Osmosis (AQA GCSE Biology): Revision Note

Exam code: 8461

Lára Marie McIvor

Written by: Lára Marie McIvor

Reviewed by: Dr Natalie Lawrence

Updated on

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Principles of osmosis

The process of osmosis

Diagram of osmosis showing net movement of water through a partially permeable membrane into a sucrose-filled cell, while sucrose molecules cannot cross.
Osmosis occurs when two solutions are separated by a partially permeable membrane

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  • The movement of water into and out of cells occurs by osmosis

  • Osmosis in biology is the diffusion of water molecules from a dilute solution to a concentrated solution through a partially permeable membrane

  • Osmosis is just a special type of diffusion, so water molecules move about randomly just like any other particle that can diffuse

  • Water will always move from a more dilute solution to a more (solute) concentrated solution to even up the concentration of water molecules on each side of the membrane

Effect of solution concentration on water potential

  • A dilute solution has a high concentration of water molecules, a high water potential, and a low concentration of solute molecules (lots of water, not a lot of solute)

  • A concentrated solution has a low concentration of water molecules. a low water potential, and a high concentration of solute molecules (lots of solute, not a lot of water)

 Water potential gradient and osmosis

Diagram explaining osmosis: water moves from dilute to concentrated sugar solution across a partially permeable membrane, down the water potential gradient.
Always identify which solution is more concentrated, and which is more dilute, to determine the direction of movement of water by osmosis

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Examiner Tips and Tricks

Osmosis refers only to the movement of water molecules, so if in an exam you are talking about the movement of water, make sure you mention osmosis as this will often earn you a mark.

The effect of osmosis on plant tissue

  • The movement of water by osmosis can easily be observed by placing two similar pieces of plant tissue into solutions with differing concentrations

Method to observe the effects of osmosis

Diagram showing radish pieces in dilute (0.1 mol/dm2) and concentrated (1 mol/dm2) sugar solutions, gaining or losing water by osmosis and changing length and mass over four hours. The radish piece in a dilute solution gains size as it has gained water by osmosis, while the piece in a concentrated solution shrinks as it has lost water by osmosis.
Potatoes are usually used in osmosis experiments to show how the concentration of a solution affects the movement of water, but radishes can be used too

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  • If the plant tissue gains mass:

    • water must have moved into the plant tissue from the solution surrounding it by osmosis

    • the solution surrounding the tissue is more dilute than the plant tissue (which is more concentrated)

  • If plant tissue loses mass:

    • water must have moved out of the plant tissue into the solution surrounding it by osmosis

    • the solution surrounding the tissue is more concentrated than the plant tissue (which is more dilute)

  • If there is no overall change in mass:

    • there has been no net movement of water as the concentration in both the plant tissue and the solution surrounding it must be equal

    • remember that water will still be moving into and out of the plant tissue, but there wouldn’t be any net movement in this case

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Lára Marie McIvor

Author: Lára Marie McIvor

Expertise: Content Creator

Lára graduated from Oxford University in Biological Sciences and has now been a science tutor working in the UK for several years. Lára has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning.

Dr Natalie Lawrence

Reviewer: Dr Natalie Lawrence

Expertise: Content Writer

Natalie has a MCantab, Masters and PhD from the University of Cambridge and has tutored biosciences for 14 years. She has written two internationally-published nonfiction books, produced articles for academic journals and magazines, and spoken for TEDX and radio.