Required Practical: Ecosystems (AQA GCSE Combined Science: Trilogy: Biology): Revision Note
Exam code: 8464
RP9: Investigating ecosystems
Aim:
to measure the population size of a common species in a habitat and use sampling techniques to investigate the effect of a factor on the distribution of this species
Proceedure:
use a quadrat to estimate the population size of a plant species in a survey area
use a transect line and a quadrat to investigate the effect of a factor on the number of plants in a survey area
Measuring population size
Population size can be measured by taking random samples within a habitat
Sampling reduces the time spent counting organisms
The sample represents the population as a whole, so must be large enough, and must be unbiased
Samples are taken using quadrats, which are placed randomly within the habitat to avoid bias, e.g. so that students don't just sample the closest part of the habitat
Randomness is achieved using a grid and random number generator


Estimating population size method:
Use two tape measures to lay out a survey area (e.g. 10 m × 10 m) in the chosen habitat, such as the school field
Use a random number generator to create a set of coordinates to place the first quadrat. E.g. a 4 and a 5 would mean to place the quadrat 4 m along the x-axis and 5 m along the y-axis
Count the number of the chosen plant species (e.g. dandelions) that are found within this quadrat
Record this number in a results table and repeat steps 1–3 to record the number of the chosen plant species in all 10 quadrats
Estimate the population of the survey area using the equation:
Worked Example
A student carried out a quadrat survey of their school field and counted the number of dandelions in each quadrat.
Each quadrat is 1 m × 1 m and 10 quadrats were placed. Total survey area was 10 m × 10 m.
They obtained the results below:
Quadrat | Number of dandelions |
|---|---|
1 | 3 |
2 | 4 |
3 | 2 |
4 | 1 |
5 | 0 |
6 | 0 |
7 | 2 |
8 | 5 |
9 | 3 |
10 | 1 |
Total | 21 |
Answer:
estimated population size = (total area ÷ area sampled) × total number of dandelions counted
= (100 ÷ 10) × 21
estimated population of dandelions = 210
Investigating the effect of a factor on species distribution
Species are not usually evenly distributed throughout a habitat, but can be affected by factors in the environment, e.g. the presence of a river, or a large tree
Sampling using a transect allows the effect of a particular factor on the distribution of a species to be assessed
Method:
Set the transect up through the area of investigation (e.g. a 30 m tape measure is placed up a hillside)
Place a quadrat at equal intervals along the transect (e.g. every 5 m)
Record the number of the chosen plant species inside each quadrat
Record the abiotic factor (e.g. altitude) at each quadrat
Record the results in a table
Plot the data in a graph and describe any relationship that can be observed


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