Required Practical: Ecosystems (AQA GCSE Biology): Revision Note
Exam code: 8461
RP9: Investigating ecosystems
Aim:
Measure the population size of a common species in a habitat
Use sampling techniques to investigate the effect of a factor on the distribution of this species
Sampling
Population size can be measured by taking random samples within a habitat
Sampling reduces the time spent counting organisms
The sample represents the population as a whole, so must be large enough, and must be unbiased
Method: estimating population size
Use a quadrat to estimate the population size of a plant species in a survey area as follows:
Lay out two tape measures at the edges of a habitat like a pair of graph axes; this creates a grid
Use a random number generator to choose two numbers to use as grid co-ordinates
The quadrat is placed at this location on the grid
Take a measure of abundance for your chosen species, e.g.
The number of individuals within the quadrat
The percentage cover within the quadrat
Record abundance in a suitable results table
Repeat steps 2-4 a number of times in order to obtain a representative sample
Estimate the population of the survey area using the equation:

Worked Example
A student carried out a quadrat survey of their school field and counted the number of dandelions in each quadrat.
Each quadrat is 1 m × 1 m and 10 quadrats were placed. Total survey area was 10 m × 10 m.
They obtained the results below:
Quadrat | Number of dandelions |
|---|---|
1 | 3 |
2 | 4 |
3 | 2 |
4 | 1 |
5 | 0 |
6 | 0 |
7 | 2 |
8 | 5 |
9 | 3 |
10 | 1 |
Total | 21 |
Estimate the size of the dandelion population.
Answer:
Step 1: determine the total sample area
Total survey area was 10 m × 10 m
10 x 10 = 100 m2
Step 2: determine the area sampled
10 quadrats are recorded in the table
Each quadrat is 1 m x 1 m = 1 m2
10 x 1 = 10 m2
Step 3: Determine the number of dandelions counted
Provided in the table = 21 dandelions
Step 4: enter into population size equation
estimated population size = (total area ÷ area sampled) × total number of dandelions counted
= (100 ÷ 10) × 21
= 210
Method: effect of a factor on species distribution
The effect of a factor on species distribution can be studied using a transect as follows:
Set the a tape measure across the gradient to be studied, e.g. ascending up the side of a hill
Place a quadrat at equal intervals along the transect, e.g. every 5 m
Record the number of the chosen plant species inside each quadrat
Record the abiotic factor, e.g. altitude, for each quadrat
Record the results in a table
Plot the data in a graph and describe any relationship that can be observed


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