Exam code: 3430
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Define digestion.
The process in which large, insoluble molecules in food are broken down into smaller, soluble molecules that can be absorbed into the bloodstream.

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Why do large food molecules need to be digested?
They are too large and insoluble to be absorbed into the bloodstream. They must be broken down into small, soluble molecules first.
What are the small soluble products of digestion used for?
To provide cells with energy, through respiration
To provide materials for cells to grow, repair and function
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Define digestion.
The process in which large, insoluble molecules in food are broken down into smaller, soluble molecules that can be absorbed into the bloodstream.
Why do large food molecules need to be digested?
They are too large and insoluble to be absorbed into the bloodstream. They must be broken down into small, soluble molecules first.
What are the small soluble products of digestion used for?
To provide cells with energy, through respiration
To provide materials for cells to grow, repair and function
What are fats made up of?
Fatty acids and glycerol.
What are proteins made up of?
Amino acids.
What is starch made up of?
A chain of glucose molecules. Starch is a carbohydrate.
Fats are digested into fatty acids and ________.
Fats are digested into fatty acids and glycerol.
Proteins are digested into ________.
Proteins are digested into amino acids.
Starch is digested into simple sugars such as ________.
Starch is digested into simple sugars such as glucose.
Give an example of a large insoluble molecule and the soluble molecule it becomes.
Starch, a large insoluble carbohydrate, is digested into glucose, a small soluble sugar.
True or False?
The digestive system is an example of an organ system.
True.
Several organs work together in the digestive system to digest and absorb food.
True or False?
Glucose molecules must be digested before they can be absorbed.
False.
Glucose is already small and soluble, so it can be absorbed directly without further digestion.
Where do the products of digestion need to get to, and how?
They are absorbed into the bloodstream and delivered to the cells of the body.
Why is starch insoluble but glucose soluble?
Starch is a long chain of many glucose molecules, making it too large to dissolve or be absorbed. Glucose is a single small molecule.
Describe how to prepare a solid food sample for food testing.
Break up the food using a pestle and mortar.
Transfer it to a test tube and add distilled water, stirring with a glass rod.
Filter the mixture using a funnel and filter paper, and collect the solution.
Which reagent is used to test for starch, and what is a positive result?
Iodine solution.
A positive result is a colour change from orange/brown to blue/black.
Describe the test for glucose.
Add Benedict's reagent to the food solution in a test tube.
Heat in a boiling water bath for about 5 minutes.
A positive result is a colour change from blue to brick red.
Which reagent is used to test for protein, and what is a positive result?
Biuret solution.
A positive result is a colour change from blue to violet.
Which food test requires heating, and why?
The Benedict's test for glucose. The sample must be heated in a boiling water bath for the colour change to occur.
________ solution is used to test for the presence of starch.
Iodine solution is used to test for the presence of starch.
A positive Benedict's test changes colour from blue to ________.
A positive Benedict's test changes colour from blue to brick red.
A positive biuret test for protein changes colour from blue to ________.
A positive biuret test for protein changes colour from blue to violet.
What is a negative result for the iodine test for starch?
The iodine solution stays brown, showing no starch is present.
What is a negative result for the Benedict's test and the biuret test?
Both reagents stay blue, showing that glucose or protein is not present.
True or False?
Describing a positive iodine test as "black" would gain the mark.
False.
The positive result must be described as blue/black, not blue or black alone.
True or False?
A positive Benedict's test should be described as "brick red", not just "red".
True.
The examiner expects the term brick red.
A food sample turns iodine blue/black and biuret violet. What does it contain?
Both starch and protein.
Why must a solid food be ground up and mixed with water before testing?
The reagents need to reach the food molecules in solution. Grinding and dissolving the food makes the test reliable.
State the starting colour of each of the three food test reagents.
Iodine solution: brown
Benedict's reagent: blue
Biuret solution: blue
Name the three main types of digestive enzyme.
Carbohydrases
Proteases
Lipases
Define carbohydrase.
An enzyme that breaks down carbohydrates, such as starch, into simple sugars such as glucose.
Define protease.
An enzyme that breaks down proteins into amino acids.
Define lipase.
An enzyme that breaks down fats and oils into fatty acids and glycerol.
Where are proteases made and where does protein digestion take place?
Proteases are made in the stomach, pancreas and small intestine.
Protein digestion takes place in the stomach and small intestine.
Where is lipase produced and where does it act?
Lipase is produced in the pancreas and secreted into the small intestine, where it acts.
________ break down starch into simple sugars such as glucose.
Carbohydrases break down starch into simple sugars such as glucose.
Lipase breaks down fats into fatty acids and ________.
Lipase breaks down fats into fatty acids and glycerol.
Why are digestive enzymes needed to break down large food molecules?
Large molecules such as starch and protein are held together by chemical bonds. Enzymes speed up the breaking of these bonds so digestion happens fast enough.
Which enzyme would you need to digest a meal high in protein?
Protease, which breaks proteins down into amino acids.
True or False?
A single digestive enzyme can break down starch, protein and fat.
False.
Enzymes are specific. Each type of enzyme acts on only one type of food molecule.
True or False?
Digestive enzymes are used up as they break down food.
False.
Enzymes are catalysts, so they are not used up or changed by the reaction.
Match each enzyme to the products it makes.
Carbohydrase → simple sugars, e.g. glucose
Protease → amino acids
Lipase → fatty acids and glycerol
Name the ten structures labelled on a digestive system diagram.
Mouth
Oesophagus
Stomach
Liver
Gall bladder
Bile duct
Pancreas
Small intestine
Large intestine
Anus
Describe what happens to food in the mouth.
Food is physically broken down into smaller pieces, increasing its surface area.
Carbohydrase begins the breakdown of starch.
The food is moistened and shaped ready for swallowing.
Describe the role of the stomach in digestion.
Food is churned and mixed, which physically breaks it up.
Protease enzymes begin the chemical digestion of proteins into amino acids.
What is the role of the pancreas in digestion?
It produces all three types of digestive enzyme — carbohydrases, proteases and lipases — and secretes them into the small intestine.
What are the two roles of the small intestine?
Digestion is completed by enzymes made there and secreted from the pancreas
Absorption of the digested food molecules into the bloodstream
What is the role of the large intestine?
Water is absorbed from the remaining waste material.
The solid waste that is left is removed through the anus.
What is the role of the liver in digestion?
It produces and secretes bile, which emulsifies fats.
The ________ produces all three types of digestive enzyme and secretes them into the small intestine.
The pancreas produces all three types of digestive enzyme and secretes them into the small intestine.
Water is absorbed from the remaining waste material in the ________ intestine.
Water is absorbed from the remaining waste material in the large intestine.
True or False?
Chewing food in the mouth breaks the chemical bonds in it.
False.
Chewing breaks food into smaller pieces, increasing its surface area for enzymes to act on. It does not break any chemical bonds.
True or False?
Food passes directly through the liver and pancreas.
False.
The liver and pancreas release substances into the gut, but food does not pass through them.
Why is increasing the surface area of food by chewing useful?
It gives enzymes a larger area to act on, so chemical digestion happens faster.
Define peristalsis.
Waves of muscle contraction in the walls of the digestive system that move food along.
Why is dietary fibre important for peristalsis?
Fibre gives the muscles bulk to push against as they contract, so food keeps moving along.
________ is the wave-like muscle contraction that moves food along the digestive system.
Peristalsis is the wave-like muscle contraction that moves food along the digestive system.
Waves of muscle ________ squeeze food along the digestive system.
Waves of muscle contraction squeeze food along the digestive system.
True or False?
Food is moved through the body by gravity alone.
False.
Food is moved by peristalsis, waves of muscular contraction. This is why you can still swallow while lying down.
True or False?
Peristalsis takes place in the oesophagus and the intestines.
True.
Waves of muscular contraction move food along the whole digestive system.
Explain why a diet low in fibre can make peristalsis less effective.
Without enough fibre, the muscles have less bulk to push against, so food moves along the digestive system more slowly.
Describe how peristalsis moves food along the digestive system.
Muscles in the wall contract rhythmically one section after another.
This creates a wave-like action that squeezes the food mass forwards.
Where is bile produced and where is it stored?
Bile is produced in the liver and stored in the gall bladder.
How does bile travel from the gall bladder to the small intestine?
Through a small tube called the bile duct.
What is the role of bile in the breakdown of fats?
To emulsify fats, breaking large fat droplets into smaller droplets.
This increases their surface area, so lipase can break them down faster.
Define emulsification.
The breaking apart of large fat droplets into smaller ones, which increases their surface area.
Explain how emulsification speeds up the digestion of fats.
Breaking fat into smaller droplets gives a larger surface area.
This gives lipase enzymes more area to act on, so fats are broken down into fatty acids and glycerol faster.
Bile ________ fats, breaking large droplets into smaller ones.
Bile emulsifies fats, breaking large droplets into smaller ones.
Bile is produced in the liver and stored in the ________.
Bile is produced in the liver and stored in the gall bladder.
True or False?
Bile is an enzyme.
False.
Bile is not an enzyme. It emulsifies fats physically, but it does not break any chemical bonds.
Which enzyme works on the fat droplets after bile has emulsified them?
Lipase, which breaks fats down into fatty acids and glycerol.
Predict the effect on fat digestion if a person had their gall bladder removed.
Bile could not be stored and released in large amounts when needed.
Fats would be emulsified less effectively, so lipase would work more slowly and fat digestion would be less efficient.
Why does emulsification not change the chemical structure of fat molecules?
It only breaks large droplets into smaller droplets. No chemical bonds within the fat molecules are broken.
Name the three structures that get bile to the small intestine.
Liver — produces bile
Gall bladder — stores bile
Bile duct — carries bile to the small intestine
Which soluble substances are absorbed in the small intestine?
Glucose
Amino acids
Fatty acids and glycerol
Give three adaptations of the small intestine for rapid absorption.
A large surface area, from its folded, finger-like lining
A short diffusion distance, as the wall is one cell thick
A good blood supply, maintaining a steep concentration gradient
How does a good blood supply help absorption in the small intestine?
It carries glucose and amino acids away from the small intestine, which maintains a steep concentration gradient for diffusion.
The wall of the small intestine is only ________ cell thick, giving a short diffusion distance.
The wall of the small intestine is only one cell thick, giving a short diffusion distance.
Describe how Visking tubing can be used as a model gut.
Fill a section of Visking tubing with a mixture of starch and carbohydrase solutions.
Suspend the tubing in a beaker of water.
Test the water outside the tubing at intervals for starch using iodine and for glucose using Benedict's reagent.
Explain the results of the Visking tubing model gut experiment.
The carbohydrase digests starch into glucose inside the tubing.
Glucose is small enough to diffuse out through the pores, so its concentration in the surrounding water rises.
Starch stays inside, because its molecules are too large to pass through.
How is Visking tubing similar to, and different from, the gut wall?
Similarity: both are partially permeable
Difference: the small intestine has a much larger surface area, and a blood supply that carries absorbed food away
Give one limitation of using distilled water to represent blood in the model gut.
Distilled water does not flow, so it cannot maintain the concentration gradient the way circulating blood does.
Soluble food substances are absorbed into the blood by the process of ________.
Soluble food substances are absorbed into the blood by the process of diffusion.
True or False?
Starch can diffuse through the wall of the small intestine.
False.
Starch molecules are too large. Starch must first be digested into glucose, which is small enough to be absorbed.
Explain why a large surface area speeds up absorption.
More of the food solution is in contact with the absorbing surface at one time, so more molecules can diffuse across per second.
Why is the small intestine very long?
A greater length gives a larger surface area and more time for digested food to be absorbed as it passes through.
What happens to the glucose produced by digesting carbohydrates?
It is used in respiration to provide energy, or it is converted to glycogen and stored.
What happens to the fatty acids and glycerol produced by digesting fats?
They provide energy.
What happens to the amino acids produced by digesting proteins?
They are needed to build proteins in the body.
What is the main function of carbohydrates in the diet?
To act as a source of energy. Glucose from carbohydrate digestion is a reactant for respiration.
What is the main function of protein in the diet?
Growth and repair. Amino acids are used to build new proteins in the body.
What are the main functions of fats in the diet?
Energy storage, in a more condensed form than carbohydrate
Insulation
Excess glucose is converted into ________ and stored in the liver and muscles.
Excess glucose is converted into glycogen and stored in the liver and muscles.
________ from digested proteins are needed to build new proteins in the body.
Amino acids from digested proteins are needed to build new proteins in the body.
True or False?
Glycogen is the storage form of glucose in humans.
True.
Excess glucose is converted to glycogen and stored in the liver and muscle cells.
True or False?
Amino acids are the body's main source of energy.
False.
Glucose from carbohydrates is the main energy source. Amino acids are mainly used for growth and repair.
Why does a growing child need a higher proportion of protein in their diet?
Protein provides the amino acids needed to build new body proteins for growth.
Define balanced diet.
A diet containing all of the food groups in the correct proportions to meet the body's needs.
Name the seven components of a balanced diet.
Protein
Carbohydrates
Fats
Minerals, e.g. iron
Vitamins, e.g. vitamin C
Fibre
Water
Why is iron needed in the diet, and where is it found?
Iron is used to make haemoglobin, which carries oxygen in red blood cells.
Sources include red meat, liver and leafy green vegetables.
Why is vitamin C needed in the diet, and where is it found?
Vitamin C forms an essential part of collagen, the protein that makes up skin, hair, gums and bones.
Sources include citrus fruit, strawberries and green vegetables.
Why is fibre needed in the diet?
Fibre provides bulk (roughage) for the intestine muscles to push against during peristalsis.
Why is water needed in the diet?
Water is needed for the chemical reactions that take place in cells.
Why do different foods have different energy contents?
They contain different quantities of carbohydrate and fat. Fats in particular contain a high density of energy-storing chemical bonds.
What happens to energy from food when it is taken in excess?
It is stored as fat by the body, under the skin and around the organs. Excess carbohydrate is also stored as glycogen.
When energy from food is taken in excess, it is stored as ________ by the body.
When energy from food is taken in excess, it is stored as fat by the body.
Why do athletes often eat pasta or potatoes before competing?
These foods contain starch, which breaks down slowly and releases glucose into the blood over a longer time span.
State the health effects of excess sugar in the diet.
Type 2 diabetes
Obesity
Tooth decay
State the health effects of excess fat in the diet.
Obesity
Heart disease
Circulatory disease
Excess salt in the diet is linked to ________ blood pressure.
Excess salt in the diet is linked to high blood pressure.
Define malnutrition.
The health problems that result from an unbalanced diet, whether from too much or too little of particular nutrients.
True or False?
Malnutrition only means not eating enough food.
False.
Malnutrition results from any unbalanced diet, including eating too much sugar, fat or salt.
Describe the method for investigating the energy content of a food.
Measure 25 cm³ of water into a boiling tube and record its starting temperature.
Weigh the initial mass of the food sample.
Set fire to the food with a Bunsen burner and hold it about 2 cm below the boiling tube until it has completely burned.
Record the final temperature of the water, then repeat with different foods.
How is energy transferred from the food to the water in this experiment?
Energy stored in the food is transferred into the heat of the flames, which in turn heats the water in the boiling tube.
What does a larger rise in water temperature tell you?
The food sample contained more stored energy.
A larger rise in water temperature shows the food contained more ________.
A larger rise in water temperature shows the food contained more energy.
Why must the mass of the food sample be measured before burning?
So the energy released can be compared fairly between foods.
A larger sample would release more energy whatever type of food it was.
Give two limitations of this experiment.
The food may burn incompletely, so not all its energy is released
Heat is lost to the surroundings rather than heating the water
How can the problem of incomplete burning be reduced?
Relight the food sample each time it goes out, until it no longer catches light.
Heat is always lost to the surroundings. Why are the results still useful?
As long as the procedure is carried out in exactly the same way each time, especially the distance from the boiling tube, the results can still be used to compare foods.
Why should nuts be avoided as a food sample in this practical?
Burning them can release allergens into the air, risking an allergic reaction or anaphylactic shock.
Give two control measures for the burning hazards in this practical.
Use tongs or a mounted needle to hold the food, keeping hands away from the flame
Wear safety goggles and point the boiling tube away from you
True or False?
This experiment gives a completely accurate measure of the energy in a food.
False.
Heat is lost to the surroundings and burning may be incomplete, so the calculated value is an underestimate. It is still valid for comparison.
True or False?
A hazard and a risk mean the same thing.
False.
A hazard is something that could cause harm. A risk is the harm that could actually occur. A control measure reduces that chance.
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