Plants & Photosynthesis (WJEC GCSE Science (Double Award): Biology): Exam Questions

Exam code: 3430

1 hour10 questions
1a
1 mark

State the function of chlorophyll.

1b
7 marks

James investigated photosynthesis in a potted plant using the following method:

  • Place a potted plant in the dark for 24 hours to destarch the leaves.

  • After 24 hours, set up the apparatus as shown in in Image 1.1.

Image 1.1

Diagram of a potted plant sealed in a glass bell jar with labelled leaves, sodium hydroxide to absorb carbon dioxide, and a strip light above
  • Leave the apparatus containing the potted plant in bright light for 24 hours.

  • Remove leaves A, B and C, then:

    • remove the card from leaf B

    • place the leaves in boiling water

    • place the leaves in boiling ethanol to remove the chlorophyll

    • dip the leaves in hot water and blot dry

  • Carry out the starch test by dropping iodine solution onto the leaves.

The appearance of each leaf after the starch test is shown in Image 1.2.

Image 1.2

Diagram of three labelled leaves: A fully brown, B partly brown and partly blue-black, and C fully blue-black, illustrating progressive colour change.

(i)

I. Explain the results for leaf A in Image 1.2.

[3]

II. Explain the appearance of the brown band across the middle of leaf B in Image 1.2.

[2]

(ii) Identify the control leaf (A, B, or C) and give the reason for your choice.

Leaf: ..........................................

[1]

Reason: ..........................................

[1]

2a
2 marks

Write the word equation for photosynthesis.

2b
2 marks

The diagram shows leaves from two varieties of geranium A and B of the genus Pelargonium. Light energy falling on the two leaves and the light energy reflected from each leaf, during a period of one hour is also shown.

[Figure: A diagram showing light energy falling on and reflected from leaves of two Pelargonium varieties. Variety A: 25 J light energy falling on leaf, 16 J light energy reflected from leaf, giving 64% light energy reflected (shown). Variety B: 25 J light energy falling on leaf, 20 J light energy reflected from leaf]

Calculate the percentage of light energy falling on leaf B that is reflected.

Write your answer in the diagram.

2c
2 marks

The graph shows the rate of photosynthesis in the two plants between 06.00 hours and 18.00 hours.

[Figure: A graph showing the rate of photosynthesis (arbitrary units) for Variety A and Variety B plotted against time (06.00 to 18.00 hours)]

Use the diagram opposite and the graph above to explain why the mass of variety A is likely to increase at a faster rate than the mass of variety B.

3
1 mark

State one resource oak trees need for growth.

..........

4a
6 marks

Describe how you would investigate the effect of light intensity on the rate of photosynthesis using only the apparatus shown in Image 3.

Image 3

[Figure: diagram of laboratory apparatus for investigating photosynthesis rate - a boiling tube containing pondweed and sodium hydrogen carbonate (NaHCO3) solution (releases carbon dioxide when added to water), a lamp positioned at a measurable distance using a metre ruler, a spatula, and a stop clock]

[6 QER]

4b
1 mark

It is possible to determine if photosynthesis is taking place by testing a leaf for the presence of starch using iodine solution. Explain why this test is not suitable for determining the rate of photosynthesis.

5a
1 mark

Plant leaves absorb light for photosynthesis.

State the source of the light absorbed by the leaves.

5b
6 marks

Image 1.1 shows a violet plant (Viola riviniana).

Image 1.1

[Figure: photograph of a violet plant (Viola riviniana) growing among other vegetation]

Gareth calculated the area of a leaf from one of the violet plants. This is what he did:

Step 1. He drew an outline of a leaf on a grid as shown in Image 1.2.

Image 1.2

[Figure: grid diagram showing the outline of a leaf drawn over a square grid, used for counting whole squares to estimate leaf area]

Step 2. He counted the number of whole squares inside the outline.

Step 3. He multiplied the total number of whole squares by the area of one square.

(i) Count the number of whole squares inside the outline.

[1]

Number of whole squares inside the outline = ..........................................

(ii) Each square has an area of 0.25 cm2.

Use the formula below to calculate the outline area.

[1]

Outline area = 0.25 × number of whole squares inside the outline.

Outline area = .......................................... cm2

(iii) Counting only the whole squares gave an outline area which was much lower than the actual leaf area.

I. Explain why.

[1]

II. Suggest one improvement to step 2 to get an answer closer to the true value.

[1]

(iv) Gareth decided to test the following hypothesis:

"The leaf area of violet plants in the shade will be greater than that of violet plants in bright light".

He picked one violet plant growing in shade and one growing in bright light.

Gareth used his method to calculate the leaf areas of 10 leaves from each plant.

The mean results are shown in Table 1.3.

Table 1.3

Plant growing in

Mean leaf area (cm2)

shade

1.5

bright light

0.9

I. Calculate the difference in mean leaf area between the two plants.

[1]

Difference = .......................................... cm2

II. Complete the following sentence by underlining the correct word.

[1]

The results show that Gareth's hypothesis is:

invalid / rejected / supported.

5c
2 marks

Explain the advantage of a large leaf area for plants growing in shade.

6a
2 marks

State the word equation for photosynthesis.

6b
9 marks

Students used a computer simulation to investigate how the limiting factors of photosynthesis affect its rate. A screenshot from the simulation is shown in Image 4.1.

Image 4.1

[Figure: Image 4.1 — a screenshot of a photosynthesis simulation showing sliders for temperature (30.0 °C), light intensity (79%) and CO2 concentration (0.2%)]

The simulation allowed the students to carry out investigations into the rate of photosynthesis by adjusting the temperature, light intensity and carbon dioxide concentration the plant received. The rate of photosynthesis was then calculated by measuring the volume of oxygen produced by the plant per hour. The computer then produced a graph of the results as shown in Graph 4.2.

Graph 4.2

[Figure: Graph 4.2 — rate of photosynthesis (volume of oxygen produced per hour) plotted against light intensity (%) for four investigations: Investigation A (30°C, 0.2% CO2), Investigation B (20°C, 0.2% CO2), Investigation C (30°C, 0.1% CO2), Investigation D (20°C, 0.1% CO2), with point X marked on one of the curves]

(i) With reference to limiting factors, explain why the rate of photosynthesis is:

I. lower in investigation C than in investigation A;

[2]

II. higher in investigation C than in investigation D.

[2]

(ii) Suggest how you could increase the rate of photosynthesis in investigation A.

[1]

(iii) State the limiting factor which is controlling the rate of photosynthesis at point X on Graph 4.2.

...................................................................................................

[1]

(iv) The levels were adjusted to the following:

  • light intensity to bright daylight levels

  • CO2 concentration to 0.04%

  • temperature to 80 °C.

I. Suggest how a temperature of 80 °C would affect the rate of photosynthesis and explain your answer.

[2]

II. Suggest why a CO2 concentration of 0.04% was chosen.

[1]

6c
1 mark

Explain why the volume of oxygen produced by the plant per hour can be used as a measurement of the rate of photosynthesis.

7a
7 marks

Tradescantia is a plant whose leaves have green and white areas.

[Figure: Photograph of Tradescantia leaves, showing green and white variegated colouring]

In the experiment below, a Tradescantia plant is used in an investigation to demonstrate three factors, A, B and C, needed for photosynthesis.

[Figure: Diagram of three experimental setups using Tradescantia plants. Setup A: a leaf partly covered with black paper, with the appearance after a starch test (A1) showing blue-black areas except where covered by black paper. Setup B: a Tradescantia plant with cotton wool and a flask of 20% sodium hydroxide solution, with the appearance after a starch test (B1) showing a green area appearing white/no blue-black colour, compared to a blue-black area before the starch test. Setup C: a leaf tested for starch (C1) showing a blue-black area (green part) and a brown area (white part) before the starch test]

(i) State the factor needed for photosynthesis, which is being demonstrated in each of the following:

A ........................................................................................

B ........................................................................................

C ........................................................................................

[3]

(ii) How could the experiment in flasks B and B1 be improved? Explain your answer.

[2]

(iii) Before the apparatus was set up, the Tradescantia plant was kept in a dark cupboard for 48 hours. Explain the reason for this.

[2]

7b
3 marks

The diagram below shows the stages in testing a leaf for starch.

[Figure: Diagram showing four numbered stages of the starch test procedure for a Tradescantia leaf: (1) leaf placed in boiling liquid, (2) leaf placed in a second liquid with the Bunsen burner removed, (3) leaf rinsed in a third liquid on a white tile, (4) leaf covered with a fourth liquid to observe the colour change]

Name the liquids 1, 2, 3 and 4 shown in the diagram.

1 ........................................................................................

2 ........................................................................................

3 ........................................................................................

4 ........................................................................................

8
3 marks

An incomplete diagram of a plant leaf cell is shown in Image 1.1.

Image 1.1

[Figure: Image 1.1 — an incomplete diagram of a plant leaf cell, showing labelled structure X (chloroplast) but missing the cell wall]

(i) Complete Image 1.1 by drawing the cell wall.

[1]

(ii) Structure X contains chlorophyll and absorbs light.

I. Complete the following sentence by underlining the correct term in the brackets.

Structure X is a (vacuole / chloroplast / mitochondrion).

[1]

II. State the name of the process that uses the light absorbed by structure X.

..................................................................................

[1]

9
1 mark

Seaweeds (marine algae) contain large amounts of iodine in their tissues. The iodine is obtained from the seawater in which they live and is needed to maintain healthy growth of the plant. The iodine content of seawater is about 0.055 mg / kg. Some of the largest seaweeds are the kelps that live in kelp ‘forests’ in shallow seas and oceans where light can penetrate. Some kelp species are over 80 m in length and can grow 0.5 m in a day.

[Figure: Photo showing part of a kelp 'forest' with a sub-aqua diver swimming among tall kelp fronds]

State the reason why kelp only grows in seawater where sunlight can penetrate.

10a
3 marks

In a classroom exercise some students used a computer simulation to investigate the factors affecting the rate of photosynthesis. A well-watered plant was placed inside a transparent sealed container with a high intensity lamp above the plant. The air in the airtight container was continually monitored in order to measure the rate of O2 production.

  • The light intensity of the lamp could be varied between 0–100%.

  • The CO2 concentration of the air inside the sealed container could be varied between 0–1000 ppm.

  • The temperature inside the sealed container could be varied between 0–50 °C.

[Figure: Diagram of the sealed container apparatus, showing a lamp above a plant, an oxygen meter monitoring O2 production, and dials for light intensity (0-100%), CO2 concentration (0-1000 ppm), and temperature (0-50°C)]

The table below shows the results obtained by one of the students.

Reading number

Light intensity (%)

Temperature (°C)

CO2 concentration (ppm)

O2 production (cm3/h)

1

0

10

0

0.0

2

20

10

200

3.1

3

40

10

200

3.1

4

40

10

400

3.1

5

40

20

400

34.7

6

60

20

400

41.7

7

80

20

400

41.7

8

100

25

400

41.7

9

100

25

600

47.3

Explain how oxygen production can be used as a measure of the rate of photosynthesis.

10b
2 marks

Explain why oxygen production remains at 3.1 cm3/h for readings 2, 3 and 4.

10c
2 marks

Identify the limiting factor for readings 6, 7, 8 and 9 and explain your answer.

10d
1 mark

State why the container must be sealed.

10e
2 marks

Sian and Dafydd are students who carried out the computer simulation. Sian suggested that they try to set up a 'live version' of the apparatus in the laboratory. Dafydd said that if they did this, a problem could arise which would affect the validity of the experiment.

Suggest the problem that may arise and how this could be solved.