Required Practical: Ecosystems (AQA GCSE Biology): Revision Note

Exam code: 8461

Lára Marie McIvor

Written by: Lára Marie McIvor

Reviewed by: Dr Natalie Lawrence

Updated on

RP9: Investigating ecosystems

Aim:

  • Measure the population size of a common species in a habitat

  • Use sampling techniques to investigate the effect of a factor on the distribution of this species

Sampling

  • Population size can be measured by taking random samples within a habitat

    • Sampling reduces the time spent counting organisms

    • The sample represents the population as a whole, so must be large enough, and must be unbiased

Method: estimating population size

  • Use a quadrat to estimate the population size of a plant species in a survey area as follows:

    1. Lay out two tape measures at the edges of a habitat like a pair of graph axes; this creates a grid

    2. Use a random number generator to choose two numbers to use as grid co-ordinates

      • The quadrat is placed at this location on the grid

    3. Take a measure of abundance for your chosen species, e.g.

      • The number of individuals within the quadrat

      • The percentage cover within the quadrat

    4. Record abundance in a suitable results table

    5. Repeat steps 2-4 a number of times in order to obtain a representative sample

    6. Estimate the population of the survey area using the equation:

    estimated population size = (total areaarea sampled) × number of individuals counted

Diagram showing the first three steps for estimating plant population size using quadrats. A 10 m × 10 m survey area is marked out using tape measures. Randomly generated coordinates are used to position a 1 m × 1 m quadrat; an example places the quadrat 4 m along the x-axis and 5 m along the y-axis. The number of the chosen plant species within the quadrat is then counted; the example quadrat contains three dandelions.

Worked Example

A student carried out a quadrat survey of their school field and counted the number of dandelions in each quadrat.

Each quadrat is 1 m × 1 m and 10 quadrats were placed. Total survey area was 10 m × 10 m.

They obtained the results below:

Quadrat

Number of dandelions

1

3

2

4

3

2

4

1

5

0

6

0

7

2

8

5

9

3

10

1

Total

21

Estimate the size of the dandelion population.

Answer:

Step 1: determine the total sample area

  • Total survey area was 10 m × 10 m

10 x 10 = 100 m2

Step 2: determine the area sampled

  • 10 quadrats are recorded in the table

  • Each quadrat is 1 m x 1 m = 1 m2

10 x 1 = 10 m2

Step 3: Determine the number of dandelions counted

  • Provided in the table = 21 dandelions

Step 4: enter into population size equation

estimated population size = (total area ÷ area sampled) × total number of dandelions counted

= (100 ÷ 10) × 21

= 210

Method: effect of a factor on species distribution

  • The effect of a factor on species distribution can be studied using a transect as follows:

    1. Set the a tape measure across the gradient to be studied, e.g. ascending up the side of a hill

    2. Place a quadrat at equal intervals along the transect, e.g. every 5 m

    3. Record the number of the chosen plant species inside each quadrat

    4. Record the abiotic factor, e.g. altitude, for each quadrat

    5. Record the results in a table

    6. Plot the data in a graph and describe any relationship that can be observed

Diagram showing a 30 m transect running down a hillside, with seven quadrats placed at 5 m intervals. The number of dandelions and altitude are recorded at each quadrat. Results show distances along the transect of 0, 5, 10, 15, 20, 25 and 30 m; dandelion counts of 84, 66, 62, 45, 30, 30 and 13; and corresponding altitudes of 2, 4, 6, 8, 10, 12 and 14 m.
Scatter graph showing the number of dandelions recorded in quadrats against altitude. Altitude is the independent variable on the x-axis and number of dandelions is the dependent variable on the y-axis. The plotted points decrease from about 84 dandelions at 0 m to 0 at 14 m, with a downward-sloping line of best fit. Labels identify good graphing practice, including sensible scales, labelled axes with units, accurate plotting and using at least half the available space. The relationship identified is that as altitude increases, the number of dandelions decreases.
A transect can be used to assess the impact of altitude on the distribution of a plant species

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Lára Marie McIvor

Author: Lára Marie McIvor

Expertise: Content Creator

Lára graduated from Oxford University in Biological Sciences and has now been a science tutor working in the UK for several years. Lára has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning.

Dr Natalie Lawrence

Reviewer: Dr Natalie Lawrence

Expertise: Content Writer

Natalie has a MCantab, Masters and PhD from the University of Cambridge and has tutored biosciences for 14 years. She has written two internationally-published nonfiction books, produced articles for academic journals and magazines, and spoken for TEDX and radio.