Factors that Affect Diffusion (AQA GCSE Biology): Revision Note
Exam code: 8461
Diffusion: Factors that affect rate
The following factors affect the rate of diffusion:
Difference in concentrations (concentration gradient)
The greater the difference in concentration between two regions, the faster the overall rate of diffusion.
Temperature
The higher the temperature, the more kinetic (movement) energy the particles of that substance will have.
They will move/spread faster compared to when at a lower temperature, when they have less kinetic energy.
Surface area of a membrane separating two regions
A membrane with a greater surface area will have a greater rate of diffusion across it.
Think of there being more ‘entry or exit points’ for particles to cross.
Examiner Tips and Tricks
Remember that diffusion is a passive process, so when it occurs in a living organism, the cells of that organism do not provide the particles involved with energy to diffuse. The particles that are moving about randomly have their own kinetic energy.
Surface area to volume ratio
The surface area : volume ratio of an organism affects how easily substances can be exchanged between it, and its environment
Most bacteria are single-celled organisms.
Bacteria have a relatively large surface area in comparison to their volume
This means that the distance between the cell membrane at a bacterial cell's surface, and the centre of the cell, is relatively low
Substances do not have to travel very far to get where they are needed, so transport by diffusion, osmosis or active transport alone is sufficient for the cell to meet its needs
Diffusion in a bacterial cell

Calculating surface area to volume ratios
Being able to calculate and compare surface area to volume ratios is important
The effect of increasing size on surface area to volume ratio can be modelled using simple cubes
Larger organisms with smaller surface area to volume ratios need exchange surfaces and transport systems to ensure their cells obtain the materials they need
Surface area to volume ratio is calculated:

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