Exam code: 3430
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Give three ways in which living organisms show variety.
Size, e.g. bacteria are microscopic while blue whales are huge
Features, e.g. plant cells can photosynthesise but animal cells cannot
Complexity, e.g. single-celled organisms are simpler than multicellular ones

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Into which two broad groups are plants divided?
Flowering plants, e.g. sunflowers, grasses and oak trees
Non-flowering plants, e.g. mosses and ferns
Into which two broad groups are animals divided?
Vertebrates, which have a backbone, e.g. mammals and birds
Invertebrates, which do not, e.g. insects and arachnids
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Give three ways in which living organisms show variety.
Size, e.g. bacteria are microscopic while blue whales are huge
Features, e.g. plant cells can photosynthesise but animal cells cannot
Complexity, e.g. single-celled organisms are simpler than multicellular ones
Into which two broad groups are plants divided?
Flowering plants, e.g. sunflowers, grasses and oak trees
Non-flowering plants, e.g. mosses and ferns
Into which two broad groups are animals divided?
Vertebrates, which have a backbone, e.g. mammals and birds
Invertebrates, which do not, e.g. insects and arachnids
Define classification.
The process of putting organisms into groups on the basis of their similar features or DNA.
On what two bases can organisms be classified?
Similar morphological features, i.e. their physical appearance
DNA analysis, grouping organisms with similar DNA sequences
Why is classification important?
It aids scientific understanding of the variety of life.
It reveals the relationships between organisms, showing which species share a common ancestor.
Name the five kingdoms.
Bacteria
Protists
Plants
Fungi
Animals
Describe the scientific system for naming species.
Each species is given a two-part Latin name, taken from its genus and species, e.g. Homo sapiens.
Why are scientific names used instead of common names?
Scientific names are universal, so scientists worldwide use the same name for each species.
Common names vary between languages and regions, which causes confusion.
In the name Canis lupus, what does each part refer to?
Canis is the genus
lupus is the species
Animals with a backbone are called ________.
Animals with a backbone are called vertebrates.
The scientific name of a species is made up of its genus and its ________.
The scientific name of a species is made up of its genus and its species.
True or False?
A moss is an example of a non-flowering plant.
True.
Mosses and ferns are non-flowering plants because they do not produce flowers.
True or False?
Two organisms that look similar must always be closely related.
False.
Appearance alone can be misleading. DNA analysis gives a more reliable picture of how closely species are related.
Give one advantage of the two-part naming system over older naming systems.
Older names could be extremely long and unwieldy.
A two-part name is short and simple, allowing easy identification.
Define adaptation.
A feature of an organism that aids its survival in its environment.
Define morphological adaptation.
A physical feature of an organism that aids its survival.
Define behavioural adaptation.
Something an organism does that aids its survival.
Explain how the Arctic fox's short, rounded ears and thick fur aid survival.
They reduce heat loss to the very cold environment, helping the fox maintain its body temperature.
Explain how a cactus's spines aid survival.
Spines give a much reduced leaf surface area, so less water is lost by transpiration in a dry desert environment.
Explain how a dolphin's streamlined body shape aids survival.
It improves swimming efficiency, so the dolphin can move faster and use less energy.
Explain how being nocturnal aids the survival of desert rodents.
Staying in underground burrows during the heat of the day prevents them from overheating and losing too much water.
Explain how hunting in groups aids the survival of lions.
There is an increased chance of catching prey, so the pride is more likely to have enough food.
A ________ adaptation is a physical feature of an organism that aids survival.
A morphological adaptation is a physical feature of an organism that aids survival.
Hunting in groups is an example of a ________ adaptation.
Hunting in groups is an example of a behavioural adaptation.
True or False?
A penguin huddling with others to keep warm is a morphological adaptation.
False.
Huddling is something the penguin does, so it is a behavioural adaptation.
True or False?
Simply naming an adaptive feature is enough to gain full marks in an exam.
False.
You must state the feature and then explain how it aids survival. Many marks are lost by omitting the explanation.
Fennec foxes have very large ears. Explain how this aids survival in the desert.
The large surface area allows more heat to be lost to the surroundings, helping the fox stay cool in a hot environment.
How should you structure an answer about an adaptation?
State the feature, then explain how it aids survival in that particular environment.
Name four resources animals need from their environment.
Food
Water
Mates
Territory containing those resources
Name four resources plants need from their environment.
Water
Light
Minerals
Space to grow
Define intraspecific competition.
Competition for resources between individuals of the same species.
Define interspecific competition.
Competition for resources between individuals of different species.
How does competition affect the size of a population?
Competition for resources, whether within a species or with another species, limits the maximum size the population can reach.
How does predation affect the size of a population?
Predators increase the death rate, slowing population growth.
A lack of predators may allow a population to grow rapidly.
Why does disease have a greater effect in a large, crowded population?
Infection spreads more quickly between individuals in crowded conditions.
How can pollution affect the size of a population?
Pollution can kill individuals directly and alter the structure of the ecosystem, e.g. by removing a species' food source or its predators.
Competition between grey squirrels and red squirrels is an example of ________ competition.
Competition between grey squirrels and red squirrels is an example of interspecific competition.
Competition between individual grey squirrels for the same food is an example of ________ competition.
Competition between individual grey squirrels for the same food is an example of intraspecific competition.
True or False?
A population with no predators will keep growing without limit.
False.
It may grow rapidly at first, but competition for resources and disease will eventually limit its size.
True or False?
Organisms only compete with members of their own species.
False.
They also compete with different species that need the same resources. This is interspecific competition.
Name four factors that can affect the size of a population.
Competition
Predation
Disease
Pollution
Explain why heavy metal pollution can indirectly increase a prey population.
Heavy metals accumulate most in top predators.
If predator numbers fall as a result, there is less predation, so the prey population can increase.
Define biodiversity.
The variety of different species and the numbers of individuals within those species in an area.
How is the biodiversity of a habitat assessed?
By measuring:
The number of different species present
The number of individuals of each species
Why is a habitat with high biodiversity better able to cope with change?
Many species interact, so no single species depends entirely on one other.
If one species declines, others can take its place in the food web.
Give three reasons why biodiversity is important to humans.
Any three of:
A plentiful and reliable food supply
A reliable supply of biological resources, e.g. timber
Potential medicines are not lost before being discovered
Well-being benefits of spending time in nature
What is CITES and how does it protect biodiversity?
The Convention on International Trade in Endangered Species.
It is an agreement between governments to prevent the capture and sale of endangered plant and animal species.
How do SSSIs and national parks protect biodiversity?
They are protected areas in which human activities are regulated, protecting the species and ecosystems inside their borders.
How do captive breeding programmes help conservation?
Zoos carry out carefully controlled breeding to increase numbers while maintaining genetic health.
Offspring may stay in the programme or be released into the wild.
What is a seed bank and why is it useful?
A store of seeds from many plant species kept under carefully controlled conditions.
It means plant species can be saved even if their natural habitats are lost.
What is the purpose of a local biodiversity action plan?
To identify at-risk species in an area and set out the steps needed to protect them and their habitats.
________ is the variety of different species and the numbers of individuals within them in an area.
Biodiversity is the variety of different species and the numbers of individuals within them in an area.
________ banks store the sex cells of endangered animals to protect their genetic material.
Sperm banks store the sex cells of endangered animals to protect their genetic material.
True or False?
A habitat with an even spread of numbers across many species is more biodiverse.
True.
Both the number of species and the evenness of their numbers contribute to biodiversity.
True or False?
Global biodiversity is currently under threat.
True.
An increasing human population and its demand for resources is putting biodiversity worldwide under pressure.
In a biodiverse food web, why would losing one prey species not starve the predators?
The predators have other food sources available.
This is why a food web with many species is resilient to change.
Give one issue surrounding the use of legislation to protect endangered species.
Laws must be enforced to work, which is difficult and costly across international borders.
Restrictions may also conflict with local people's livelihoods or a country's economic development.
Define sampling.
The process of collecting a series of small data sets that can be used to represent an entire population.
What two things can sampling in a habitat be used to assess?
Abundance: how many organisms or species are present
Distribution: where a species is found within the habitat
Why is sampling used rather than counting every organism?
In large and complex habitats it is not possible to find, identify and count every organism present.
State the two requirements for a sample to be representative.
Sample sites must be chosen randomly, to avoid bias
Enough samples must be taken; more samples give more representative results
Define quadrat.
A square frame placed within an area being studied in order to take a sample.
Describe how to place quadrats randomly in a habitat.
Lay out two tape measures at the edges of the habitat like a pair of graph axes to create a grid.
Use a random number generator to choose two numbers as grid coordinates.
Place the quadrat at that point and take a measure of abundance. Repeat many times.
Why must quadrats be placed randomly?
To avoid bias, e.g. a student choosing a spot where the organisms look easier to count.
Give three measures of abundance that can be taken from a quadrat.
The number of species within the quadrat
The number of individuals of a particular species
The percentage cover of a species
When is percentage cover a more useful measure than counting individuals?
When individual organisms cannot easily be distinguished, for example grass.
Define transect.
A line laid out across a habitat, used to measure the effect of an environmental factor on distribution along a gradient.
Describe how quadrats are used along a transect.
Lay a tape measure across the habitat along the chosen gradient.
Place the quadrat at set intervals, e.g. every 1 or 5 metres.
Take a measure of abundance at each point, and repeat to get a representative sample.
Quadrats should be placed ________ within a habitat to avoid bias.
Quadrats should be placed randomly within a habitat to avoid bias.
A ________ is used to study the distribution of organisms along an environmental gradient.
A transect is used to study the distribution of organisms along an environmental gradient.
True or False?
All quadrats are the same size.
False.
Quadrat size depends on the organisms studied. A 1 m² quadrat suits small plants, while a 400 m² quadrat may be used for trees.
True or False?
Taking more samples generally makes the results more representative.
True.
A larger number of samples is more likely to reflect the habitat as a whole, making conclusions more valid.
Why is capture/recapture used instead of quadrat sampling for some species? (Higher Tier Only)
Quadrats only work for sessile organisms that stay in one place.
Capture/recapture is used to estimate the population of motile animals that move around.
Describe the capture/recapture method. (Higher Tier Only)
Capture a sample of the population and mark each animal using a non-harmful method.
Release them and allow time for them to mix with the rest of the population.
Capture a second sample and count how many are marked and unmarked.
Use the equation to estimate the population size.
State the equation used to estimate population size in capture/recapture. (Higher Tier Only)
N = (n₁ × n₂) ÷ m₂
N = population estimate
n₁ = number marked and released in the first sample
n₂ = total number in the second sample
m₂ = number of marked individuals in the second sample
236 insects are marked and released. A second sample of 244 contains 71 marked. Estimate the population. (Higher Tier Only)
N = (236 × 244) ÷ 71
N = 57 584 ÷ 71
N = 811
Why must the marked animals be left for a period before the second sample is taken? (Higher Tier Only)
So they have time to mix evenly with the rest of the population, otherwise the second sample would not be representative.
State two assumptions made when using capture/recapture. (Higher Tier Only)
The population stays the same size during the study, with no births, deaths or migration
The marking does not affect survival, e.g. by making individuals easier for predators to see
Why must the marking method be non-harmful and non-toxic? (Higher Tier Only)
So the marking does not change the animal's chance of survival, which would make the population estimate invalid.
Capture/recapture is used to estimate the population size of ________ animals. (Higher Tier Only)
Capture/recapture is used to estimate the population size of motile animals.
Capture/recapture is also known as the mark-________-capture technique. (Higher Tier Only)
Capture/recapture is also known as the mark-release-capture technique.
True or False?
Capture/recapture assumes no animals are born or die during the study. (Higher Tier Only)
True.
The method assumes the population stays the same size between the two samples. Births, deaths or migration would make the estimate unreliable.
True or False?
Finding very few marked individuals in the second sample suggests a large population. (Higher Tier Only)
True.
A small proportion of marked animals means the marked individuals are diluted among many unmarked ones, so the population must be large.
Suggest why the estimate would be too high if some marked animals died. (Higher Tier Only)
Fewer marked animals would be recaptured, making m₂ smaller.
Dividing by a smaller number gives a larger population estimate than the true value.
Give an example of a suitable marking method. (Higher Tier Only)
A small amount of non-toxic waterproof paint applied to the underside of the animal.
Name three factors that can affect the distribution and abundance of a species.
Any three of:
Light intensity
Competition from other species
The presence of a feature such as a road or stream
Disturbance, e.g. grazing or mowing
Describe how to investigate the effect of mowing on daisy abundance on a school field.
Lay two tape measures at right angles on the mowed area to create grid axes.
Use a random number generator for two grid coordinates and place a 1 m² quadrat there.
Count the daisies in the quadrat, then repeat for at least 10 quadrats.
Repeat the whole process on an unmowed section and compare the estimates.
Why are random number generators used to position the quadrats?
To ensure sample sites are chosen at random, which avoids bias and makes the results more likely to represent the area accurately.
What rule is used for plants lying on the edge of a quadrat?
Include plants that fall more than half within the quadrat.
State the equation used to estimate a population from quadrat data.
Population estimate = number of individuals in sample × (total area ÷ sample area)
56 daisies are counted in fifteen 1 m² quadrats on a 400 m² field.
Estimate the population.
Population estimate = 56 × (400 ÷ 15)
Population estimate = 1 493.3
Population estimate = 1 493 daisies
State the independent and dependent variables in this investigation.
Independent variable: whether or not the field has been mowed
Dependent variable: the estimated daisy population for each field
Give three control variables for this investigation.
Any three of:
Time of year the samples are collected
Soil type
Light intensity
Level of disturbance, e.g. from students using the field
Why should at least 10 quadrats be sampled rather than just one or two?
A larger number of samples is more representative of the whole area, making any conclusion drawn from the data valid.
How could you identify a daisy plant that is not in flower?
Use a field identification guide to recognise the shape of the leaves.
State one hazard, risk and control measure for this fieldwork.
Hazard: plant thorns and stings
Risk: cuts or stings to the skin
Control measure: avoid touching plants while counting and learn to identify harmful species
A 1 m² ________ is used to sample the daisies at each grid coordinate.
A 1 m² quadrat is used to sample the daisies at each grid coordinate.
True or False?
Some control variables in fieldwork may be outside the student's control.
True.
Factors such as soil type and light intensity cannot always be controlled, so their possible effect must be considered when drawing conclusions.
True or False?
Choosing where to place a quadrat by eye gives a fair sample.
False.
Choosing by eye introduces bias. Quadrat positions must be chosen using random coordinates.
Define biological control agent.
A living organism used to limit the population of a pest species, keeping its numbers to a minimum.
Name three types of organism used as biological control agents.
Predators
Parasites
Pathogens
What is the main advantage of biological control over chemical pesticides?
It reduces reliance on chemical pesticides, which cause pollution and toxicity in food chains.
Explain how parasitic wasps act as biological control agents.
They lay their eggs inside the bodies of the pest species.
The pests die when the wasp eggs hatch, reducing the pest population.
Define alien species.
A species introduced to a region where it does not naturally occur. Also known as an invasive species.
Give two ways alien species come to be introduced to new regions.
Deliberately by humans, e.g. as a pet or garden plant
Accidentally, e.g. travelling attached to the hull of a ship
Why did introducing cane toads to Australia cause problems?
Cane toads are toxic, so they poison native predators that eat them.
The lack of suitable predators meant their population exploded.
How has the Asian ladybird damaged European ladybird populations?
The Asian species is more aggressive and will prey on the native species.
Why is Rhododendron ponticum a problem in UK woodlands?
It grows fast and outcompetes native plants for light and space.
Its fallen leaves also produce leaf litter that is toxic to many other species.
Why must potential biological control agents be researched before release?
To check their effect on non-target native species, and to work out how the agent itself could be controlled if problems arise.
A species introduced to a region where it does not naturally occur is called an ________ species.
A species introduced to a region where it does not naturally occur is called an alien species.
Biological control agents are living organisms used to limit the population of a ________ species.
Biological control agents are living organisms used to limit the population of a pest species.
True or False?
The cane toad was originally introduced to Australia as a biological control agent.
True.
It was introduced in the 1930s to control pests, but became a serious invasive species itself.
True or False?
Biological control is always safer than using pesticides.
False.
It avoids chemical pollution, but a poorly researched control agent can become an invasive species and cause serious ecological harm.
Suggest why a species may spread rapidly in a new region.
It has no natural predators there and may outcompete native species for food, light or space.
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