Factors Affecting The Rate Of Photosynthesis (AQA GCSE Combined Science: Synergy: Life & Environmental Sciences): Revision Note
Exam code: 8465
Limiting factors
In order to photosynthesise a plant needs light, water and carbon dioxide
The availability of light and carbon dioxide can affect how much and how quickly (the rate) photosynthesis occurs
Although water is necessary for photosynthesis, it is not considered a limiting factor as the amount needed is relatively small compared to the amount of water transpired from a plant so there is hardly ever a situation where there is not enough water for photosynthesis
Other environmental factors such as temperature and the amount of chlorophyll in the chloroplasts can also affect the rate of photosynthesis
Temperature
The temperature of the environment affects how much kinetic energy all particles have – so temperature affects the speed at which carbon dioxide and water move through a plant
The lower the temperature, the less kinetic energy particles have, resulting in fewer successful collisions occurring over a period of time
Increasing temperature increases the kinetic energy of particles, increasing the likelihood of collisions between reactants and enzymes which results in the formation of products
At higher temperatures, however, enzymes that control the processes of photosynthesis can be denatured (where the active site changes shape and is no longer complementary to its substrate) – this reduces the overall rate

Graph showing the effect of temperature on the rate of photosynthesis
Light
The intensity of the light available to the plant will depend on the amount of energy that it has to carry out photosynthesis
The more light a plant receives, the faster the rate of photosynthesis
This trend will continue until some other factor required for photosynthesis prevents the rate from increasing further because it is now in short supply

Graph showing the effect of light intensity on the rate of photosynthesis
At low light intensities, increasing the intensity will initially increase the rate of photosynthesis. At a certain point, increasing the light intensity stops increasing the rate. The rate becomes constant regardless of how much light intensity increases as something else is limiting the rate.
Carbon dioxide concentration
Carbon dioxide is one of the raw materials required for photosynthesis
This means the more carbon dioxide that is present, the faster the reaction can occur
This trend will continue until some other factor required for photosynthesis prevents the rate from increasing further because it is now in short supply

A graph showing the effect of the concentration of carbon dioxide on the rate of photosynthesis
Examiner Tips and Tricks
Make sure you know that the effect of low temperature is a reduced rate of collisions occurring, and the effect of high temperatures is that enzymes denature.
Interactions of Limiting Factors (HT Only)
Higher tier only
More than one limiting factor can have an effect on the rate of photosynthesis
Graphs may show the effect of two factors interacting:

The rate of photosynthesis increases with increasing light intensity, temperature and carbon dioxide
At the start of the graph, the rate of photosynthesis is limited by the light intensity so both lines are showing the same rate.
As the light intensity increases the rate of photosynthesis at 15℃ is lower than 25℃.
Both lines level off, this shows that light intensity is no longer the limiting factor.
Graphs may show the interactions between three different factors, the graph below shows the relationship between temperature, carbon dioxide as light intensity is increased:

The rate of photosynthesis increases with increasing light intensity, temperature and carbon dioxide
All three experiments level off when light intensity is no longer the limiting factor.
In the top line, this has the highest temperature and concentration of carbon dioxide so the rate of photosynthesis is much higher.
In experiment 2, the concentration of carbon dioxide is the limiting factor.
In this graph, the rate of photosynthesis is controlled by carbon dioxide levels.
Light Intensity (HT Only)
Higher tier only
The inverse square law shows the relationship between light intensity and distance.
As the distance increases the light intensity decreases. This means that they are inversely proportional to each other
Light intensity and distance are inversely proportional to each other
This means that as the distance doubles you decrease the intensity of the light will be four times less
This is called the inverse square law and shown by the equation below:

Worked Example
Calculate the light intensity when the distance of the plant is 30cm from the lamp
Answer:
Use the equation:
Light intensity = 1/d2
Fill in the values you know:
Light intensity = 1/302
Calculate the light intensity:
Light intensity = 0.001 au
Examiner Tips and Tricks
Remember that ‘au’ stands for arbitrary units.
Growing in a Greenhouse (HT Only)
Higher tier only
Commercial horticulturists will grow their plants in a greenhouse
This means that they are able to control as many of the limiting factors of photosynthesis as possible
Limiting factors are important in the economics of enhancing the conditions in greenhouses to gain the maximum rate of photosynthesis while still maintaining profit
Keeping plants in a greenhouse has associated costs, but the increased yield of the crop and fact that the crop can be harvested more frequently, means that the farmer will make more money
The levels of heat, light, water, carbon dioxide and nutrients are carefully controlled so only the smallest amounts needed are used so that farmers are not wasting money
Eg. spending money on increasing the concentration of carbon dioxide beyond a point when some other factor limits the rate of photosynthesis is a waste

The farmers can increase yield in a greenhouse but this does have an increased cost
Examiner Tips and Tricks
You should be able to use data in the exam to relate limiting factors to the cost-effectiveness of adding heat, light or carbon dioxide to greenhouses. Remember that spending money on increasing a factor above the point at which it has an effect on the rate of photosynthesis will be a waste of money.
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