Required Practical: Ecosystems (AQA GCSE Combined Science: Trilogy: Biology): Revision Note

Exam code: 8464

Lára Marie McIvor

Written by: Lára Marie McIvor

Reviewed by: Dr Natalie Lawrence

Updated on

RP9: Investigating ecosystems

  • Aim:

    • to measure the population size of a common species in a habitat and use sampling techniques to investigate the effect of a factor on the distribution of this species

  • Proceedure:

    • use a quadrat to estimate the population size of a plant species in a survey area

    • use a transect line and a quadrat to investigate the effect of a factor on the number of plants in a survey area

Measuring population size

  • Population size can be measured by taking random samples within a habitat

    • Sampling reduces the time spent counting organisms

    • The sample represents the population as a whole, so must be large enough, and must be unbiased

  • Samples are taken using quadrats, which are placed randomly within the habitat to avoid bias, e.g. so that students don't just sample the closest part of the habitat

    • Randomness is achieved using a grid and random number generator

Diagram showing the first three steps for estimating plant population size using quadrats. A 10 m × 10 m survey area is marked out using tape measures. Randomly generated coordinates are used to position a 1 m × 1 m quadrat; an example places the quadrat 4 m along the x-axis and 5 m along the y-axis. The number of the chosen plant species within the quadrat is then counted; the example quadrat contains three dandelions.
Using random quadrat sampling to estimate the abundance of a plant species
Worked example showing results from 10 randomly placed 1 m × 1 m quadrats. The numbers of dandelions recorded are 3, 4, 2, 1, 0, 0, 2, 5, 3 and 1, giving a total of 21. Population size is estimated using total area divided by area sampled, multiplied by the total number of organisms counted. For a 100 m² survey area with 10 m² sampled, the estimated dandelion population is 100 ÷ 10 × 21 = 210.
Using quadrat results to estimate the total population size of a plant species.

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Estimating population size method:

  1. Use two tape measures to lay out a survey area (e.g. 10 m × 10 m) in the chosen habitat, such as the school field

  2. Use a random number generator to create a set of coordinates to place the first quadrat. E.g. a 4 and a 5 would mean to place the quadrat 4 m along the x-axis and 5 m along the y-axis

  3. Count the number of the chosen plant species (e.g. dandelions) that are found within this quadrat

  4. Record this number in a results table and repeat steps 1–3 to record the number of the chosen plant species in all 10 quadrats

  5. Estimate the population of the survey area using the equation:

estimated population size = (total areaarea sampled) × total number counted

Worked Example

A student carried out a quadrat survey of their school field and counted the number of dandelions in each quadrat.

Each quadrat is 1 m × 1 m and 10 quadrats were placed. Total survey area was 10 m × 10 m.

They obtained the results below:

Quadrat

Number of dandelions

1

3

2

4

3

2

4

1

5

0

6

0

7

2

8

5

9

3

10

1

Total

21

Answer:

estimated population size = (total area ÷ area sampled) × total number of dandelions counted

= (100 ÷ 10) × 21

estimated population of dandelions = 210

Investigating the effect of a factor on species distribution

  • Species are not usually evenly distributed throughout a habitat, but can be affected by factors in the environment, e.g. the presence of a river, or a large tree

  • Sampling using a transect allows the effect of a particular factor on the distribution of a species to be assessed

Method:

  1. Set the transect up through the area of investigation (e.g. a 30 m tape measure is placed up a hillside)

  2. Place a quadrat at equal intervals along the transect (e.g. every 5 m)

  3. Record the number of the chosen plant species inside each quadrat

  4. Record the abiotic factor (e.g. altitude) at each quadrat

  5. Record the results in a table

  6. Plot the data in a graph and describe any relationship that can be observed

Diagram showing a 30 m transect running down a hillside, with seven quadrats placed at 5 m intervals. The number of dandelions and altitude are recorded at each quadrat. Results show distances along the transect of 0, 5, 10, 15, 20, 25 and 30 m; dandelion counts of 84, 66, 62, 45, 30, 30 and 13; and corresponding altitudes of 2, 4, 6, 8, 10, 12 and 14 m.
Scatter graph showing the number of dandelions recorded in quadrats against altitude. Altitude is the independent variable on the x-axis and number of dandelions is the dependent variable on the y-axis. The plotted points decrease from about 84 dandelions at 0 m to 0 at 14 m, with a downward-sloping line of best fit. Labels identify good graphing practice, including sensible scales, labelled axes with units, accurate plotting and using at least half the available space. The relationship identified is that as altitude increases, the number of dandelions decreases.
A transect can be used to assess the impact of altitude on the distribution of a plant species

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Lára Marie McIvor

Author: Lára Marie McIvor

Expertise: Content Creator

Lára graduated from Oxford University in Biological Sciences and has now been a science tutor working in the UK for several years. Lára has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning.

Dr Natalie Lawrence

Reviewer: Dr Natalie Lawrence

Expertise: Content Writer

Natalie has a MCantab, Masters and PhD from the University of Cambridge and has tutored biosciences for 14 years. She has written two internationally-published nonfiction books, produced articles for academic journals and magazines, and spoken for TEDX and radio.