Investigating the Differences in Inspired & Expired Air (Cambridge (CIE) IGCSE Biology): Revision Note

Exam code: 0610 & 0970

Lára Marie McIvor

Written by: Lára Marie McIvor

Reviewed by: Jenna Quinn

Updated on

Investigating the differences in inspired & expired air

  • A simple experimental setup can be used to investigate the differences between inspired and expired air

Diagram of two limewater boiling tubes, A and B, linked by rubber tubing to the mouth to compare exhaled carbon dioxide effects on limewater.
The limewater test
  • When air is breathed in, it is drawn through boiling tube A

  • When air is breathed out, the air is blown into boiling tube B

  • Limewater is clear but becomes cloudy (or milky) when carbon dioxide is bubbled through it

    • The limewater in boiling tube A will remain clear, as inhaled air is atmospheric air, which contains a very low percentage of carbon dioxide

      The limewater in boiling tube B will become cloudy, as exhaled air has passed through the lungs (where gas exchange has taken place) and contains a higher percentage of carbon dioxide

  • This shows us that the percentage of carbon dioxide in exhaled air is higher than in inhaled air

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Lára Marie McIvor

Author: Lára Marie McIvor

Expertise: Content Creator

Lára graduated from Oxford University in Biological Sciences and has now been a science tutor working in the UK for several years. Lára has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning.

Jenna Quinn

Reviewer: Jenna Quinn

Expertise: Content Creator

Jenna studied at Cardiff University before training to become a science teacher at the University of Bath specialising in Biology (although she loves teaching all three sciences at GCSE level!). Teaching is her passion, and with 10 years experience teaching across a wide range of specifications – from GCSE and A Level Biology in the UK to IGCSE and IB Biology internationally – she knows what is required to pass those Biology exams.