesis

Examiner Tips and Tricks

Make sure you know that the effect of low temperature is a reduced rate of collisions occurring, and the effect of high temperatures is that enzymes denature.

Interactions of Limiting Factors (HT Only)

Higher tier only

  • More than one limiting factor can have an effect on the rate of photosynthesis

  • Graphs may show the effect of two factors interacting:

Light intensity and temperature, downloadable IGCSE & GCSE Biology revision notes

 The rate of photosynthesis increases with increasing light intensity, temperature and carbon dioxide

 At the start of the graph, the rate of photosynthesis is limited by the light intensity so both lines are showing the same rate.

As the light intensity increases the rate of photosynthesis at 15℃ is lower than 25℃.

Both lines level off, this shows that light intensity is no longer the limiting factor.

 

  • Graphs may show the interactions between three different factors, the graph below shows the relationship between temperature, carbon dioxide as light intensity is increased:

Light Intensity, CO2 and Temperature, downloadable IGCSE & GCSE Biology revision notes

 

The rate of photosynthesis increases with increasing light intensity, temperature and carbon dioxide

 All three experiments level off when light intensity is no longer the limiting factor.

In the top line, this has the highest temperature and concentration of carbon dioxide so the rate of photosynthesis is much higher.

In experiment 2, the concentration of carbon dioxide is the limiting factor.

In this graph, the rate of photosynthesis is controlled by carbon dioxide levels.

Light Intensity (HT Only)

Higher tier only

  • The inverse square law shows the relationship between light intensity and distance.

  • As the distance increases the light intensity decreases. This means that they are inversely proportional to each other

  • Light intensity and distance are inversely proportional to each other

  • This means that as the distance doubles you decrease the intensity of the light will be four times less

  • This is called the inverse square law and shown by the equation below:

Inverse Square Law Equation, downloadable IGCSE & GCSE Biology revision notes

Worked Example

Calculate the light intensity when the distance of the plant is 30cm from the lamp

Answer:

  1. Use the equation:

    Light intensity =  1/d2

  2. Fill in the values you know:

    Light intensity = 1/302

  3. Calculate the light intensity:

    Light intensity = 0.001 au

Examiner Tips and Tricks

Remember that ‘au’ stands for arbitrary units.

Growing in a Greenhouse (HT Only)

Higher tier only

  • Commercial horticulturists will grow their plants in a greenhouse

  • This means that they are able to control as many of the limiting factors of photosynthesis as possible

  • Limiting factors are important in the economics of enhancing the conditions in greenhouses to gain the maximum rate of photosynthesis while still maintaining profit

    • Keeping plants in a greenhouse has associated costs, but the increased yield of the crop and fact that the crop can be harvested more frequently, means that the farmer will make more money

  • The levels of heat, light, water, carbon dioxide and nutrients are carefully controlled so only the smallest amounts needed are used so that farmers are not wasting money

    • Eg. spending money on increasing the concentration of carbon dioxide beyond a point when some other factor limits the rate of photosynthesis is a waste

Commercial Growing in a Greenhouse, downloadable IGCSE & GCSE Biology revision notes

The farmers can increase yield in a greenhouse but this does have an increased cost

Examiner Tips and Tricks

You should be able to use data in the exam to relate limiting factors to the cost-effectiveness of adding heat, light or carbon dioxide to greenhouses. Remember that spending money on increasing a factor above the point at which it has an effect on the rate of photosynthesis will be a waste of money.

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Ruth Brindle

Author: Ruth Brindle

Expertise: Biology Content Creator

Ruth graduated from Sheffield University with a degree in Biology and went on to teach Science in London whilst also completing an MA in innovation in Education. With 10 years of teaching experience across the 3 key science disciplines, Ruth decided to set up a tutoring business to support students in her local area. Ruth has worked with several exam boards and loves to use her experience to produce educational materials which make the mark schemes accessible to all students.

Lára Marie McIvor

Reviewer: Lára Marie McIvor

Expertise: Biology, Psychology & Sociology Subject Lead

Lára graduated from Oxford University in Biological Sciences and has now been a science tutor working in the UK for several years. Lára has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning.